cDNA Clone USP35

• Plasmid: pcDNA5D FRT/TO Halo Tev USP35 C450S (synth)
• Parent Plasmid: pcDNA5D FRT/TO HALO TEV
• Expressed: Halo-TEV-USP35 Cys450Ser
• Genbank: Q9P2H5
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGTCAGCGTGAAGCAGGGGCTGGTTCGGCGCGTGCTGGAGGCGGCGCGGCAGCCGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCGCGCCTCTACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCTGCACGTGGCCGGCCGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCGCGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGCGCCGGCCCCCCGGGCCCCCGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCCTGCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCGTGTGCGAGCGCCCGGGCCCCGCGGCCTGCGCGCAGGTGGCACGGCTGCTGGCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGCCTGCTCTTCTGCCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCGAGGAGGGCGCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTCCTGGCGCAGCTGTGGCGCGCACAGCCCGCCGCCATCCTGCCCTGCCTCAAAGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCTAGCGCCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTTGTCCGGAACCTCAGCAATGATGACAGTGTGACAGACTCGCAGATGCTGACTGCCATTAGCAGGATGATTGACTGGGTGTCCTGGCCCCTGGGGAAGAATATTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAGTTCAGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTCTAAGCTGCTGTACCCCATCGTCCGGGGAGCTGCCTTGTCTGTGCTCAAGTACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCACCTGCTCCTCCCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTCGGGGACCAGCTGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGTTCCGGTTCCCGGGCTTCCCGGATCTGTATGAGCCTGTCATGGAGGCCATCAAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGGCAGGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGCTCATGGCCAAGTCAGACACGGGCAAGATTGGTCTCATCAACCTGGGCAACACATGCTATGTCAACAGCATCCTTCAGGCCTTATTCATGGCGTCTGACTTCAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGACCAAGCTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCCATTTCCCCAGAGAACTTCCTCTCCGCATCCTGGACGCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTGGATCGGCTGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAGTCCAGCTCGCCCTCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTCTGTGGAAAAAATGTTTGGAGGCAAGATAGTGACTCGGATCTGCTGTCTCTGCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTCGCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGATGCGCCCCACAGAAGACATCACAGCCCGGGAGTTGCCCCCACCAACCAGTGCACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGGAAACACTGCATCACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAAGGAAGCTGGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAGAAGGGAAGGAGGAGAGAACGGAGAAGGAAGAAGTGGGGGAGGAGGAGGAAAGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAAGAGGAAGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGGAAGAAGACAGCCTGGGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCCGGGGAGCGGACATGTGGCTCTGAGGGCTCCCGCTCCGTCCTGGACCTGGTTAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACTGCGAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGCCAAGGGCCGTGCTACCTCATCCTCACACTGCTGCGCTTCTCTTTCGACCTGCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCCATCCCCCTGCTGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGCAGTGTGGTGGTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTGCTATGCCCGTGAGGGCGCTGCCCGCCCTGCCGCTTCTCTGGGAACTGCCGATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGGGTGTCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAAGGACACAGCCTATGTGCTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCGAGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAGCCCACCCTGCACAAGGACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCAGGAGAAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACATCTCCGCACTGGGGGAGGGGCTTTGATGAAGACAAGGATGAGGATGAAGGCTCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGACTTCCACAGACTGGTCTTCTAAGCGGCCGC

Amino Acid Sequence

ASATMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALG
LEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFAR
ETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNP
VDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVL
IPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEIS
GENLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQCLA
LLALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQ
GGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAA
CAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLE
QAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHL
PLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALL
KGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHS
HEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDL
YEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGK
IGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGF
LEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGT
RICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSRE
EAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGP
RRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEK
EEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGT
HPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQD
AEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGG
RGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQW
YLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRV
RTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFD
EDKDEDEGSPGGCNPAGGNGGDFHRLVF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25134
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sub-cloned from Synthesised clone; Parental Plasmid DU23534