cDNA Clone SIK2

• Synonyms: QIK
• Plasmid: pRetrox tight HA SIK2 S343A
• Parent Plasmid: pRetroX tight puro
• Expressed: HA SIK2 S343A
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCGGCCgccgccaccatgTACCCATACGATGTGCCAGATTACGC
CGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCGGTCCGGGTGG
GGTTCTACGACATCGAGGGCACGCTGGGCAAGGGCAACTTCGCTGTGGTG
AAGCTGGGGCGGCACCGGATCACCAAGACGGAGGTGGCAATAAAAATAAT
CGATAAGTCTCAGCTGGATGCAGTGAACCTTGAGAAAATCTACCGAGAAG
TACAAATAATGAAAATGTTAGACCACCCTCACATAATCAAACTTTATCAG
GTAATGGAGACCAAAAGTATGTTGTACCTTGTGACAGAATATGCCAAAAA
TGGAGAAATTTTTGACTATCTTGCTAATCATGGCCGGTTAAATGAGTCTG
AAGCCAGGCGAAAATTCTGGCAAATCCTGTCTGCTGTTGATTATTGTCAT
GGTCGGAAGATTGTGCACCGTGACCTCAAAGCTGAAAATCTCCTGCTGGA
TAACAACATGAATATCAAAATAGCAGATTTCGGTTTTGGAAATTTCTTTA
AAAGTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCCCCTTATGCAGCC
CCAGAAGTCTTTGAAGGGCAGCAGTATGAAGGACCACAGCTGGACATCTG
GAGTATGGGAGTTGTTCTTTATGTCCTTGTCTGTGGAGCTCTGCCCTTTG
ATGGACCGACTCTTCCAATTTTGAGGCAGAGGGTTCTGGAAGGAAGATTC
CGGATTCCGTATTTCATGTCAGAAGATTGCGAGCACCTTATCCGAAGGAT
GTTGGTCCTAGACCCATCCAAACGGCTAACCATAGCCCAAATCAAGGAGC
ATAAATGGATGCTCATAGAAGTTCCTGTCCAGAGACCTGTTCTCTATCCA
CAAGAGCAAGAAAATGAGCCATCCATCGGGGAGTTTAATGAGCAGGTTCT
GCGACTGATGCACAGCCTTGGAATAGATCAGCAGAAAACCATTGAGTCTT
TGCAGAACAAGAGCTATAACCACTTTGCTGCCATTTATTTCTTGTTGGTG
GAGCGCCTGAAATCACATCGGAGCgcTTTCCCAGTGGAGCAGAGACTTGA
TGGCCGCCAGCGTCGGCCTAGCACCATTGCTGAGCAAACAGTTGCCAAGG
CACAGACTGTGGGGCTCCCAGTGACCATGCATTCACCGAACATGAGGCTG
CTGCGATCTGCCCTCCTCCCCCAGGCATCCAACGTGGAGGCCTTTTCATT
TCCAGCATCTGGCTGTCAGGCGGAAGCTGCATTCATGGAAGAAGAGTGTG
TGGACACTCCAAAGGTCAATGGCTGTCTGCTTGACCCTGTGCCTCCTGTC
CTGGTGCGGAAGGGATGCCAGTCACTGCCCAGCAACATGATGGAGACCTC
CATTGACGAAGGGCTGGAGACAGAAGGAGAGGCCGAGGAAGACCCCGCTC
ATGCCTTTGAGGCATTTCAGTCCACACGCAGCGGGCAGAGACGGCACACT
CTGTCAGAAGTGACCAATCAACTGGTCGTGATGCCTGGGGCAGGGAAAAT
TTTCTCCATGAATGACAGCCCCTCCCTTGACAGTGTGGACTCTGAGTATG
ATATGGGGTCTGTTCAGAGGGACCTGAACTTTCTGGAAGACAACCCTTCC
CTTAAGGACATCATGTTAGCCAATCAGCCTTCACCCCGCATGACATCTCC
CTTCATAAGCCTGAGACCTACCAACCCAGCCATGCAGGCTCTGAGCTCCC
AGAAACGAGAGGTCCACAACAGGTCTCCAGTGAGCTTCAGAGAGGGCCGC
AGAGCATCAGATACCTCCCTCACCCAGGGAATTGTAGCATTTAGACAACA
TCTTCAGAATCTGGCTAGAACCAAAGGAATTCTAGAGTTGAACAAAGTGC
AGTTGTTGTATGAACAAATAGGACCGGAGGCAGACCCTAACCTGGCGCCG
GCGGCTCCTCAGCTCCAGGACCTTGCTAGCAGCTGCCCTCAGGAAGAAGT
TTCTCAGCAGCAGGAAAGCGTCTCCACTCTCCCTGCCAGCGTGCATCCCC
AGCTGTCCCCACGGCAGAGCCTGGAGACCCAGTACCTGCAGCACAGACTC
CAGAAGCCCAGCCTTCTGTCAAAGGCCCAGAACACCTGTCAGCTTTATTG
CAAAGAACCACCGCGGAGCCTTGAGCAGCAGCTGCAGGAACATAGGCTCC
AGCAGAAGCGACTCTTTCTTCAGAAGCAGTCTCAACTGCAGGCCTATTTT
AATCAGATGCAGATAGCAGAGAGCTCCTACCCACAGCCAAGTCAGCAGCT
GCCCCTTCCCCGCCAGGAGACTCCACCGCCTTCTCAGCAGGCCCCACCGT
TCAGCCTGACCCAGCCCCTGAGCCCCGTCCTGGAGCCTTCCTCCGAGCAG
ATGCAATACAGCCCTTTCCTCAGCCAGTACCAAGAGATGCAGCTTCAGCC
CCTGCCCTCCACTTCCGGTCCCCGGGCTGCTCCTCCTCTGCCCACGCAGC
TACAGCAGCAGCAGCCGCCACCGCCACCACCCCCTCCACCACCACGACAG
CCAGGAGCTGCCCCAGCCCCCTTACAGTTCTCCTATCAGACTTGTGAGCT
GCCAAGCGCTGCTTCCCCTGCGCCAGACTATCCCACTCCCTGTCAGTATC
CTGTGGATGGAGCCCAGCAGAGCGACCTAACGGGGCCAGACTGTCCCAGA
AGCCCAGGACTGCAAGAGGCCCCCTCCAGCTACGACCCACTAGCCCTCTC
TGAGCTACCTGGACTCTTTGATTGTGAAATGCTAGACGCTGTGGATCCAC
AACACAACGGGTATGTCCTGGTGAATTAgcggccgc

Amino Acid Sequence

MYPYDVPDYAVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRI
TKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKSM
LYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVHR
DLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQ
QYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFMS
EDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEP
SIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHR
SAFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLP
QASNVEAFSFPASGCQAEAAFMEEECVDTPKVNGCLLDPVPPVLVRKGCQ
SLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTNQ
LVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLA
NQPSPRMTSPFISLRPTNPAMQALSSQKREVHNRSPVSFREGRRASDTSL
TQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADPNLAPAAPQLQD
LASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLS
KAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAE
SSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVLEPSSEQMQYSPFL
SQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPAP
LQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEA
PSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU43767
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee