cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pcDNA5frtTO GFP TTBK2 bam not
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-TTBK2
• Genbank: NM_173500.3
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGTGGGGGAGGAGAGCAGCCGGATATCCTGAGTGTTGG
AATCCTAGTGAAAGAAAGATGGAAAGTGTTGAGAAAGATTGGGGGTGGGG
GCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAAAATGTT
GCACTGAAGGTGGAATCAGCTCAACAACCAAAACAAGTTCTGAAAATGGA
AGTTGCTGTTTTGAAAAAGCTGCAAGGGAAAGACCATGTTTGTAGATTTA
TTGGCTGTGGGAGGAATGATCGATTCAACTATGTGGTCATGCAGTTGCAG
GGTCGGAATCTGGCAGATCTTCGCCGTAGCCAGTCCCGAGGCACATTCAC
CATTAGTACCACTCTCCGGCTGGGTAGACAGATTTTGGAGTCTATTGAAA
GCATTCATTCTGTGGGATTCTTGCATCGAGACATCAAACCGTCGAACTTC
GCTATGGGTCGCTTTCCTAGTACATGTAGGAAATGTTACATGCTTGATTT
TGGCTTGGCTCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTC
GAGCTGTGGCAGGTTTTCGAGGGACAGTTCGTTATGCATCAATCAACGCA
CATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCCTTATTCTA
CATGTTGGTGGAGTTTGTGGTTGGTCAGCTGCCCTGGAGAAAAATAAAGG
ACAAGGAGCAAGTAGGCTCTATTAAGGAGAGATATGACCACAGGCTCATG
TTGAAACATCTCCCTCCAGAATTCAGCATCTTTCTAGACCATATCTCTTC
TTTGGATTATTTTACAAAACCAGACTACCAGCTTCTTACATCCGTGTTTG
ACAATAGCATCAAGACTTTTGGAGTAATTGAGAGTGACCCTTTTGACTGG
GAGAAGACTGGAAATGATGGCTCCCTAACAACCACCACTACTTCTACCAC
CCCTCAGTTGCACACTCGCTTGACCCCTGCTGCAATTGGAATTGCCAATG
CTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATACAGATGAGGTATTT
CCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCCCTGTTGGTGTGTC
ACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGTCCCCAGGAGAAGG
ATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGATAAAGCTTGGAATT
TGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCCAGGCAAATGGTCT
TCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTCCGCTCAGAGATTA
CTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTTACGTTCCATTCAC
AGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAAAGCCAGACACTGA
CAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAAGATACCAGTGCAG
GAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCCTTGCGTTCCTGCT
GTGTCCCGTACTGACCACATCTGGCACTATGATGAAGAATATCTTCCAGA
TGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAGGCAGATGGTGGTG
GCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTGCAAGCAAGAAATT
GATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGGACCTTCAGGATTT
TAGGACAAATGAGGCTGTAGGACATAAAACAACTGGAAGTCCTTCTGATG
AGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACCTCAAGATGAAAAG
CTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTC
AGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAG
AACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTT
ATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCT
TACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTT
CAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCC
ATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTT
GGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGG
GGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAA
AGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAG
ACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAA
GCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAG
CATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAA
GGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTT
TTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTG
ACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGA
AGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGG
ATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCT
ACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGG
AGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGA
AGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTT
TTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGC
AAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGC
TTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGT
GCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTT
TTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTC
ACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAG
TCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCA
GGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAA
AGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTC
CTAGGGAGTAGTAACTCCGACTCAGACCTTTTCTCCCGCCTGGCCCAAAT
TCTTCAAAATGGATCTCAGAAACCCCGGAGCACTACTCAGTGCAAGAGTC
CAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTCCCT
CGCAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGCACGTC
TAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTT
CGTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCT
TCCTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAA
TGGCCTGAAAGGCTCCGGCAGCCTCCACCACCACTCAGCCAGCACTAAAA
CCCCCCAAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGATAGCGGCCG
C

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLAAAMSGGG
EQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQ
QPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLR
RSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPST
CRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGR
HDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEF
SIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKTGNDGS
LTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSDG
ENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDANKNKIKLGICKAATEEE
NSHGQANGLLNAPSLGSPIRVRSEITQPDRDIPLVRKLRSIHSFELEKRL
TLEPKPDTDKFLETCLEKMQKDTSAGKESILPALLHKPCVPAVSRTDHIW
HYDEEYLPDASKPASANTPEQADGGGSNGFIAVNLSSCKQEIDSKEWVIV
DKEQDLQDFRTNEAVGHKTTGSPSDEEPEVLQVLEASPQDEKLQLGPWAE
NDHLKKETSGVVLALSAEGPPTAASEQYTDRLELQPGAASQFIAATPTSL
MEAQAEGPLTAITIPRPSVASTQSTSGSFHCGQQPEKKDLQPMEPTVELY
SPRENFSGLVVTEGEPPSGGSRTDLGLQIDHIGHDMLPNIRESNKSQDLG
PKELPDHNRLVVREFENLPGETEEKSILLESDNEDEKLSRGQHCIEISSL
PGDLVIVEKDHSATTEPLDVTKTQTFSVVPNQDKNNEIMKLLTVGTSEIS
SRDIDPHVEGQIGQVAEMQKNKISKDDDIMSEDLPGHQGDLSTFLHQEGK
REKITPRNGELFHCVSENEHGAPTRKDMVRSSFVTRHSRIPVLAQEIDST
LESSSPVSAKEKLLQKKAYQPDLVKLLVEKRQFKSFLGDLSSASDKLLEE
KLATVPAPFCEEEVLTPFSRLTVDSHLSRSAEDSFLSPIISQSRKSKIPR
PVSWVNTDQVNSSTSSQFFPRPPPGKPPTRPGVEARLRRYKVLGSSNSDS
DLFSRLAQILQNGSQKPRSTTQCKSPGSPHNPKTPPKSPVVPRRSPSASP
RSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPPSHSGSSSSRRSCQ
QEHCKPSKNGLKGSGSLHHHSASTKTPQGKSKPASKLSR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44453
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Internal BamHI site KO'd, this fragment may be excised BamHI/NotI for directional downstream cloning work.