cDNA Clone WNK1

• Synonyms: HSAN2, HSN2, KDP, PRKWNK1, PSK, p65
• Plasmid: pGEX6P-1 WNK1 1-661 D368A
• Parent Plasmid: pGEX6P1
• Expressed: GST-WNK1 1-661 D368A
• Genbank: NM_018979.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCTGGCGGCGCCGCAGAGAAGCAGAGCAGCACTCCCGGTTC
CCTGTTCCTCTCGCCGCCGGCTCCTGCCCCCAAGAATGGCTCCAGCTCCG
ATTCCTCCGTGGGGGAGAAACTGGGAGCCGCGGCCGCCGACGCTGTGACC
GGCAGGACCGAGGAGTACAGGCGCCGCCGCCACACTATGGACAAGGACAG
CCGTGGGGCGGCCGCGACCACTACCACCACTGAGCACCGCTTCTTCCGCC
GGAGCGTCATCTGCGACTCCAATGCCACTGCACTGGAGCTTCCCGGCCTT
CCTCTTTCCCTGCCCCAGCCCAGCATCCCCGCGGCTGTCCCGCAGAGTGC
TCCACCGGAGCCCCACCGGGAAGAGACCGTGACCGCCACCGCCACTTCCC
AGGTAGCCCAGCAGCCTCCAGCCGCTGCCGCCCCTGGGGAACAGGCCGTC
GCGGGCCCTGCCCCCTCGACTGTCCCCAGCAGTACCAGCAAAGACCGCCC
AGTGTCCCAGCCTAGCCTTGTGGGGAGCAAAGAGGAGCCGCCGCCGGCGA
GAAGTGGCAGCGGCGGCGGCAGCGCCAAGGAGCCACAGGAGGAACGGAGC
CAGCAGCAGGATGATATCGAAGAGCTGGAGACCAAGGCCGTGGGAATGTC
TAACGATGGCCGCTTTCTCAAGTTTGACATCGAAATCGGCAGAGGCTCCT
TTAAGACGGTCTACAAAGGTCTGGACACTGAAACCACCGTGGAAGTCGCC
TGGTGTGAACTGCAGGATCGAAAATTAACAAAGTCTGAGAGGCAGAGATT
TAAAGAAGAAGCTGAAATGTTAAAAGGTCTTCAGCATCCCAATATTGTTA
GATTTTATGATTCCTGGGAATCCACAGTAAAAGGAAAGAAGTGCATTGTT
TTGGTGACTGAACTTATGACGTCTGGAACACTTAAAACGTATCTGAAAAG
GTTTAAAGTGATGAAGATCAAAGTTCTAAGAAGCTGGTGCCGTCAGATCC
TTAAAGGTCTTCAGTTTCTTCATACTCGAACTCCACCTATCATTCACCGC
GATCTTAAATGTGACAACATCTTTATCACCGGCCCTACTGGCTCAGTCAA
GATTGGAGCCCTCGGTCTGGCAACCCTGAAGCGGGCTTCTTTTGCCAAGA
GTGTGATAGGTACCCCAGAGTTCATGGCCCCTGAGATGTATGAGGAGAAA
TATGATGAATCCGTTGACGTTTATGCTTTTGGGATGTGCATGCTTGAGAT
GGCTACATCTGAATATCCTTACTCGGAGTGCCAAAATGCTGCGCAGATCT
ACCGTCGCGTGACCAGTGGGGTGAAGCCAGCCAGTTTTGACAAAGTAGCA
ATTCCTGAAGTGAAGGAAATTATTGAAGGATGCATACGACAAAACAAAGA
TGAAAGATATTCCATCAAAGACCTTTTGAACCATGCCTTCTTCCAAGAGG
AAACAGGAGTACGGGTAGAATTAGCAGAAGAAGATGATGGAGAAAAAATA
GCCATAAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGGGAAA
ATACAAAGATAATGAAGCTATTGAGTTTTCTTTTGATTTAGAGAGAGATG
TCCCAGAAGATGTTGCACAAGAAATGGTAGAGTCTGGGTATGTCTGTGAA
GGTGATCACAAGACCATGGCTAAAGCTATCAAAGACAGAGTATCATTAAT
TAAGAGGAAACGAGAGCAGCGGCAGTTGGTACGGGAGGAGCAAGAAAAAA
AAAAGCAGGAAGAGAGCAGTCTCAAACAGCAGGTAGAACAATCCAGTGCT
TCCCAGACAGGAATCAAGCAGCTCCCTTCTGCTAGCACCGGCATACCTAC
TGCTTCTACCACTTCAGCTTCAGTTTCTACACAAGTAGAACCTGAAGAAC
CTGAGGCAGATCAACATCAGCAACTACAGTACCAGCAACCCAGTATATCT
GTGTTATCTGATGGGACGGTTGACAGTGGTCAGGGATCTTGACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSGGAAEKQSSTPGSLFLS
PPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAA
ATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEP
HREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQP
SLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGR
FLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEA
EMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVM
KIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGAL
GLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSE
YPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYS
IKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDN
EAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKR
EQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTT
SASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44447
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D368A; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.