cDNA Clone VCP

• Synonyms: p97
• Plasmid: pCMV5 VCP-FLAG P137L
• Parent Plasmid: pCMV5
• Expressed: VCP-FLAG P137L
• Genbank: NM_007126
• Tag: FLAG
• Species: Human

Sequence of Insert

AAGCTTGCCACCATGGCTTCTGGAGCCGATTCAAAAGGTGATGACCTATC
AACAGCCATTCTCAAACAGAAGAACCGTCCCAATCGGTTAATTGTTGATG
AAGCCATCAATGAGGACAACAGTGTGGTGTCCTTGTCCCAGCCCAAGATG
GATGAATTGCAGTTGTTCCGAGGTGACACAGTGTTGCTGAAAGGAAAGAA
GAGACGAGAAGCTGTTTGCATCGTCCTTTCTGATGATACTTGTTCTGATG
AGAAGATTCGGATGAATAGAGTTGTTCGGAATAACCTTCGTGTACGCCTA
GGGGATGTCATCAGCATCCAGCCATGCCCTGATGTGAAGTACGGCAAACG
TATCCATGTGCTGCCCATTGATGACACAGTGGAAGGCATTACTGGTAATC
TCTTCGAGGTATACCTTAAGCTGTACTTCCTGGAAGCGTATCGACCCATC
CGGAAAGGAGACATTTTTCTTGTCCGTGGTGGGATGCGTGCTGTGGAGTT
CAAAGTGGTGGAAACAGATCCTAGCCCTTATTGCATTGTTGCTCCAGACA
CAGTGATCCACTGCGAAGGGGAGCCTATCAAACGAGAGGATGAGGAAGAG
TCCTTGAATGAAGTAGGGTATGATGACATTGGTGGCTGCAGGAAGCAGCT
AGCTCAGATAAAGGAGATGGTGGAACTGCCCCTGAGACATCCTGCCCTCT
TTAAGGCAATTGGTGTGAAGCCTCCTAGAGGAATCCTGCTTTACGGACCT
CCTGGAACAGGAAAGACCCTGATTGCTCGAGCTGTAGCAAATGAGACTGG
AGCCTTCTTCTTCTTGATCAATGGTCCTGAGATCATGAGCAAATTGGCTG
GTGAGTCTGAGAGCAACCTTCGTAAAGCCTTTGAGGAGGCTGAGAAGAAT
GCTCCTGCCATCATCTTCATTGATGAGCTAGATGCCATCGCTCCCAAAAG
AGAGAAAACTCATGGCGAGGTGGAGCGGCGCATTGTATCACAGTTGTTGA
CCCTCATGGATGGCCTAAAGCAGAGGGCACATGTGATTGTTATGGCAGCA
ACCAACAGACCCAACAGCATTGACCCAGCTCTACGGCGATTTGGTCGCTT
TGACAGGGAGGTAGATATTGGAATTCCTGATGCTACAGGACGCTTAGAGA
TTCTTCAGATCCATACCAAGAACATGAAGCTGGCAGATGATGTGGACCTG
GAACAGGTAGCCAATGAGACTCACGGGCATGTGGGTGCTGACTTAGCAGC
CCTGTGCTCAGAGGCTGCTCTGCAAGCCATCCGCAAGAAGATGGATCTCA
TTGACCTAGAGGATGAGACCATTGATGCCGAGGTCATGAACTCTCTAGCA
GTTACTATGGATGACTTCCGGTGGGCCTTGAGCCAGAGTAACCCATCAGC
ACTGCGGGAAACCGTGGTAGAGGTGCCACAGGTAACCTGGGAAGACATCG
GGGGCCTAGAGGATGTCAAACGTGAGCTACAGGAGCTGGTCCAGTATCCT
GTGGAGCACCCAGACAAATTCCTGAAGTTTGGCATGACACCTTCCAAGGG
AGTTCTGTTCTATGGACCTCCTGGCTGTGGGAAAACTTTGTTGGCCAAAG
CCATTGCTAATGAATGCCAGGCCAACTTCATCTCCATCAAGGGTCCTGAG
CTGCTCACCATGTGGTTTGGGGAGTCTGAGGCCAATGTCAGAGAAATCTT
TGACAAGGCCCGCCAAGCTGCCCCCTGTGTGCTATTCTTTGATGAGCTGG
ATTCGATTGCCAAGGCTCGTGGAGGTAACATTGGAGATGGTGGTGGGGCT
GCTGACCGAGTCATCAACCAGATCCTGACAGAAATGGATGGCATGTCCAC
AAAAAAAAATGTGTTCATCATTGGCGCTACCAACCGGCCTGACATCATTG
ATCCTGCCATCCTCAGACCTGGCCGTCTTGATCAGCTCATCTACATCCCA
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA
GTCCCCAGTTGCCAAGGATGTGGACTTGGAGTTCCTGGCTAAAATGACTA
ATGGCTTCTCTGGAGCTGACCTGACAGAGATTTGCCAGCGTGCTTGCAAG
CTGGCCATCCGTGAATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAG
GCAGACAAACCCATCAGCCATGGAGGTAGAAGAGGATGATCCAGTGCCTG
AGATCCGTCGAGATCACTTTGAAGAAGCCATGCGCTTTGCGCGCCGTTCT
GTCAGTGACAATGACATTCGGAAGTATGAGATGTTTGCCCAGACCCTTCA
GCAGAGTCGGGGCTTTGGCAGCTTCAGATTCCCTTCAGGGAACCAGGGTG
GAGCTGGCCCCAGTCAGGGCAGTGGAGGCGGCACAGGTGGCAGTGTATAC
ACAGAAGACAATGATGATGACCTGTATGGCGACTACAAGGACGACGATGA
CAAGTAAGGATCC

Amino Acid Sequence

MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQ
LFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVI
SIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKLYFLEAYRPIRKGD
IFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNE
VGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTG
KTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAI
IFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRP
NSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVA
NETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMD
DFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHP
DKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTM
WFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRV
INQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDE
KSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIR
ESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDN
DIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDN
DDDLYGDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20069
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Plasmid for mammalian expression of C-terminally FLAG tagged VCP (P137L mutant); ; Estimated MW of expressed protein : 90334 Daltons