cDNA Clone SYTI

• Plasmid: pCDNA3-pHluorin-SYTI K327A
• Parent Plasmid: pcDNA3-pHluorin
• Expressed: pHluorin SYTI K327A
• Genbank: NM_005639.2
• Tag: pHluorin
• Species: Human

Sequence of Insert

atggtgagcgagagtcaccatgaggccctggcagccccgcctgtcaccac
tgtcgcgactgttctgccaagcaacgccacagagccagccagtcctggag
aaggaaaggaagatgcattttctaagctgaaggagaagtttatgaatgag
ttgcataaaattccattgccaccgtgggccttaattgcaatagccatagt
cgcagtccttttagtcctgacctgctgcttttgtatctgtaagaaatgtt
tgttcaaaaagaaaaacaagaagaagggaaaggaaaaaggagggaagaat
gccattaacatgaaagatgtaaaagacttagggaagacgatgaaagatca
ggccctcaaggatgatgatgctgaaactggattgacagatggagaagaaa
aagaagaacccaaagaagaggagaaactgggaaaacttcagtattcactg
gattatgatttccaaaataaccagctgctggtagggatcattcaggctgc
cgaactgcccgccttggacatggggggcacatctgatccttacgtgaaag
tgtttctgctacctgataagaagaagaaatttgagacaaaagtccaccga
aaaacccttaatcctgtcttcaatgagcaatttactttcaaggtaccata
ctcggaattgggtggcaaaaccctagtgatggctgtatatgattttgatc
gtttctctaagcatgacatcattggagaatttaaagtccctatgaacaca
gtggattttggccatgtaactgaggaatggcgtgacctgcaaagtgctga
gaaggaagagcaagagaaattgggtgatatctgcttctcccttcgctacg
tacctactgctggtaagctgactgttgtcattctggaggcaaagaacctg
aagaagatggatgtgggtggcttatccgatccttatgtgaagattcatct
gatgcagaatggtaagaggctgaagaagGCaaagacaacaattaaaaaga
acacacttaacccctactacaatgagtcattcagctttgaagtacctttt
gaacaaatccagaaagtgcaggtggtggtaactgttttggactatgacaa
gattggcaagaacgatgccatcggcaaagtctttgtgggctacaacagca
ccggcgcggagctgcgacactggtcagacatgctggccaaccccaggcga
cctattgcccagtggcacaccctgcaggtagaggaggaagttgatgccat
gctggccgtcaagaagtaa

Amino Acid Sequence

MKILVAVAVFFLVSTQLFAEEIGANKLSKGEELFTGVVPILVELDGDVNG
HKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRY
PDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
LKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANFKIRHNIEDG
GVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFV
TAAGITHGMDELYKTGDYDIPTTLENLYFQGELKTVDADMVSESHHEALA
APPVTTVATVLPSNATEPASPGEGKEDAFSKLKEKFMNELHKIPLPPWAL
IAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINMKDVKDLG
KTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLV
GIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQF
TFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWR
DLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDP
YVKIHLMQNGKRLKKAKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVT
VLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVE
EEVDAMLAVKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42508
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee