cDNA Clone OTUB1

• Synonyms: OTB1, OTU1
• Plasmid: pBabe.puro-HA OTUB1 C91S sprot
• Parent Plasmid: pBabeD puro HA1
• Expressed: HA-OTUB1 C91S siKO.1 + siKO.3
• Genbank: NM_017670.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGGAGGAACCTCAGCAGCAGAAGCAGGAGCCGCTGGGCAGCGACTCCGAAGGTGTTAACTGTCTGGCCTATGATGAAGCCATCATGGCTCAGCAGGACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCTGGTGTCAGAGCGGCTGGAGCTCTCGGTCCTATACAAGGAGTATGCTGAAGATGACAACATCTATCAACAGAAGATCAAGGACCTCCACAAAAAGTACTCGTACATCCGCAAGACCAGGCCTGACGGCAACAGTTTCTATCGGGCTTTCGGATTCTCCCACTTGGAGGCACTGCTGGATGACAGCAAGGAGTTGCAGCGGTTCAAGGCTGTGTCTGCCAAGAGCAAGGAAGACCTGGTGTCCCAGGGCTTCACTGAATTCACAATTGAGGATTTCCACAACACGTTCATGGACCTGATTGAGCAGGTGGAGAAGCAGACCTCTGTCGCCGACCTGCTGGCCTCCTTCAATGACCAGAGCACCTCCGATTATTTAGTAGTCTACCTGCGGCTGCTCACCTCGGGCTACCTGCAGCGCGAGAGCAAATTCTTTGAACATTTCATCGAGGGTGGACGGACTGTCAAGGAGTTCTGCCAGCAGGAGGTGGAGCCCATGTGCAAGGAGAGCGACCACATCCACATCATTGCGCTGGCCCAGGCCCTCAGCGTGTCCATCCAGGTGGAGTACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCACATCTTCCCTGAGGGCTCCGAGCCCAAGGTCTACCTTCTCTACCGGCCTGGACACTACGATATCCTCTACAAATAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNSFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU41004
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains 9 silent errors to negate binding by siRNA.1 and siRNA.3 and contains a C91/S mutation