cDNA Clone Sic1

• Plasmid: pYES2-HA-Sic1
• Parent Plasmid: pYES2
• Expressed: HA-Sic1
• Genbank: 850768
• Tag: HA
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGACTCCTTCCACCCCACCAAGGTCCAGAGGGACTAGGTACCT
TGCGCAGCCTAGTGGCAATACTAGTTCTAGTGCCCTAATGCAAGGTCAAA
AGACCCCCCAAAAGCCTTCACAGAACCTAGTCCCTGTCACTCCCTCAACA
ACTAAGTCTTTTAAAAATGCGCCATTATTAGCACCTCCCAATTCGAACAT
GGGTATGACCTCTCCATTTAATGGGCTTACGTCTCCTCAACGCTCGCCGT
TTCCAAAATCTTCAGTGAAGAGGACACTATTCCAATTTGAAAGTCATGAT
AATGGAACAGTAAGGGAAGAGCAGGAACCATTGGGTCGTGTAAATAGGAT
ATTGTTTCCCACGCAGCAAAATGTGGATATAGATGCAGCAGAAGAAGAAG
AAGAAGGAGAAGTTCTTCTTCCCCCCAGCAGACCTACATCTGCCAGGCAG
TTACATTTATCACTTGAAAGAGATGAGTTTGATCAGACACATAGAAAGAA
GATTATTAAAGATGTACCTGGTACGCCCAGCGACAAGGTGATAACATTTG
AATTGGCAAAAAATTGGAACAACAACTCTCCGAAAAATGACGCCAGGAGT
CAAGAAAGTGAAGACGAGGAAGACATCATCATCAATCCAGTGCGGGTGGG
TAAAAATCCCTTTGCATCAGATGAACTGGTCACTCAGGAAATTAGAAATG
AACGTAAAAGGGCAATGTTGAGAGAAAACCCAGATATAGAAGACGTAATA
ACATATGTCAATAAGAAGGGAGAGGTGGTAGAGAAACGAAGGTTAACGGA
TGAAGAAAAGAGAAGATTCAAGCCAAAGGCATTGTTTCAATCTAGGGATC
AAGAGCATTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGGGGSMTPSTPPRSRGTRYLAQPSGNTSSSALMQGQ
KTPQKPSQNLVPVTPSTTKSFKNAPLLAPPNSNMGMTSPFNGLTSPQRSP
FPKSSVKRTLFQFESHDNGTVREEQEPLGRVNRILFPTQQNVDIDAAEEE
EEGEVLLPPSRPTSARQLHLSLERDEFDQTHRKKIIKDVPGTPSDKVITF
ELAKNWNNNSPKNDARSQESEDEEDIIINPVRVGKNPFASDELVTQEIRN
ERKRAMLRENPDIEDVITYVNKKGEVVEKRRLTDEEKRRFKPKALFQSRD
QEH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20656
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for Gal1-driven expression of HA-Sic1 ; Parental PLasmid SC20553; Estimated MW of expressed protein : 33754 Daltons