cDNA Clone Traf6

• Plasmid: pCMVHA-1 Traf6 N109A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA Traf6 N109A
• Genbank: Q9Y4K3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCagtctgctaaactgtgaaaacagctgtggattcagccagtctga
aagtgactgctgtgtggccatggccagctcctgtagcgctgtaacaaaag
atgatagtgtgggtggaactgccagcacggggaacctctccagctcattt
atggaggagatccagggatatgatgtagagtttgacccacccctggaaag
caagtatgaatgccccatctgcttgatggcattacgagaagcagtgcaaa
cgccatgcggccataggttctgcaaagcctgcatcataaaatcaataagg
gatgcaggtcacaaatgtccagttgacGCCgaaatactgctggaaaatca
actatttccagacaattttgcaaaacgtgagattctttctctgatggtga
aatgtccaaatgaaggttgtttgcacaagatggaactgagacatcttgag
gatcatcaggcacattgtgagtttgctcttatggattgtccccaatgcca
gcgtcccttccaaaaattccatattaatattcacattctgaaggattgtc
caaggagacaggtttcttgtgacaactgtgctgcatcaatggcatttgaa
gataaagagatccatgaccagaactgtcctttggcaaatgtcatctgtga
atactgcaatactatactcatcagagaacagatgcctaatcattatgatc
tagactgccctacagccccaattccatgcacattcagtacttttggttgc
catgaaaagatgcagaggaatcacttggcacgccacctacaagagaacac
ccagtcacacatgagaatgttggcccaggctgttcatagtttgagcgtta
tacccgactctgggtatatctcagaggtccggaatttccaggaaactatt
caccagttagagggtcgccttgtaagacaagaccatcaaatccgggagct
gactgctaaaatggaaactcagagtatgtatgtaagtgagctcaaacgaa
ccattcgaacccttgaggacaaagttgctgaaatcgaagcacagcagtgc
aatggaatttatatttggaagattggcaactttggaatgcatttgaaatg
tcaagaagaggagaaacctgttgtgattcatagccctggattctacactg
gcaaacccgggtacaaactgtgcatgcgcttgcaccttcagttaccgact
gctcagcgctgtgcaaactatatatccctttttgtccacacaatgcaagg
agaatatgacagccacctcccttggcccttccagggtacaatacgcctta
caattcttgatcagtctgaagcacctgtaaggcaaaaccacgaagagata
atggatgccaaaccagagctgcttgctttccagcgacccacaatcccacg
gaacccaaaaggttttggctatgtaacttttatgcatctggaagccctaa
gacaaagaactttcattaaggatgacacattattagtgcgctgtgaggtc
tccacccgctttgacatgggtagccttcggagggagggttttcagccacg
aagtactgatgcaggggtataggcggccgc

Amino Acid Sequence

MYPYDVPDYAGSSLLNCENSCGFSQSESDCCVAMASSCSAVTKDDSVGGT
ASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRF
CKACIIKSIRDAGHKCPVDAEILLENQLFPDNFAKREILSLMVKCPNEGC
LHKMELRHLEDHQAHCEFALMDCPQCQRPFQKFHINIHILKDCPRRQVSC
DNCAASMAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAP
IPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYI
SEVRNFQETIHQLEGRLVRQDHQIRELTAKMETQSMYVSELKRTIRTLED
KVAEIEAQQCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKL
CMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSE
APVRQNHEEIMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK
DDTLLVRCEVSTRFDMGSLRREGFQPRSTDAGV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU43636
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: mutation of N108 to A