cDNA Clone TRAFD1

• Plasmid: pGEX6P-1-TRAFD1 (101-582)
• Parent Plasmid: pGEX6P1
• Expressed: GST-TRAFD1 (101-582)
• Genbank: NM_006700.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCAAACTGAAGGAACATGAAGATTATTGTGGTGCCCGGACGGAACT
ATGTGGCAACTGTGGTCGCAATGTCCTTGTGAAAGATCTGAAGACTCACC
CTGAAGTTTGTGGGAGAGAGGGGGAGGAAAAGAGAAATGAGGTTGCCATA
CCTCCTAATGCATATGATGAATCTTGGGGTCAGGATGGAATCTGGATTGC
ATCCCAACTCCTCAGACAAATTGAGGCTCTGGACCCACCCATGAGGCTGC
CGCGAAGGCCCCTGAGAGCCTTTGAATCAGATGTTTTCCACAATAGAACT
ACCAACCAAAGGAACATTACAGCCCAGGTTTCAATTCAGAATAATCTGTT
TGAAGAACAAGAGAGGCAGGAAAGGAATAGAGGCCAACAGCCCCCCAAAG
AGGGTGGTGAAGAGAGTGCAAACTTGGACTTCATGTTGGCCCTAAGTCTG
CAAAATGAAGGCCAAGCCTCCAGTGTGGCAGAGCAGGACTTCTGGAGGGC
CGTATGTGAGGCCGACCAGTCTCATGGCGGTCCCAGGTCTCTCAGTGACA
TAAAGGGTGCAGCTGACGAGATCATGTTGCCTTGTGAATTTTGTGAGGAG
CTCTACCCAGAGGAACTGCTGATTGACCATCAGACAAGCTGTAACCCTTC
ACGTGCCTTACCTTCACTCAATACTGGCAGCTCTTCCCCCAGAGGGGTGG
AGGAACCTGATGTCATCTTCCAGAACTTCTTGCAACAGGCTGCAAGTAAC
CAGTTAGACTCTTTGATGGGCCTGAGCAATTCACACCCTGTGGAGGAGAG
CATCATTATCCCATGTGAATTCTGTGGGGTACAGCTGGAAGAGGAGGTGC
TGTTCCATCACCAGGACCAGTGTGACCAACGCCCAGCCACTGCAACCAAC
CATGTGACAGAGGGGATTCCTAGACTGGATTCCCAGCCTCAAGAGACCTC
ACCAGAGCTGCCCAGGAGGCGTGTCAGACACCAGGGAGACCTGTCTTCTG
GTTACCTGGATGATACTAAGCAGGAAACAGCTAATGGGCCCACCTCCTGT
CTGCCTCCCAGCCGACCCATTAACAATATGACAGCTACCTATAACCAGCT
ATCGAGATCAACATCAGGCCCCAGACCTGGGTGCCAGCCCAGCTCTCCTT
GTGTGCCGAAGCTCAGCAACTCAGACAGCCAGGACATCCAGGGGCGGAAT
CGAGACAGCCAGAATGGGGCCATAGCCCCTGGGCACGTTTCAGTGATTCG
CCCTCCTCAAAATCTCTACCCAGAAAACATTGTGCCCTCTTTCTCCCCTG
GGCCTTCAGGGAGATACGGAGCTAGTGGTAGGAGTGAAGGTGGCAGGAAT
TCCCGGGTCACCCCTGCAGCTGCCAACTACCGCAGCAGAACTGCAAAGGC
AAAGCCTTCCAAGCAACAGGGAGCTGGGGATGCAGAAGAGGAAGAGGAGG
AGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSKLKEHEDYCGARTELCGNC
GRNVLVKDLKTHPEVCGREGEEKRNEVAIPPNAYDESWGQDGIWIASQLL
RQIEALDPPMRLPRRPLRAFESDVFHNRTTNQRNITAQVSIQNNLFEEQE
RQERNRGQQPPKEGGEESANLDFMLALSLQNEGQASSVAEQDFWRAVCEA
DQSHGGPRSLSDIKGAADEIMLPCEFCEELYPEELLIDHQTSCNPSRALP
SLNTGSSSPRGVEEPDVIFQNFLQQAASNQLDSLMGLSNSHPVEESIIIP
CEFCGVQLEEEVLFHHQDQCDQRPATATNHVTEGIPRLDSQPQETSPELP
RRRVRHQGDLSSGYLDDTKQETANGPTSCLPPSRPINNMTATYNQLSRST
SGPRPGCQPSSPCVPKLSNSDSQDIQGRNRDSQNGAIAPGHVSVIRPPQN
LYPENIVPSFSPGPSGRYGASGRSEGGRNSRVTPAAANYRSRTAKAKPSK
QQGAGDAEEEEEE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20511
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct for expression of GST-TRAFD1 (residues 101-582) ; ; Estimated MW of expressed protein : 79992 Daltons