cDNA Clone Sic1

• Plasmid: pRS413-Gal1-StrepII.6His-Sic1
• Parent Plasmid: pRS413
• Expressed: StrepII.6His-Sic1
• Genbank: 850768
• Tag: 6His
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGACTCCTTCCACCCCACCAAGGTCCAGAGGGACTAGGTACCT
TGCGCAGCCTAGTGGCAATACTAGTTCTAGTGCCCTAATGCAAGGTCAAA
AGACCCCCCAAAAGCCTTCACAGAACCTAGTCCCTGTCACTCCCTCAACA
ACTAAGTCTTTTAAAAATGCGCCATTATTAGCACCTCCCAATTCGAACAT
GGGTATGACCTCTCCATTTAATGGGCTTACGTCTCCTCAACGCTCGCCGT
TTCCAAAATCTTCAGTGAAGAGGACACTATTCCAATTTGAAAGTCATGAT
AATGGAACAGTAAGGGAAGAGCAGGAACCATTGGGTCGTGTAAATAGGAT
ATTGTTTCCCACGCAGCAAAATGTGGATATAGATGCAGCAGAAGAAGAAG
AAGAAGGAGAAGTTCTTCTTCCCCCCAGCAGACCTACATCTGCCAGGCAG
TTACATTTATCACTTGAAAGAGATGAGTTTGATCAGACACATAGAAAGAA
GATTATTAAAGATGTACCTGGTACGCCCAGCGACAAGGTGATAACATTTG
AATTGGCAAAAAATTGGAACAACAACTCTCCGAAAAATGACGCCAGGAGT
CAAGAAAGTGAAGACGAGGAAGACATCATCATCAATCCAGTGCGGGTGGG
TAAAAATCCCTTTGCATCAGATGAACTGGTCACTCAGGAAATTAGAAATG
AACGTAAAAGGGCAATGTTGAGAGAAAACCCAGATATAGAAGACGTAATA
ACATATGTCAATAAGAAGGGAGAGGTGGTAGAGAAACGAAGGTTAACGGA
TGAAGAAAAGAGAAGATTCAAGCCAAAGGCATTGTTTCAATCTAGGGATC
AAGAGCATTGAGCGGCCGC

Amino Acid Sequence

MWSHPQFEKGAHHHHHHRSGGGGGGGGSMTPSTPPRSRGTRYLAQPSGNT
SSSALMQGQKTPQKPSQNLVPVTPSTTKSFKNAPLLAPPNSNMGMTSPFN
GLTSPQRSPFPKSSVKRTLFQFESHDNGTVREEQEPLGRVNRILFPTQQN
VDIDAAEEEEEGEVLLPPSRPTSARQLHLSLERDEFDQTHRKKIIKDVPG
TPSDKVITFELAKNWNNNSPKNDARSQESEDEEDIIINPVRVGKNPFASD
ELVTQEIRNERKRAMLRENPDIEDVITYVNKKGEVVEKRRLTDEEKRRFK
PKALFQSRDQEH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20721
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for Gal1 Promoter driven expression of Sic1 N-terminally tagged with StrepII.6HIS. ; Parental Plasmid SC20696 (pRS413 derived)