cDNA Clone SLX4

• Synonyms: BTBD12
• Plasmid: pET28a 6His-TEV(not blunt)-Halo 4xSLX4 270-380 C296/299A
• Parent Plasmid: pET28a 6His TEV Halo
• Expressed: 6His-TEV(not blunt)-Halo 4xSLX4 270-380 C296/299A
• Genbank: NM_032444.2 GI:63252862
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCATCGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCGCCCAGATTGCTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGTGAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTCAGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGCAGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCAGCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGATCCATGTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCATCGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCGCCCAGATTGCTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGTGAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTCAGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGCAGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCAGCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGATCCATGTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCATCGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCGCCCAGATTGCTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGTGAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTCAGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGCAGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCAGCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGATCCATGTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCATCGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCGCCCAGATTGCTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGTGAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTCAGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGCAGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCAGCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGATCTTGACATCGAGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHENLYFQGGMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDG
TPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFD
DHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFI
RPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLT
EVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQS
PVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
GSEIARWLSTLEISGGSMLTLQQEFARVGASAHDDSLEEKGLFFAQIAQK
NLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTS
HLKQCAVKMEVGPQLLLQAVRLQTAQPEGRSMLTLQQEFARVGASAHDDS
LEEKGLFFAQIAQKNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECP
ICGKPFLTLKSRTSHLKQCAVKMEVGPQLLLQAVRLQTAQPEGRSMLTLQ
QEFARVGASAHDDSLEEKGLFFAQIAQKNLSAMNVTRREQHVNRCLDEAE
KTLRPSVPQIPECPICGKPFLTLKSRTSHLKQCAVKMEVGPQLLLQAVRL
QTAQPEGRSMLTLQQEFARVGASAHDDSLEEKGLFFAQIAQKNLSAMNVT
RREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQCAVK
MEVGPQLLLQAVRLQTAQPEGRS*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU45462
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Parental 23918