cDNA Clone TJP1

• Synonyms: ZO-1
• Plasmid: pcDNA5frtTO HA TJP1
• Parent Plasmid: pcDNA5D FRT/TO HA
• Expressed: HA-TJP1
• Genbank: NM_003257.3
• Tag: HA
• Species: Human

Sequence of Insert

gtcgacATGTCCGCCAGAGCTGCAGCCGCCAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAACATACAGTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGTGGACGAGATAATCCTCATTTTCAGAGTGGGGAAACGTCAATAGTGATTTCAGATGTGCTGAAAGGAGGACCAGCTGAAGGACAGCTACAGGAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTTTTGCTGTTCAGCAACTAAGGAAAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCAAATACCAGTAAGTCGTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATACATGATCCAAGAAGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAAGTGCAAGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAGCCTGCTAAACCTACTAAAGTCACACTGGTGAAATCCCGGAAAAATGAAGAATATGGTCTTCGATTGGCAAGCCATATATTTGTTAAGGAAATTTCACAAGATAGTTTGGCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTATTGAAGATAAATGGTACTGTGACAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAAAGGCAAATTAAAAATGGTAGTTCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGACAGCATCCACTCTGCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCATTCTGGTCGATCACACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCAGAGCCTTCTGATCATTCCAGGCACTCGCCGCAGCAGCCAAGCAATGGCAGTCTCCGGAGTAGAGATGAAGAGAGAATTTCTAAACCTGGGGCTGTCTCAACTCCTGTAAAGCATGCTGATGATCACACACCTAAAACAGTGGAAGAAGTTACAGTTGAAAGAAATGAGAAACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGGGCAACCAGATGTGGATTTACCTGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGGATTCTTCGGCCCAGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTGGCTGGTGGAAATGATGTTGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGCTTAGAGGAAGGTGATCAAATTCTCAGGGTAAACAACGTAGATTTTACAAATATCATAAGAGAAGAAGCCGTCCTTTTCCTGCTTGACCTCCCTAAAGGAGAAGAAGTGACCATATTGGCTCAGAAGAAGAAGGATGTTTATCGTCGCATTGTAGAATCAGATGTAGGAGATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTATGGACTTAGTTTTAACAAAGGAGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCTTGGCTTGCTATTCGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAGCAGCTAGCCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGAGACCGTGCTGACTTCTGGAGATTCAGAGGTCTTCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGATTTGTCCGCTCAGCCTGTTCAAACAAAGTTTCCAGCTTATGAAAGAGTGGTTCTTCGAGAAGCTGGATTTCTGAGGCCTGTAACCATTTTTGGACCAATAGCTGATGTTGCCAGAGAAAAGCTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAAGAGTGAACCACGAGACGCTGGAACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAAATCATAGATCAAGACAAACATGCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTTAACTATGCCCAGTGGTATCCAATTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTAAAAACAATGAGAATGAGGTTATGTCCAGAATCTCGGAAAAGTGCCAGGAAGTTATACGAGCGATCTCATAAACTTCGTAAAAATAATCACCATCTTTTTACAACTACAATTAACTTAAATTCAATGAATGATGGTTGGTATGGTGCGCTGAAAGAAGCAATTCAACAACAGCAAAACCAGCTGGTATGGGTTTCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGATGATCGTCTGTCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGACAGTAGACACACTTCTGACTATGAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATGATGAGGTTGGGACTCCACCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGACTCCTCTGGAATGCATCATGAAAACCAAACATATCCTCCTTACTCACCACAAGCGCAGCCACAACCAATTCATAGAATAGACTCCCCTGGATTTAAGCCAGCCTCTCAACAGAAAGCAGAAGCTTCATCTCCAGTCCCTTACCTTTCGCCTGAAACAAACCCAGCATCATCAACCTCTGCTGTTAATCATAATGTAAATTTAACTAATGTCAGACTGGAGGAGCCCACCCCAGCTCCTTCCACCTCTTACTCACCACAAGCTGATTCTTTAAGAACACCAAGTACTGAGGCAGCTCACATAATGCTAAGAGATCAAGAACCATCATTGTCGTCGCATGTAGATCCAACAAAGGTGTATAGAAAGGATCCATATCCCGAGGAAATGATGAGGCAGAACCATGTTTTGAAACAGCCAGCCGTTAGTCACCCAGGGCACAGGCCAGACAAAGAGCCTAATCTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCCAGCAGAGACCTCGAGCAGCCCACATACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACTATGAACATCGTCTGCGATACGAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAAACAGCCCTACCCATCTCGGCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCCGAAGAGTCCTCAGAACGAGGGTACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTACGACAGCAGACCACGTTACGAACAGGCACCTAGAGCATCCGCCCTGCGGCACGAAGAGCAGCCAGCTCCTGGGTATGACACACATGGTAGACTCAGACCGGAAGCCCAGCCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAGTATTTTGAGCAATATTCACGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATTTTGAGCCTCTCCATGGTGCTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCTGCCTTCAAACACCAAACCACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAGCCACAGTCTGTACTCACCAGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAGACCAAGAAGGATGTAAATGACACTGGCAGTTTTAAGCCTCCAGAAGTAGCATCTAAACCTTCAGGTGCTCCCATCATTGGTCCCAAACCCACTTCTCAGAATCAATTCAGTGAACATGACAAAACTCTGTACAGGATCCCAGAACCTCAAAAACCTCAACTGAAGCCACCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAATATTATCGAAAACAGCTGTCATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCTGCCAGCCATCTCTCCGAGCCTGCAAAGCCAGCTCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCAAAGGGAAAGCCTCCTGAAGCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAACCCATCCAGGCCACTCCCCCTCCTCCTCCATTGCCCTCGCAGTATGCCCAGCCATCTCAGCCTGTCACCAGCGCGTCTCTCCACATACATTCTAAGGGAGCACATGGTGAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTGTCCAAACCAGACCCACCTCCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTGCTCAGGCAGCTTTCTATCCCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCAGAAAACAGTCACTCCAGCATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAACGCAAGTTTGAAAGTCCTAAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAACCTGACTTGTCTTCAAAAACTCCCACTTCTCCAAAAACTCTTGTGAAATCGCACAGTTTGGCACAGCCTCCTGAGTTTGACAGTGGAGTTGAAACTTTCTCTATCCATGCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTGCCTAAAGCTATTCCTGTGAGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGGCCACAGCCCGAGGCATATTTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATAATTATCCCTCAAGGAGCCATTCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAACAGCATCCTTCCACCTTTAGATAAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGTGGTCCCCATGGCCTCAAGTTCCTGAAGCCTGTGGAGCTGCGCTTACCACACTGTGATCCTAAAACCTGGCAAAACAAGTGTCTTCCCGGAGATCCAAATTATCTCGTTGGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTTTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSPGEFASLDMSARAAAAKSTAMEETAIWEQHTVTLHRAP
GFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNG
VSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEED
SYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQ
PAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVL
KINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHS
ANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSP
QQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTP
SLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKG
DSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIR
EEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKE
SPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRA
EQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKF
PAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAG
TDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNP
DSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMND
GWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEY
SMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEP
VREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVP
YLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTE
AAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHR
PDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRY
EDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFP
RFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPS
AGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSS
QHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLE
TKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQ
KPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHL
SEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPP
LPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQN
KPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPK
PYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSL
AQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGH
TVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNS
ILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPG
DPNYLVGANCVSVLIDHF*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44557
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee