cDNA Clone SGK3

• Synonyms: CISK, SGKL
• Plasmid: pcDNA5frtTO HA SGK3 K191A shRNA.B prot
• Parent Plasmid: pcDNA5D FRT/TO HA
• Expressed: HA-SGK3 K191A shRNA.B prot
• Genbank: NM_013257.4
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCAAAGAGATCACACCATGGACTACAAGGAAAGCTGCCCAAGTGTAAGCATTCCCAGCTCCGATGAACACAGAGAGAAAAAGAAGAGGTTTACTGTTTATAAAGTTCTGGTTTCAGTGGGAAGAAGTGAATGGTTTGTCTTCAGGAGATATGCAGAGTTTGATAAACTTTATAACACTTTAAAAAAACAGTTTCCTGCTATGGCCCTGAAGATTCCTGCCAAGAGAATATTTGGTGATAATTTTGATCCAGATTTTATTAAACAAAGACGAGCGGGCCTCAACGAATTCATTCAGAACCTAGTTAGGTATCCAGAACTTTATAACCATCCAGATGTCAGAGCATTCCTTCAAATGGACAGTCCAAAACACCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTTCTCAGAAGCTACACTCTACCTCACAGAACATCAACCTGGGACCGTCTGGAAATCCTCATGCCAAACCAACTGACTTTGATTTCTTAAAAGTTATTGGAAAAGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGATGGAAAATTTTATGCTGTCGCAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATTATGGCTGAACGTAATGTGCTCTTGAAAAATGTGAAACATCCGTTTTTGGTTGGATTGCATTATTCCTTCCAAACAACTGAAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTTTTTTTCCACTTACAAAGAGAACGGTCCTTTCCTGAGCACAGAGCTAGGTTTTACGCTGCTGAAATTGCTAGTGCATTGGGTTACTTACATTCCATCAAAATAGTATACAGAGACTTGAAACCAGAAAATATTCTTTTGGATTCAGTAGGACATGTTGTCTTAACAGATTTTGGGCTTTGTAAAGAAGGAATTGCTATTTCTGACACCACTACCACATTTTGTGGGACACCAGAGTATCTTGCACCTGAAGTAATTAGAAAACAGCCCTATGACAATACTGTAGATTGGTGGTGCCTTGGGGCTGTTCTGTATGAAATGCTGTATGGATTGCCTCCTTTTTATTGCCGAGATGTTGCTGAAATGTATGACAATATCCTTCACAAACCCCTAAGTTTGAGGCCAGGAGTGAGTCTTACAGCCTGGTCCATTCTGGAAGAACTCCTAGAAAAAGACAGGCAAAATCGACTTGGTGCCAAGGAAGACTTTCTTGAAATTCAGAATCATCCTTTTTTTGAATCACTCAGCTGGGCTGACCTTGTACAAAAGAAGATTCCACCACCATTTAATCCTAATGTGGCTGGACCAGATGATATCAGAAACTTTGACACAGCATTTACAGAAGAAACAGTTCCATATTCTGTGTGTGTATCTTCTGACTATTCTATAGTGAATGCCAGTGTATTGGAGGCAGATGATGCATTCGTTGGTTTCTCTTATGCACCTCCTTCAGAAGACTTATTTTTGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMQRDHTMDYKESCPSVSIPSSDEHREKKKRFTVYKVLV
SVGRSEWFVFRRYAEFDKLYNTLKKQFPAMALKIPAKRIFGDNFDPDFIK
QRRAGLNEFIQNLVRYPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSSQ
KLHSTSQNINLGPSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFY
AVAVLQKKIVLNRKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYF
VLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLK
PENILLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPY
DNTVDWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTA
WSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNP
NVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYA
PPSEDLFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44509
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: K191A and silent mutations to protect against shRNA.B