cDNA Clone VCPIP1

• Synonyms: DUBA3, VCIP135
• Plasmid: pGEX6P-1 VCPIP1 25-561
• Parent Plasmid: pGEX6P1
• Expressed: GST-VCPIP1 25-561
• Genbank: NM_025054.4
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGTCGTCCTTGGCGTCGGCGGCTGCTTCGGGGGGGCTTTTGAAGCGGAGAGACCGGAGAATCCTTTCCGGGAGCTGCCCGGATCCGAAGTGTCAGGCGCGTCTATTTTTCCCGGCCTCCGGTTCTGTCAGCATCGAGTGTACCGAGTGCGGCCAGCGGCACGAGCAGCAACAGCTGCTGGGGGTTGAGGAGGTGACCGACCCGGACGTAGTGCTACACAACCTGCTGCGGAACGCGCTGCTCGGGGTTACGGGGGCACCCAAGAAGAACACGGAACTGGTAAAGGTGATGGGCCTTTCCAACTATCACTGCAAATTGTTGTCGCCCATATTAGCTCGCTATGGAATGGACAAACAGACAGGCCGGGCCAAGCTTCTCCGGGACATGAACCAGGGCGAACTGTTCGATTGCGCCTTACTGGGTGACCGCGCCTTCCTCATAGAACCAGAGCATGTTAACACTGTGGGCTATGGCAAGGACCGCTCCGGAAGCCTCCTGTATTTGCATGACACTCTGGAGGACATTAAGCGGGCCAATAAAAGCCAGGAATGTCTCATTCCAGTGCATGTGGACGGGGATGGACACTGCTTGGTGCATGCTGTGTCTCGGGCTCTAGTAGGCCGAGAGCTCTTCTGGCATGCCTTAAGAGAGAATCTTAAACAGCACTTTCAGCAGCACCTGGCCCGATATCAAGCTCTGTTCCATGACTTCATTGATGCTGCTGAGTGGGAGGACATTATCAATGAGTGTGACCCTCTGTTTGTACCACCTGAGGGTGTTCCCTTGGGCCTGAGGAATATCCACATATTTGGTCTTGCCAATGTGCTACATCGTCCTATTATTCTGTTAGATTCCCTCAGTGGCATGAGAAGCTCTGGTGATTATTCAGCCACCTTTCTACCTGGGCTCATCCCTGCAGAGAAGTGCACTGGGAAAGATGGTCATTTGAACAAACCAATCTGTATTGCATGGAGCAGCTCCGGTAGAAACCATTATATCCCCTTGGTAGGCATAAAAGGGGCTGCTTTGCCCAAACTGCCTATGAATTTGCTTCCTAAAGCATGGGGTGTGCCTCAGGACCTTATTAAAAAGTACATAAAACTTGAAGAGGATGGTGGTTGTGTTATTGGAGGTGACAGAAGTTTGCAAGATAAATACTTACTTAGGCTTGTTGCTGCTATGGAAGAAGTCTTTATGGACAAACATGGTATCCATCCTAGTTTGGTTGCTGATGTCCATCAGTATTTCTACAGAAGGACTGGAGTGATAGGAGTTCAGCCTGAGGAAGTCACAGCAGCTGCTAAAAAAGCAGTAATGGATAATCGCCTTCACAAATGTTTGCTCTGTGGTGCCCTTTCTGAACTTCATGTTCCTCCAGAGTGGTTGGCTCCTGGAGGGAAATTGTATAACCTGGCAAAAAGTACTCATGGACAGCTGAGGACTGACAAAAATTACAGCTTTCCCTTGAACAATTTGGTTTGCTCATATGATTCAGTGAAAGATGTTCTGGTACCAGACTATGGAATGAGTAACCTAACAGCTTGTAATTGGTGCCATGGCACATCTGTGCGAAAGGTCAGAGGAGATGGGTCTATTGTGTATTTGGATGGAGACTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMSSLASAAASGGLLKR
RDRRILSGSCPDPKCQARLFFPASGSVSIECTECGQRHEQQQLLGVEEVT
DPDVVLHNLLRNALLGVTGAPKKNTELVKVMGLSNYHCKLLSPILARYGM
DKQTGRAKLLRDMNQGELFDCALLGDRAFLIEPEHVNTVGYGKDRSGSLL
YLHDTLEDIKRANKSQECLIPVHVDGDGHCLVHAVSRALVGRELFWHALR
ENLKQHFQQHLARYQALFHDFIDAAEWEDIINECDPLFVPPEGVPLGLRN
IHIFGLANVLHRPIILLDSLSGMRSSGDYSATFLPGLIPAEKCTGKDGHL
NKPICIAWSSSGRNHYIPLVGIKGAALPKLPMNLLPKAWGVPQDLIKKYI
KLEEDGGCVIGGDRSLQDKYLLRLVAAMEEVFMDKHGIHPSLVADVHQYF
YRRTGVIGVQPEEVTAAAKKAVMDNRLHKCLLCGALSELHVPPEWLAPGG
KLYNLAKSTHGQLRTDKNYSFPLNNLVCSYDSVKDVLVPDYGMSNLTACN
WCHGTSVRKVRGDGSIVYLDGD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44386
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: residues 25-561, precission cleavage site between GST and protein