cDNA Clone WDR20

• Plasmid: pGEX6P-3-WDR20
• Parent Plasmid: pGEX6P3
• Expressed: GST-WDR20
• Genbank: NM_144574.2
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGGCGACGGAGGGAGGAGGGAAGGAGATGAACGAGATTAAGAC
CCAATTCACCACCCGGGAAGGTCTGTACAAGCTGCTGCCGCACTCGGAGT
ACAGCCGGCCCAACCGGGTGCCCTTCAACTCGCAGGGATCCAACCCTGTC
CGCGTCTCCTTCGTAAACCTCAACGACCAGTCTGGCAACGGCGACCGCCT
CTGCTTCAATGTGGGCCGGGAGCTGTACTTCTATATCTACAAGGGGGTCC
GCAAGGCTGCTGACTTGAGTAAACCAATAGATAAAAGGATATACAAAGGA
ACACAGCCTACTTGTCATGACTTCAACCACCTAACAGCCACAGCAGAAAG
TGTCTCTCTCCTAGTGGGCTTTTCCGCAGGCCAAGTCCAGCTTATAGACC
CAATCAAAAAAGAAACTAGCAAACTTTTTAATGAGGAAAGACTAATAGAC
AAGTCACGAGTTACCTGTGTCAAATGGGTTCCCGGTTCGGAAAGCCTTTT
CCTAGTAGCCCACTCGAGTGGGAACATGTACTTATATAATGTGGAGCACA
CTTGTGGCACCACAGCCCCCCACTACCAGCTTCTGAAGCAGGGAGAGAGC
TTTGCCGTGCACACTTGCAAGAGCAAATCCACGAGGAACCCTCTCCTTAA
GTGGACGGTGGGCGAGGGGGCCCTCAACGAGTTTGCTTTCTCCCCAGATG
GCAAGTTCTTAGCGTGCGTGAGCCAGGACGGGTTTCTGCGGGTGTTCAAC
TTTGACTCAGTGGAGCTGCACGGTACGATGAAAAGCTACTTTGGGGGCTT
GCTGTGTGTGTGCTGGAGCCCGGATGGCAAGTACATCGTGACAGGTGGGG
AGGACGACTTGGTGACAGTCTGGTCCTTTGTAGACTGCCGAGTAATAGCC
AGAGGCCACGGGCACAAGTCCTGGGTCAGTGTTGTAGCGTTTGACCCTTA
TACCACTAGTGTAGAAGAAGGTGACCCTATGGAGTTTAGTGGCAGCGATG
AGGACTTCCAAGACCTTCTTCATTTTGGCAGAGATCGAGCAAATAGTACA
CAGTCCAGGCTCTCCAAACGGAACTCTACAGACAGCCGCCCCGTAAGTGT
CACGTATCGGTTTGGTTCCGTGGGCCAGGACACACAGCTCTGTTTATGGG
ACCTTACAGAAGATATCCTTTTCCCTCACCAACCCCTCTCAAGAGCAAGG
ACACACACAAATGTCATGAATGCCACGAGTCCTCCTGCTGGAAGCAATGG
GAACAGTGTTACAACACCCGGGAACTCTGTGCCGCCTCCTCTGCCACGGT
CCAACAGCCTTCCACATTCAGCAGTCTCAAATGCTGGCAGCAAAAGCAGT
GTCATGGACGGGGCCATTGCTTCTGGGGTCAGCAAATTTGCAACACTTTC
ACTACATGACCGGAAGGAGAGGCACCACGAGAAAGATCACAAGCGAAATC
ATAGCATGGGACACATTTCTAGCAAGAGCAGTGACAAACTGAATCTAGTT
ACCAAAACCAAAACGGACCCTGCTAAAACTCTGGGAACGCCCCTGTGTCC
TCGAATGGAAGATGTTCCCTTGTTAGAGCCGCTGATATGTAAAAAGATAG
CACATGAGAGACTGACTGTACTAATATTTCTTGAAGACTGTATAGTCACT
GCTTGTCAGGAGGGATTTATTTGCACATGGGGAAGGCCTGGTAAAGTGGT
AAGTTTTAATCCTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMATEGGGKEMNEI
KTQFTTREGLYKLLPHSEYSRPNRVPFNSQGSNPVRVSFVNLNDQSGNGD
RLCFNVGRELYFYIYKGVRKAADLSKPIDKRIYKGTQPTCHDFNHLTATA
ESVSLLVGFSAGQVQLIDPIKKETSKLFNEERLIDKSRVTCVKWVPGSES
LFLVAHSSGNMYLYNVEHTCGTTAPHYQLLKQGESFAVHTCKSKSTRNPL
LKWTVGEGALNEFAFSPDGKFLACVSQDGFLRVFNFDSVELHGTMKSYFG
GLLCVCWSPDGKYIVTGGEDDLVTVWSFVDCRVIARGHGHKSWVSVVAFD
PYTTSVEEGDPMEFSGSDEDFQDLLHFGRDRANSTQSRLSKRNSTDSRPV
SVTYRFGSVGQDTQLCLWDLTEDILFPHQPLSRARTHTNVMNATSPPAGS
NGNSVTTPGNSVPPPLPRSNSLPHSAVSNAGSKSSVMDGAIASGVSKFAT
LSLHDRKERHHEKDHKRNHSMGHISSKSSDKLNLVTKTKTDPAKTLGTPL
CPRMEDVPLLEPLICKKIAHERLTVLIFLEDCIVTACQEGFICTWGRPGK
VVSFNP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20711
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST- WDR20; ; Estimated MW of expressed protein : 90387 Daltons