cDNA Clone Sic1

• Plasmid: pYES2-GFP-StrepII.6His.TUBE.8Gly.3HA-Sic1
• Parent Plasmid: pYES2
• Expressed: GFP-StrepII.6His.TUBE.8Gly.3HA-Sic1
• Tag: GFP
• Tag: StrepII
• Tag: 6His
• Tag: 8Gly
• Tag: 3HA
• Species: Human

Sequence of Insert

GCGGCCGCgATGACTCCTTCCACCCCACCAAGGTCCAGAGGGACTAGGTA
CCTTGCGCAGCCTAGTGGCAATACTAGTTCTAGTGCCCTAATGCAAGGTC
AAAAGACCCCCCAAAAGCCTTCACAGAACCTAGTCCCTGTCACTCCCTCA
ACAACTAAGTCTTTTAAAAATGCGCCATTATTAGCACCTCCCAATTCGAA
CATGGGTATGACCTCTCCATTTAATGGGCTTACGTCTCCTCAACGCTCGC
CGTTTCCAAAATCTTCAGTGAAGAGGACACTATTCCAATTTGAAAGTCAT
GATAATGGAACAGTAAGGGAAGAGCAGGAACCATTGGGTCGTGTAAATAG
GATATTGTTTCCCACGCAGCAAAATGTGGATATAGATGCAGCAGAAGAAG
AAGAAGAAGGAGAAGTTCTTCTTCCCCCCAGCAGACCTACATCTGCCAGG
CAGTTACATTTATCACTTGAAAGAGATGAGTTTGATCAGACACATAGAAA
GAAGATTATTAAAGATGTACCTGGTACGCCCAGCGACAAGGTGATAACAT
TTGAATTGGCAAAAAATTGGAACAACAACTCTCCGAAAAATGACGCCAGG
AGTCAAGAAAGTGAAGACGAGGAAGACATCATCATCAATCCAGTGCGGGT
GGGTAAAAATCCCTTTGCATCAGATGAACTGGTCACTCAGGAAATTAGAA
ATGAACGTAAAAGGGCAATGTTGAGAGAAAACCCAGATATAGAAGACGTA
ATAACATATGTCAATAAGAAGGGAGAGGTGGTAGAGAAACGAAGGTTAAC
GGATGAAGAAAAGAGAAGATTCAAGCCAAAGGCATTGTTTCAATCTAGGG
ATCAAGAGCATTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKKLMWSHPQFEK
GAHHHHHHRSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIAT
GGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFL
NREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQ
QQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGV
NPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGS
QPSGGGGSGGGGGGGGSMYPYDVPDYAMYPYDVPDYAMYPYDVPDYAAAA
MTPSTPPRSRGTRYLAQPSGNTSSSALMQGQKTPQKPSQNLVPVTPSTTK
SFKNAPLLAPPNSNMGMTSPFNGLTSPQRSPFPKSSVKRTLFQFESHDNG
TVREEQEPLGRVNRILFPTQQNVDIDAAEEEEEGEVLLPPSRPTSARQLH
LSLERDEFDQTHRKKIIKDVPGTPSDKVITFELAKNWNNNSPKNDARSQE
SEDEEDIIINPVRVGKNPFASDELVTQEIRNERKRAMLRENPDIEDVITY
VNKKGEVVEKRRLTDEEKRRFKPKALFQSRDQEH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20823
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Vector only control equivalent is SC20621; Yeast vector for Gal1-driven expression of N-terminally tagged GFP.StrepII.6HIS.ubiquilin TUBE-8Gly-3HA-Sic1; Estimated MW of expressed protein : 91593 Daltons; Parental SC20753 (pYES2-GFP-StrepII.6HIS.TUBE.8Gly.3HA)