cDNA Clone Sic1

• Plasmid: pYES2-StrepII-6His.TUBE-Sic1
• Parent Plasmid: pYES2
• Expressed: StrepII.6His.TUBE-Sic1
• Genbank: 850768
• Tag: 6His
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGACTCCTTCCACCCCACCAAGGTCCAGAGGGACTAGGTACCT
TGCGCAGCCTAGTGGCAATACTAGTTCTAGTGCCCTAATGCAAGGTCAAA
AGACCCCCCAAAAGCCTTCACAGAACCTAGTCCCTGTCACTCCCTCAACA
ACTAAGTCTTTTAAAAATGCGCCATTATTAGCACCTCCCAATTCGAACAT
GGGTATGACCTCTCCATTTAATGGGCTTACGTCTCCTCAACGCTCGCCGT
TTCCAAAATCTTCAGTGAAGAGGACACTATTCCAATTTGAAAGTCATGAT
AATGGAACAGTAAGGGAAGAGCAGGAACCATTGGGTCGTGTAAATAGGAT
ATTGTTTCCCACGCAGCAAAATGTGGATATAGATGCAGCAGAAGAAGAAG
AAGAAGGAGAAGTTCTTCTTCCCCCCAGCAGACCTACATCTGCCAGGCAG
TTACATTTATCACTTGAAAGAGATGAGTTTGATCAGACACATAGAAAGAA
GATTATTAAAGATGTACCTGGTACGCCCAGCGACAAGGTGATAACATTTG
AATTGGCAAAAAATTGGAACAACAACTCTCCGAAAAATGACGCCAGGAGT
CAAGAAAGTGAAGACGAGGAAGACATCATCATCAATCCAGTGCGGGTGGG
TAAAAATCCCTTTGCATCAGATGAACTGGTCACTCAGGAAATTAGAAATG
AACGTAAAAGGGCAATGTTGAGAGAAAACCCAGATATAGAAGACGTAATA
ACATATGTCAATAAGAAGGGAGAGGTGGTAGAGAAACGAAGGTTAACGGA
TGAAGAAAAGAGAAGATTCAAGCCAAAGGCATTGTTTCAATCTAGGGATC
AAGAGCATTGAGCGGCCGC

Amino Acid Sequence

MWSHPQFEKGAHHHHHHRSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNRE
ANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQL
EQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQ
LQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS
GGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDIN
AAIERLLGSQPSGGGGSGGGGGGGGSMTPSTPPRSRGTRYLAQPSGNTSS
SALMQGQKTPQKPSQNLVPVTPSTTKSFKNAPLLAPPNSNMGMTSPFNGL
TSPQRSPFPKSSVKRTLFQFESHDNGTVREEQEPLGRVNRILFPTQQNVD
IDAAEEEEEGEVLLPPSRPTSARQLHLSLERDEFDQTHRKKIIKDVPGTP
SDKVITFELAKNWNNNSPKNDARSQESEDEEDIIINPVRVGKNPFASDEL
VTQEIRNERKRAMLRENPDIEDVITYVNKKGEVVEKRRLTDEEKRRFKPK
ALFQSRDQEH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20720
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for Gal1-driven expression of yeast Sic1 N-terminally tagged by StrepII.6HIS.ubiquilin TUBE.; Parental PLasmid SC20561; Estimated MW of expressed protein : 60569 Daltons