cDNA Clone TRPM6

• Synonyms: CHAK2, HMGX, HOMG, HOMG1, HSH
• Plasmid: pcDNA5frtTO FLAG TRPM6 1-1700
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG-TRPM6 1-1700
• Genbank: NM_017662.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGAAAGAACAACCTGTCTTGGAGCGCTTGCAGTCCCAGAAATC
CTGGATTAAAGGAGTATTTGACAAGAGAGAATGTAGCACAATCATACCCA
GCTCAAAAAATCCTCACAGATGTACTCCAGTATGCCAAGTCTGCCAGAAT
TTAATCAGGTGTTACTGTGGCCGACTGATTGGAGACCATGCTGGGATAGA
TTATTCCTGGACCATCTCAGCTGCCAAGGGTAAAGAAAGTGAACAATGGT
CTGTTGAAAAGCACACAACGAAAAGCCCAACAGATACTTTTGGCACGATT
AATTTCCAAGATGGAGAGCACACCCATCATGCCAAGTATATTAGAACTTC
TTATGATACAAAACTGGATCATCTGTTACATTTAATGTTGAAAGAGTGGA
AAATGGAACTGCCCAAGCTTGTGATCTCAGTCCATGGGGGCATCCAGAAC
TTTACTATGCCCTCTAAATTTAAAGAGATTTTCAGCCAAGGTTTGGTTAA
AGCTGCAGAGACAACCGGTGCGTGGATAATAACTGAAGGCATCAATACAG
GAGTGTCCAAGCATGTTGGGGATGCCTTGAAATCCCATTCCTCTCATTCC
TTGAGAAAAATCTGGACAGTTGGAATCCCTCCTTGGGGTGTCATTGAGAA
CCAGAGAGACCTTATTGGAAAAGATGTGGTGTGCCTGTACCAGACTCTGG
ATAACCCCCTCAGCAAGCTCACAACACTCAACAGCATGCACTCGCACTTC
ATCCTGTCTGATGATGGGACCGTGGGCAAGTATGGAAATGAAATGAAGCT
CAGAAGGAACCTGGAGAAGTACCTCTCTCTGCAGAAAATACACTGCCGCT
CAAGACAAGGCGTGCCGGTCGTGGGGCTGGTGGTGGAAGGCGGTCCCAAC
GTCATCCTGTCAGTGTGGGAGACTGTCAAGGACAAGGACCCAGTGGTGGT
GTGTGAGGGTACAGGTAGGGCGGCTGACCTCCTGGCCTTCACACACAAAC
ACCTGGCAGATGAAGGGATGCTGCGACCTCAGGTGAAAGAGGAGATCATC
TGCATGATTCAGAACACTTTCAACTTTAGTCTTAAACAGTCCAAGCACCT
TTTCCAAATTCTAATGGAGTGTATGGTTCACAGGGATTGTATTACCATAT
TTGATGCTGACTCTGAAGAGCAGCAAGACCTGGACTTAGCAATCCTAACA
GCTTTGCTGAAGGGCACAAATTTATCAGCGTCAGAGCAATTAAATCTGGC
AATGGCTTGGGACAGGGTGGACATTGCCAAGAAACATATCCTAATTTATG
AACAACACTGGAAGCCTGATGCCCTGGAACAAGCAATGTCAGATGCTTTA
GTGATGGATCGGGTGGATTTTGTGAAGCTCTTAATAGAATATGGAGTGAA
CCTCCATCGCTTTCTTACCATCCCTCGACTGGAAGAGCTCTACAATACAA
AACAAGGACCTACTAATACACTCTTGCATCATCTCGTCCAAGATGTGAAA
CAGCATACCCTTCTTTCAGGCTACCGAATAACCTTGATTGACATTGGATT
AGTAGTAGAATACCTCATTGGTAGAGCATATCGCAGCAACTACACTAGAA
AACATTTCAGAGCCCTCTACAACAACCTCTACAGAAAATACAAGCACCAG
AGACACTCCTCAGGAAATAGAAATGAGTCTGCAGAAAGTACGCTGCACTC
CCAGTTCATTAGAACTGCACAGCCATACAAATTCAAGGAAAAGTCTATAG
TCCTTCATAAATCAAGGAAGAAGTCAAAAGAACAAAATGTATCAGATGAC
CCTGAGTCTACTGGCTTTCTTTACCCTTACAATGACCTGCTGGTTTGGGC
TGTGCTGATGAAAAGGCAGAAGATGGCTATGTTCTTCTGGCAGCATGGAG
AGGAGGCCACGGTTAAAGCCGTGATTGCGTGTATCCTCTACCGGGCAATG
GCCCATGAAGCTAAGGAGAGTCACATGGTGGATGATGCCTCAGAAGAGTT
GAAGAATTACTCAAAACAGTTTGGCCAGCTGGCTCTGGACTTGTTGGAGA
AGGCATTCAAGCAGAATGAGCGCATGGCCATGACGCTGTTGACGTATGAA
CTCAGGAACTGGAGCAATTCGACCTGCCTTAAACTGGCCGTGTCGGGAGG
ATTACGACCCTTTGTTTCACATACTTGTACCCAGATGCTACTGACAGACA
TGTGGATGGGGAGGCTGAAAATGAGGAAAAACTCTTGGTTAAAGATTATT
ATAAGCATTATTTTACCACCCACCATTTTGACACTGGAATTTAAAAGCAA
AGCTGAGATGTCACATGTTCCCCAGTCCCAGGACTTCCAATTTATGTGGT
ATTACAGTGACCAGAACGCCAGCAGTTCCAAAGAAAGTGCTTCTGTGAAA
GAGTATGATTTGGAAAGGGGCCATGATGAGAAACTGGATGAAAATCAGCA
TTTTGGTTTGGAAAGTGGGCACCAACACCTTCCGTGGACCAGGAAAGTCT
ATGAGTTCTACAGTGCTCCAATTGTCAAGTTTTGGTTTTATACGATGGCG
TATTTGGCATTCCTCATGCTGTTCACTTACACCGTGTTGGTGGAGATGCA
GCCCCAGCCCAGCGTGCAGGAGTGGCTTGTTAGCATTTACATCTTCACCA
ATGCTATTGAGGTGGTCAGGGAGATCTGTATTTCAGAACCTGGGAAGTTT
ACCCAAAAGGTGAAGGTATGGATTAGTGAGTACTGGAACTTAACAGAAAC
TGTGGCCATTGGCCTGTTTTCAGCTGGCTTCGTCCTTCGATGGGGTGACC
CTCCTTTTCACACAGCGGGAAGACTGATCTACTGCATAGACATCATATTC
TGGTTCTCACGGCTCCTGGACTTCTTTGCTGTGAATCAACATGCAGGTCC
ATATGTGACCATGATTGCAAAAATGACAGCAAACATGTTCTATATTGTGA
TCATCATGGCCATAGTCCTGCTGAGCTTTGGAGTGGCACGCAAGGCCATC
CTTTCGCCAAAAGAGCCACCATCTTGGAGTCTAGCTCGAGATATTGTATT
TGAGCCATACTGGATGATATACGGAGAAGTCTATGCTGGAGAAATAGATG
TTTGTTCAAGCCAGCCATCCTGCCCTCCTGGTTCTTTTCTTACTCCATTC
TTGCAAGCTGTCTACCTCTTCGTGCAATATATCATCATGGTGAACCTGTT
GATTGCTTTCTTCAACAACGTTTACTTAGATATGGAATCCATTTCAAATA
ACCTGTGGAAATACAACCGCTATCGCTACATCATGACCTACCACGAGAAG
CCCTGGCTGCCCCCACCTCTCATCCTGCTGAGCCACGTGGGCCTTCTCCT
CCGCCGCCTGTGCTGTCATCGAGCTCCTCACGACCAAGAAGAGGGTGACG
TTGGATTAAAACTCTACCTCAGTAAGGAGGATCTGAAAAAACTTCATGAT
TTTGAGGAGCAGTGCGTGGAAAAATACTTCCATGAGAAGATGGAAGATGT
GAATTGTAGTTGTGAGGAACGAATCCGAGTGACATCAGAAAGGGTTACAG
AGATGTACTTCCAGCTGAAAGAAATGAATGAAAAGGTGTCTTTTATAAAG
GACTCCTTACTGTCTTTGGACAGCCAGGTGGGACACCTGCAGGATCTCTC
TGCCCTGACTGTGGATACCCTGAAAGTCCTTTCTGCTGTTGACACTTTGC
AAGAGGATGAGGCTCTCCTGGCCAAGAGAAAGCATTCTACTTGCAAAAAA
CTTCCCCACAGCTGGAGCAATGTCATCTGTGCAGAGGTTCTAGGCAGCAT
GGAGATCGCTGGAGAGAAGAAATACCAGTATTATAGCATGCCCTCTTCTT
TGCTGAGGAGCCTGGCTGGAGGCCGGCATCCCCCAAGAGTGCAGAGGGGG
GCACTTCTTGAGATTACAAACAGTAAAAGAGAGGCTACAAATGTAAGAAA
TGACCAGGAAAGGCAAGAAACACAAAGTAGTATAGTGGTTTCTGGGGTGT
CTCCTAACAGGCAAGCACACTCAAAGTATGGCCAGTTCCTTCTGGTCCCC
TCTAATCTAAAGCGAGTTCCTTTTTCAGCAGAAACTGTCTTGCCTCTGTC
CAGACCCTCTGTGCCAGATGTGCTGGCAACTGAACAGGACATCCAGACTG
AGGTTCTTGTTCATCTGACTGGGCAGACCCCAGTTGTCTCTGACTGGGCA
TCAGTGGATGAACCCAAGGAAAAGCACGAGCCTATTGCTCACTTACTGGA
TGGACAAGACAAGGCAGAGCAAGTGCTACCCACTTTGAGTTGCACACCTG
AACCCATGACAATGAGCTCCCCTCTTTCCCAAGCCAAGATCATGCAAACT
GGAGGTGGATATGTAAACTGGGCATTTTCAGAAGGTGATGAAACTGGTGT
GTTTAGCATCAAGAAAAAGTGGCAAACCTGCTTGCCCTCCACTTGTGACA
GTGATTCCTCTCGGAGTGAACAGCACCAGAAGCAGGCCCAGGACAGCTCC
CTATCTGATAACTCAACAAGATCGGCCCAGAGTAGTGAATGCTCAGAGGT
GGGACCATGGCTTCAGCCAAACACATCCTTTTGGATCAATCCTCTCCGCA
GATACAGGCCCTTCGCTAGGAGTCATAGTTTTAGATTCCATAAGGAGGAG
AAATTGATGAAGATCTGTAAGATTAAAAATCTTTCAGGCTCTTCAGAAAT
AGGGCAGGGAGCATGGGTCAAAGCGAAAATGCTAACCAAAGACAGGAGAC
TGTCAAAGAAAAAGAAGAATACTCAAGGACTCCAGGTGCCAATCATAACA
GTCAATGCCTGCTCTCAGAGTGACCAGTTGAATCCAGAGCCAGGAGAAAA
CAGCATCTCTGAAGAGGAGTACAGCAAGAACTGGTTCACAGTGTCCAAAT
TTAGTCACACAGGTGTAGAACCTTACATACATCAGAAAATGAAAACTAAA
GAAATTGGACAATGTGCTATACAAATCAGTGATTACCTAAAGCAGTCTCA
AGAGGATCTCAGCAAAAACTCTTTGTGGAATTCCAGGAGCACCAACCTCA
ATAGGAACTCCCTGCTGAAAAGTTCAATTGGAGTTGACAAGATCTCAGCC
TCCTTATAGAAAAGCCCTCAAGAGCCTCACCATCATTATTCAGCCATTGA
AAGGAATAATTTAATGAGGCTTTCTCAGACCATACCATTTACACCAGTCC
AACTGTTTGCAGGAGAAGAAATAACCGTCTACAGGTTGGAGGAGAGTTCC
CCTTTAAACCTTGATAAAAGCATGTCCTCTTGGTCTCAGCGTGGGAGAGC
GGCAATGATCCAGGTATTGTCCCGAGAGGAGATGGATGGGGGCCTCCGTA
AAGCTATGAGAGTCGTCAGCACTTGGTCTGAGGATGACATTCTCAAGCCG
GGACAAGTTTTCATTGTCAAGTCCTTTCTTCCTGAGGTTGTGCGGACATG
GCATAAAATCTTCCAGGAGAGCACTGTGCTTCATCTTTGCCTCAGGGAAA
TTCAACAACAAAGGGCTGCTCAAAAATTGATCTATACCTTCAACCAAGTG
AAACCACAAACCATACCCTACACACCAAGGTTCCTGGAAGTTTTCTTAAT
CTACTGCCATTCAGCCAACCAGTGGTTGACCATTGAGAAGTATATGACAG
GGGAGTTCCGGAAGTATAACAACAACAATGGTGATGAAATCACCCCCACC
AACACCCTGGAGGAGCTGATGTTGGCTTTCTCTCACTGGACCTATGAGTA
CACTCGGGGAGAGCTGCTGGTTTTAGATTTGCAAGGTGTTGGAGAAAATT
TGACAGATCCATCTGTTATAAAACCTGAAGTCAAACAATCAAGAGGAATG
GTGTTTGGACCGGCCAATTTGGGGGAAGATGCAATTAGAAACTTCATTGC
AAAACATCATTGTAACTCCTGCTGCCGGAAGCTCAAACTCCCGGATTTAA
AAAGAAATGACTATTCCCCTGAAAGGATAAATTCCACCTTTGGACTTGAG
ATAAAAATAGAATCAGCTGAGGAGCCTCCAGCAAGGGAGACGGGTAGAAA
TTCCCCAGAAGATGATATGCAACTATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRC
TPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTK
SPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLV
ISVHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGD
ALKSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLT
TLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVV
GLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGML
RPQVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQ
QDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDA
LEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTL
LHHLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYN
NLYRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKK
SKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAV
IACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNER
MAMTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKM
RKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNAS
SSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPI
VKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVRE
ICISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGR
LIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLL
SFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSC
PPGSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRY
RYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLS
KEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKE
MNEKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLA
KRKHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGG
RHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHS
KYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTG
QTPVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSP
LSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQ
HQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARS
HSFRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNT
QGLQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEP
YIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKS
SIGVDKISASL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44559
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 1-1700; ; All TRPM6 plasmids MUST be grown at 30 degrees C or less to prevent recombination.