cDNA Clone Ubiquitin

• Plasmid: pcDNA3.1 DAC-A-Ubiquitin G75A G76A
• Parent Plasmid: pcDNA3.1 DAC 3C
• Expressed: DAC-A-Ubiquitin G75A G76A
• Genbank: P62988
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCGGCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGG
CCGC

Amino Acid Sequence

MIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQA
MKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGIN
LQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADG
QYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSL
NVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLT
WGFRFFETVNPLEVLFQGPGSGAMQIFVKTLTGKTITLEVEPSDTIENVK
AKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRAA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20418
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bam-Not fragment harbouring C-terminal single ubiquitin unit with C-terminal AA instead of GG and STOP codon. ; Plasmid for mammalian expression of N-terminally DAC-A tagged monoubiquitin (contains precission site); ; Estimated MW of expressed protein : 38209 Daltons; Parental Plasmid DU30209