cDNA Clone PKM zeta (rat)

• Plasmid: pFBHTb PKM zeta rat
• Parent Plasmid: pFastBac HTb
• Expressed: 6HIS PKM zeta rat
• Genbank: NP_071952.1
• Tag: 6His
• Species: Rat

Sequence of Insert

GGATCCATGGACTCCGTGATGCCCTCCCAAGAGCCCCCCGTGGACGACAAGAACGACGGTGTCGACCTGCCCTCCGAGGAAACCGACGGTATCGCTTACATCTCCTCCAGCCGCAAGCACGACAACATCAAGGACGACTCCGAGGACCTGAAGCCCGTGATCGACGGCGTGGACGGTATCAAGATCTCCCAGGGACTGGGCCTGCAGGACTTCGACCTGATCCGTGTGATCGGTCGTGGTTCCTACGCTAAGGTGCTGCTCGTGCGTCTGAAGAAGAACGACCAGATCTACGCTATGAAGGTGGTCAAGAAGGAACTGGTCCACGACGACGAGGACATCGACTGGGTGCAGACCGAGAAGCACGTGTTCGAGCAGGCTTCCAGCAACCCCTTCCTCGTGGGACTGCACTCCTGCTTCCAGACTACCTCCCGTCTGTTCCTGGTCATCGAGTACGTGAACGGCGGCGACCTGATGTTCCACATGCAGCGTCAGCGCAAGCTGCCCGAGGAACACGCTCGTTTCTACGCTGCTGAGATCTGTATCGCTCTGAACTTCCTGCACGAGCGCGGTATCATCTACCGTGACCTCAAGCTGGACAACGTGCTGCTGGACGCTGACGGTCACATCAAGCTGACCGACTACGGCATGTGCAAGGAAGGCCTGGGTCCCGGCGACACCACCTCCACTTTCTGCGGCACCCCCAACTACATCGCTCCCGAGATCCTGCGTGGCGAGGAATACGGTTTCTCCGTGGATTGGTGGGCTCTGGGCGTGCTGATGTTCGAGATGATGGCTGGTCGCTCCCCCTTCGACATCATCACCGACAACCCCGACATGAACACCGAGGACTACCTGTTCCAAGTGATCCTCGAGAAGCCTATCCGTATCCCCCGTTTCCTGTCCGTGAAGGCTTCCCACGTGCTGAAGGGATTCCTCAACAAGGACCCCAAGGAACGTCTGGGTTGCCGTCCCCAGACCGGCTTCTCCGACATCAAGTCCCACGCTTTCTTCCGTTCCATCGATTGGGACCTGCTCGAGAAGAAGCAGACCCTGCCCCCATTCCAGCCCCAGATCACCGACGACTACGGACTGGACAACTTCGACACCCAGTTCACCTCCGAGCCCGTGCAGCTGACTCCCGACGATGAGGACGTGATCAAGCGTATCGACCAGTCCGAGTTCGAGGGTTTCGAGTACATCAACCCCCTGCTGCTGTCCGCTGAAGAGTCCGTCTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMDSVMPSQEPPVDDKNDGVDLPSEETDGIAYISSSRKHDNIKDDSEDLKPVIDGVDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGMCKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTGFSDIKSHAFFRSIDWDLLEKKQTLPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSAEESV*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU43253
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: starts at amino acid 184 of referenced NCBI