cDNA Clone TRAFD1

• Plasmid: pEX-GST-TRAFD1 (101-582)
• Parent Plasmid: pEX C-GST
• Expressed: TRAFD1 (101-582)-GST
• Genbank: NM_006700.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCAAGGAGATGaaactgaaggaacatgaagattattgtggtgcccggacggaactatgtggcaactgtggtcgcaatgtccttgtgaaagatctgaagactcaccctgaagtttgtgggagagagggggaggaaaagagaaatgaggttgccatacctcctaatgcatatgatgaatcttggggtcaggatggaatctggattgcatcccaactcctcagacaaattgaggctctggacccacccatgaggctgccgcgaaggcccctgagagcctttgaatcagatgttttccacaatagaactaccaaccaaaggaacattacagcccaggtttcaattcagaataatctgtttgaagaacaagagaggcaggaaaggaatagaggccaacagccccccaaagagggtggtgaagagagtgcaaacttggacttcatgttggccctaagtctgcaaaatgaaggccaagcctccagtgtggcagagcaggacttctggagggccgtatgtgaggccgaccagtctcatggcggtcccaggtctctcagtgacataaagggtgcagctgacgagatcatgttgccttgtgaattttgtgaggagctctacccagaggaactgctgattgaccatcagacaagctgtaacccttcacgtgccttaccttcactcaatactggcagctcttcccccagaggggtggaggaacctgatgtcatcttccagaacttcttgcaacaggctgcaagtaaccagtta
gactctttgatgggcctgagcaattcacaccctgtggaggagagcatcat
tatcccatgtgaattctgtggggtacagctggaagaggaggtgctgttcc
atcaccaggaccagtgtgaccaacgcccagccactgcaaccaaccatgtg
acagaggggattcctagactggattcccagcctcaagagacctcaccaga
gctgcccaggaggcgtgtcagacaccagggagacctgtcttctggttacc
tggatgatactaagcaggaaacagctaatgggcccacctcctgtctgcct
cccagccgacccattaacaatatgacagctacctataaccagctatcgag
atcaacatcaggccccagacctgggtgccagcccagctctccttgtgtgc
cgaagctcagcaactcagacagccaggacatccaggggcggaatcgagac
agccagaatggggccatagcccctgggcacgtttcagtgattcgccctcc
tcaaaatctctacccagaaaacattgtgccctctttctcccctgggcctt
cagggagatacggagctagtggtaggagtgaaggtggcaggaattcccgg
gtcacccctgcagctgccaactaccgcagcagaactgcaaaggcaaagcc
ttccaagcaacagggagctggggatgcagaagaggaagaggaggagGCGGCCGC

Amino Acid Sequence

MKLKEHEDYCGARTELCGNCGRNVLVKDLKTHPEVCGREGEEKRNEVAIP
PNAYDESWGQDGIWIASQLLRQIEALDPPMRLPRRPLRAFESDVFHNRTT
NQRNITAQVSIQNNLFEEQERQERNRGQQPPKEGGEESANLDFMLALSLQ
NEGQASSVAEQDFWRAVCEADQSHGGPRSLSDIKGAADEIMLPCEFCEEL
YPEELLIDHQTSCNPSRALPSLNTGSSSPRGVEEPDVIFQNFLQQAASNQ
LDSLMGLSNSHPVEESIIIPCEFCGVQLEEEVLFHHQDQCDQRPATATNH
VTEGIPRLDSQPQETSPELPRRRVRHQGDLSSGYLDDTKQETANGPTSCL
PPSRPINNMTATYNQLSRSTSGPRPGCQPSSPCVPKLSNSDSQDIQGRNR
DSQNGAIAPGHVSVIRPPQNLYPENIVPSFSPGPSGRYGASGRSEGGRNS
RVTPAAANYRSRTAKAKPSKQQGAGDAEEEEEEAAALEVLFQGPLSRMSP
ILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFP
NLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDF
MLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPL
QGWQATFGGGDHPPKSDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20512
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for TRAFD1 (101-582)-GST; ; Estimated MW of expressed protein : 80549 Daltons