cDNA Clone PEX10

• Plasmid: pEFIRES-P-eYFP C1-PEX10 a159c g165t c168g
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eYFP-PEX10 a159c g165t c168g
• Genbank: NM_002617.3
• Tag: YFP
• Species: Human

Sequence of Insert

AGATCTATGGCCCCGGCCGCCGCCAGCCCCCCGGAGGTGATCCGCGCGGC
GCAGAAGGACGAGTACTACCGCGGTGGGCTGCGGAGCGCGGCGGGCGGCG
CCCTGCACAGCCTGGCGGGTGCGAGGAAGTGGCTGGAGTGGAGGAAGGAG
GTTGAGCTGCTCTCCGATGTTGCGTACTTTGGCCTCACCACACTTGCAGG
CTACCAGACCCTGGGGGAGGAGTACGTCAGCATCATCCAGGTGGACCCAT
CGCGGATACATGTGCCCTCCTCGCTGCGCCGTGGCGTGCTGGTGACACTG
CATGCCGTCCTGCCCTACCTGCTGGACAAGGCCCTGCTCCCCCTGGAGCA
GGAGCTGCAGGCTGACCCCGACAGTGGGCGACCCTTGCAGGGGAGCCTGG
GGCCAGGTGGGCGTGGCTGCTCAGGGGCGCGGCGCTGGATGCGTCACCAC
ACGGCCACCCTGACTGAGCAGCAGAGGAGGGCGCTGCTGCGGGCGGTCTT
CGTCCTCAGACAGGGCCTCGCCTGCCTCCAGCGGCTACATGTTGCCTGGT
TTTACATCCACGGTGTCTTCTACCACCTGGCCAAGAGGCTCACGGGGATC
ACGTACCTCCGTGTCCGCAGCCTGCCCGGAGAGGACCTGAGGGCCCGTGT
TAGCTACAGGCTGCTGGGGGTCATCTCACTGCTGCACCTGGTGCTGTCCA
TGGGGCTGCAGCTGTACGGTTTCAGGCAGCGGCAGCGAGCCAGGAAGGAG
TGGAGGCTGCACCGCGGCCTGTCTCACCGCAGGGCCTCCTTGGAGGAGAG
AGCCGTTTCCAGAAACCCCCTGTGCACCCTGTGCCTGGAGGAGCGCAGGC
ACCCAACAGCCACGCCCTGCGGCCACCTGTTCTGCTGGGAGTGCATCACC
GCGTGGTGCAGCAGCAAGGCGGAGTGTCCCCTCTGCCGGGAGAAGTTCCC
TCCCCAGAAGCTCATCTACCTTCGGCACTACCGCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMAPAAA
SPPEVIRAAQKDEYYRGGLRSAAGGALHSLAGARKWLEWRKEVELLSDVA
YFGLTTLAGYQTLGEEYVSIIQVDPSRIHVPSSLRRGVLVTLHAVLPYLL
DKALLPLEQELQADPDSGRPLQGSLGPGGRGCSGARRWMRHHTATLTEQQ
RRALLRAVFVLRQGLACLQRLHVAWFYIHGVFYHLAKRLTGITYLRVRSL
PGEDLRARVSYRLLGVISLLHLVLSMGLQLYGFRQRQRARKEWRLHRGLS
HRRASLEERAVSRNPLCTLCLEERRHPTATPCGHLFCWECITAWCSSKAE
CPLCREKFPPQKLIYLRHYR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20611
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for eYFP-PEX10 a159c g165t c168g. EF1a promoter, IRES and puromycin selection cassette; ; Estimated MW of expressed protein : 64544 Daltons