cDNA Clone UbcH7

• Synonyms: E2-F1, L-UBC, UBE2L3, UbcM4
• Plasmid: pET156P UBCH7 C86S P88D H119D
• Parent Plasmid: pET15b 6His 3C
• Expressed: His UBCH7 C86S P88D H119D
• Genbank: AAH53368
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAAAACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAGGGGCAGGTCTcTCTGgatGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGgACCCGCTTCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAAGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAA

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVSLDVISAENWKPATKTDQVIQSLIALVNDPQPEDPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD*AA

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU22363
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation a423g