cDNA Clone ULK1

• Synonyms: ATG1, ATG1A, hATG1, UNC51, Unc51.1
• Plasmid: pEBG6P-1 ULK1 M92T
• Parent Plasmid: pEBG6P
• Expressed: GST-ULK1 M92T
• Genbank: NM_003565.2 GI:225637564 with silent changes c1065t, c1779g and coding change c2141t (P714L (exisiting SNP)
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCTGGAGCCCGGCCGCGGCGGCACAGAGACCGTGGGCAAGTTCGAGTTCTCCCGCAAGGACCTGATCGGCCACGGCGCCTTCGCGGTGGTCTTCAAGGGCCGCCACCGCGAGAAGCACGATTTGGAGGTCGCCGTCAAGTGCATTAACAAGAAGAACCTCGCCAAGTCTCAGACGCTGCTGGGGAAGGAAATCAAAATCCTGAAGGAACTGAAACATGAAAACATCGTGGCCCTGTACGACTTCCAGGAAATGGCTAATTCTGTCTACCTGGTTACCGAGTACTGCAACGGTGGGGACCTGGCCGACTACCTGCACGCCATGCGCACGCTGAGCGAGGACACCATCAGGCTCTTCCTGCAGCAGATCGCGGGCGCCATGCGGCTTCTGCACAGCAAAGGCATCATCCACCGCGACCTGAAACCGCAGAACATCCTGCTGTCCAACCCCGCCGGCCGCCGCGCCAACCCCAACAGCATCCGCGTCAAGATCGCTGACTTCGGCTTCGCGCGGTACCTCCAGAGCAACATGATGGCGGCCACACTCTGCGGCTCCCCCATGTACATGGCCCCCGAGGTCATCATGTCCCAGCACTACGACGGGAAGGCGGACCTGTGGAGCATCGGCACCATCGTCTACCAGTGCCTGACGGGGAAGGCGCCCTTCCAGGCCAGCAGCCCCCAGGACCTGCGCCTGTTCTACGAGAAGAACAAGACGTTGGTCCCCACCATCCCCCGGGAGACCTCGGCCCCGCTGCGGCAGCTGCTCCTGGCCCTACTGCAACGCAACCACAAGGACCGCATGGACTTCGATGAGTTTTTTCATCACCCTTTCCTCGATGCCAGCCCCTCGGTCAGGAAATCCCCACCCGTGCCTGTGCCCTCGTACCCAAGCTCGGGGTCCGGCAGCAGCTCCAGCAGCAGCTCCACCTCCCACCTGGCCTCCCCGCCGTCCCTGGGCGAGATGCAGCAGCTGCAGAAGACCCTGGCCTCCCCGGCTGACACCGCTGGCTTCCTGCACAGCTCCCGGGACTCTGGTGGCAGCAAGGACTCTTCCTGTGACACAGATGACTTCGTCATGGTCCCCGCGCAGTTTCCAGGTGACCTGGTGGCTGAGGCGCCCAGTGCCAAACCCCCGCCAGACAGCCTGATGTGCAGTGGGAGCTCACTGGTGGCCTCTGCGGGCTTGGAGAGCCACGGCCGGACCCCATCTCCATCCCCACCCTGCAGCAGCTCCCCCAGTCCCTCAGGCCGGGCTGGCCCGTTCTCCAGCAGCAGGTGCGGCGCCTCTGTCCCCATCCCAGTCCCCACGCAGGTGCAGAACTACCAGCGCATTGAGCGAAACCTGCAGTCACCCACCCAGTTCCAAACACCTCGGTCCTCTGCCATCCGCAGGTCAGGCAGCACCAGCCCCCTGGGCTTTGCAAGGGCCAGCCCCTCGCCCCCTGCCCACGCTGAGCATGGAGGCGTCCTGGCCAGGAAGATGTCTCTGGGTGGAGGCCGGCCCTACACGCCATCTCCTCAAGTTGGAACCATCCCTGAGCGGCCAGGCTGGAGCGGGACGCCCTCCCCACAGGGAGCTGAGATGCGGGGTGGCAGGTCCCCTCGTCCAGGCTCCTCTGCACCCGAGCACTCTCCCCGCACTTCCGGGCTGGGCTGCCGCCTGCACAGCGCCCCCAACCTGTCTGACTTGCACGTCGTCCGCCCCAAGCTGCCCAAACCCCCCACGGACCCCCTGGGAGCTGTGTTCAGCCCACCACAGGCCAGCCCTCCCCAGCCGTCGCACGGCCTGCAGTCCTGCCGGAACCTGCGGGGCTCACCCAAGCTGCCCGACTTCCTGCAGCGAAACCCCCTGCCCCCCATCCTGGGCTCCCCCACCAAGGCTGTGCCCTCCTTTGACTTCCCGAAGACCCCCAGCTCCCAGAACCTGCTGGCCCTCCTAGCCCGGCAGGGCGTGGTGATGACGCCCCCTCGAAACCGGACGCTGCCCGACCTCTCGGAGGTGGGACCCTTCCATGGTCAGCCGTTGGGCCCTGGCCTGCGGCCAGGCGAGGACCCCAAGGGCCCCTTTGGCCGGTCTTTCAGCACCAGCCGCCTCACTGACCTGCTCCTTAAGGCGGCGTTTGGGACACAAGCCCCGGACCTGGGCAGCACGGAGAGCCTGCAGGAGAAGCCCATGGAGATCGCACCCTCAGCTGGCTTTGGAGGGAGCCTGCACCCAGGAGCCCGTGCTGGGGGCACCAGCAGCCCTTCCCCGGTGGTCTTCACCGTGGGCTCTCCCCCGAGCGGGAGCACGCCCCCCCAGGGCCCCCGCACCAGGATGTTCTCAGCGGGCCCCACTGGCTCTGCCAGCTCTTCTGCCCGCCACCTGGTGCCTGGGCCCTGCAGCGAGGCCCCAGCCCCTGAGCTCCCTGCTCCAGGACACGGCTGCAGCTTTGCCGACCCCATTGCTGCGAACCTGGAGGGGGCTGTGACCTTCGAGGCCCCCGACCTCCCTGAGGAGACCCTCATGGAGCAAGAGCACACGGAGATCCTGCGTGGCCTGCGCTTCACGCTGCTGTTCGTGCAGCACGTCCTGGAGATCGCAGCCCTGAAGGGCAGCGCCAGTGAGGCGGCGGGGGGCCCTGAGTACCAGCTGCAGGAGAGTGTGGTGGCCGACCAGATCAGCCTGCTGAGCCGAGAATGGGGCTTCGCGGAACAGCTGGTGCTGTACCTGAAGGTGGCCGAGCTACTGTCCTCCGGCCTGCAAAGTGCCATCGACCAGATCCGGGCCGGCAAGCTCTGCCTGTCGTCCACTGTGAAGCAGGTGGTGCGCAGGCTGAATGAGCTGTACAAGGCCAGCGTGGTGTCCTGCCAGGGCCTGAGCCTGCGGCTGCAGCGCTTCTTCCTGGACAAGCAGCGGCTCCTGGACCGCATTCACAGCATCACTGCCGAGAGGCTCATCTTCAGCCACGCTGTGCAGATGGTGCAGTCGGCTGCCCTGGACGAGATGTTCCAGCACCGTGAGGGCTGCGTCCCACGCTACCACAAGGCCCTGCTGCTCCTGGAGGGGCTGCAGCACATGCTCTCGGACCAGGCCGACATCGAGAACGTCACCAAGTGCAAGCTGTGCATTGAGCGGAGACTCTCGGCGCTGCTGACTGGCATCTGTGCCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSLEPGRGGTETVGK
FEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKE
IKILKELKHENIVALYDFQEMANSVYLVTEYCNGGDLADYLHAMRTLSED
TIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKI
ADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQ
CLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNH
KDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLA
SPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQ
FPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPS
PSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIR
RSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPE
RPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDL
HVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQ
RNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPD
LSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPD
LGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGS
TPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFAD
PIAANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAAL
KGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSG
LQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLD
KQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLL
LEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45449
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Grow at 30 degrees.