cDNA Clone TRPM6

• Plasmid: pcDNAfrtTOFLAG-TRPM6 800-end
• Parent Plasmid: pcDNA5 FRT/TO FLAG1
• Expressed: Flag
• Genbank: NM_017662
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccTATGATTTGGAAAGGGGCCATGATGAGAAACTGGATGAAAATCAGCATTTTGGTTTGGAAAGTGGGCACCAACACCTTCCGTGGACCAGGAAAGTCTATGAGTTCTACAGTGCTCCAATTGTCAAGTTTTGGTTTTATACGATGGCGTATTTGGCATTCCTCATGCTGTTCACTTACACCGTGTTGGTGGAGATGCAGCCCCAGCCCAGCGTGCAGGAGTGGCTTGTTAGCATTTACATCTTCACCAATGCTATTGAGGTGGTCAGGGAGATCTGTATTTCAGAACCTGGGAAGTTTACCCAAAAGGTGAAGGTATGGATTAGTGAGTACTGGAACTTAACAGAAACTGTGGCCATTGGCCTGTTTTCAGCTGGCTTCGTCCTTCGATGGGGTGACCCTCCTTTTCACACAGCGGGAAGACTGATCTACTGCATAGACATCATATTCTGGTTCTCACGGCTCCTGGACTTCTTTGCTGTGAATCAACATGCAGGTCCATATGTGACCATGATTGCAAAAATGACAGCAAACATGTTCTATATTGTGATCATCATGGCCATAGTCCTGCTGAGCTTTGGAGTGGCACGCAAGGCCATCCTTTCGCCAAAAGAGCCACCATCTTGGAGTCTAGCTCGAGATATTGTATTTGAGCCATACTGGATGATATACGGAGAAGTCTATGCTGGAGAAATAGATGTTTGTTCAAGCCAGCCATCCTGCCCTCCTGGTTCTTTTCTTACTCCATTCTTGCAAGCTGTCTACCTCTTCGTGCAATATATCATCATGGTGAACCTGTTGATTGCTTTCTTCAACAACGTTTACTTAGATATGGAATCCATTTCAAATAACCTGTGGAAATACAACCGCTATCGCTACATCATGACCTACCACGAGAAGCCCTGGCTGCCCCCACCTCTCATCCTGCTGAGCCACGTGGGCCTTCTCCTCCGCCGCCTGTGCTGTCATCGAGCTCCTCACGACCAAGAAGAGGGTGACGTTGGATTAAAACTCTACCTCAGTAAGGAGGATCTGAAAAAACTTCATGATTTTGAGGAGCAGTGCGTGGAAAAATACTTCCATGAGAAGATGGAAGATGTGAATTGTAGTTGTGAGGAACGAATCCGAGTGACATCAGAAAGGGTTACAGAGATGTACTTCCAGCTGAAAGAAATGAATGAAAAGGTGTCTTTTATAAAGGACTCCTTACTGTCTTTGGACAGCCAGGTGGGACACCTGCAGGATCTCTCTGCCCTGACTGTGGATACCCTGAAAGTCCTTTCTGCTGTTGACACTTTGCAAGAGGATGAGGCTCTCCTGGCCAAGAGAAAGCATTCTACTTGCAAAAAACTTCCCCACAGCTGGAGCAATGTCATCTGTGCAGAGGTTCTAGGCAGCATGGAGATCGCTGGAGAGAAGAAATACCAGTATTATAGCATGCCCTCTTCTTTGCTGAGGAGCCTGGCTGGAGGCCGGCATCCCCCAAGAGTGCAGAGGGGGGCACTTCTTGAGATTACAAACAGTAAAAGAGAGGCTACAAATGTAAGAAATGACCAGGAAAGGCAAGAAACACAAAGTAGTATAGTGGTTTCTGGGGTGTCTCCTAACAGGCAAGCACACTCAAAGTATGGCCAGTTTCTTCTGGTCCCCTCTAATCTAAAGCGAGTTCCTTTTTCAGCAGAAACTGTCTTGCCTCTGTCCAGACCCTCTGTGCCAGATGTGCTGGCAACTGAACAGGACATCCAGACTGAGGTTCTTGTTCATCTGACTGGGCAGACCCCAGTTGTCTCTGACTGGGCATCAGTGGATGAACCCAAGGAAAAGCACGAGCCTATTGCTCACTTACTGGATGGACAAGACAAGGCAGAGCAAGTGCTACCCACTTTGAGTTGCACACCTGAACCCATGACAATGAGCTCCCCTCTTTCCCAAGCCAAGATCATGCAAACTGGAGGTGGATATGTAAACTGGGCATTTTCAGAAGGTGATGAAACTGGTGTGTTTAGCATCAAGAAAAAGTGGCAAACCTGCTTGCCCTCCACTTGTGACAGTGATTCCTCTCGGAGTGAACAGCACCAGAAGCAGGCCCAGGACAGCTCCCTATCTGATAACTCAACAAGATCGGCCCAGAGTAGTGAATGCTCAGAGGTGGGACCATGGCTTCAGCCAAACACATCCTTTTGGATCAATCCTCTCCGCAGATACAGGCCCTTCGCTAGGAGTCATAGTTTTAGATTCCATAAGGAGGAGAAATTGATGAAGATCTGTAAGATTAAAAATCTTTCAGGCTCTTCAGAAATAGGGCAGGGAGCATGGGTCAAAGCGAAAATGCTAACCAAAGACAGGAGACTGTCAAAGAAAAAGAAGAATACTCAAGGACTCCAGGTGCCAATCATAACAGTCAATGCCTGCTCTCAGAGTGACCAGTTGAATCCAGAGCCAGGAGAAAACAGCATCTCTGAAGAGGAGTACAGCAAGAACTGGTTCACAGTGTCCAAATTTAGTCACACAGGTGTAGAACCTTACATACATCAGAAAATGAAAACTAAAGAAATTGGACAATGTGCTATACAAATCAGTGATTACCTAAAGCAGTCTCAAGAGGATCTCAGCAAAAACTCTTTGTGGAATTCCAGGAGCACCAACCTCAATAGGAACTCCCTGCTGAAAAGTTCAATTGGAGTTGACAAGATCTCAGCCTCCTTAAAAAGCCCTCAAGAGCCTCACCATCATTATTCAGCCATTGAAAGGAATAATTTAATGAGGCTTTCTCAGACCATACCATTTACACCAGTCCAACTGTTTGCAGGAGAAGAAATAACTGTCTACAGGTTGGAGGAGAGTTCCCCTTTAAACCTTGATAAAAGCATGTCCTCTTGGTCTCAGCGTGGGAGAGCGGCAATGATCCAGGTATTGTCCCGAGAGGAGATGGATGGGGGCCTCCGTAAAGCTATGAGAGTCGTCAGCACTTGGTCTGAGGATGACATTCTCAAGCCGGGACAAGTTTTCATTGTCAAGTCCTTTCTTCCTGAGGTTGTGCGGACATGGCATAAAATCTTCCAGGAGAGCACTGTGCTTCATCTTTGCCTCAGGGAAATTCAACAACAAAGAGCTGCTCAAAAATTGATCTATACCTTCAACCAAGTGAAACCACAAACCATACCCTACACACCAAGGTTCCTGGAAGTTTTCTTAATCTACTGCCATTCAGCCAACCAGTGGTTGACCATTGAGAAGTATATGACAGGGGAGTTCCGGAAGTATAACAACAACAATGGTGATGAAATCACCCCCACCAACACCCTGGAGGAGCTGATGTTGGCTTTCTCTCACTGGACCTATGAGTACACTCGGGGAGAGCTGCTGGTTTTAGATTTGCAAGGTGTTGGAGAAAATTTGACAGATCCATCTGTTATAAAACCTGAAGTCAAACAATCAAGAGGAATGGTGTTTGGACCGGCCAATTTGGGGGAAGATGCAATTAGAAACTTCATTGCAAAACATCATTGTAACTCCTGCTGCCGGAAGCTCAAACTCCCGGATTTAAAAAGAAATGACTATTCCCCTGAAAGGATAAATTCCACCTTTGGACTTGAGATAAAAATAGAATCAGCTGAGGAGCCTCCAGCAAGGGAGACGGGTAGAAATTCCCCAGAAGATGATATGCAACTATAAgcggccgc

Amino Acid Sequence

GSYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42312
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 800-2022(end), in pcDNA5-FRT/TO; ; ; All TRPM6 plasmids MUST be grown at 30 degrees C or less to prevent recombination.