cDNA Clone Senp8 (HCT116 cell line)

• Plasmid: pSC-B Senp8 ORF GFP SV40 & 3'UTR mut region
• Parent Plasmid: pSC-B
• Tag: GFP
• Species: Human

Sequence of Insert

ATGGACCCCGTAGTCTTGAGTTACATGGACAGTCTACTGCGGCAATCAGATGTCTCACTATTGGATCCGCCAAGCTGGCTCAATGACCATATTATTGGGTTTGCGTTTGAGTACTTTGCCAACAGTCAGTTTCATGACTGCTCTGATCACGTCAGTTTCATCAGCCCTGAAGTCACCCAGTTCATCAAGTGCACTAGCAACCCAGCAGAGATTGCCATGTTCCTTGAACCACTGGACCTCCCCAACAAGAGAGTTGTATTTTTAGCCATCAATGATAACTCCAACCAGGCAGCTGGAGGAACCCACTGGAGTTTATTGGTCTACCTCCAAGATAAAAATAGCTTTTTTCATTATGATTCCCATAGCAGGAGCAACTCAGTTCACGCAAAGCAGGTAGCAGAGAAACTGGAGGCTTTCTTAGGCAGAAAAGGAGACAAACTGGCCTTTGTGGAAGAGAAAGCCCCTGCCCAACAAAACAGCTAcGAtgcTGGGATGTACGTcATATGTAACACTGAGGCCTTGTGcCAaAACTTCTTTAGGCAACAGACAGAATCACTGCTGCAGCTACTCACCCCTGCATACATCACAAAGAAGAGGGGAGAATGGAAAGATCTCATTACCACACTTGCTAAAAAGTAG

Amino Acid Sequence

GSMDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNSNQAAGGTHWSLLVYLQDKNSFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDAGMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK*AAA

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU22909
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mut region from nt 481 - 530 TAcGAt gcT GGG ATG TAC GTcATA TGT AAC ACT GAG GCC TTG TGc CAa A ; Has BamH1 Not1 flanking sites for easier subcloning ; Has GFP SV40 tag inserted at nt510 using interanl NDE1 site (although GFP and SV40 are not in frame with mut region) needs to be mutated.