cDNA Clone 3x minidegron

• Plasmid: pRS413 ADH Promoter GFP 3x minidegron
• Parent Plasmid: pRS413
• Expressed: GFP - 3x minidegron
• Tag: GFP
• Species: Saccharomyces cerevisiae

Sequence of Insert

gtcgacatgAAAGAAAAGAGTGCTTGCCCAAAAGACCCTGCTAAACCTCCTGCCAAGGCTCAAGTTGTAGGATGGCCACCTGTTAGATCATACAGAAAGAATGTCATGGTTTCTTGTCAAAAGTCTTCAGGTGGACCAGAAGCTGCAGCCTTTGTTAAAGTATCTATGGATGGTGCCCCATATTTGAGAAAGATTGACTTAAGGATGTACAAGGGTGCTGGAGCAGGCGCCAAAGAAAAATCTGCCTGTCCAAAAGATCCTGCAAAGCCACCTGCTAAAGCACAAGTCGTGGGTTGGCCTCCTGTTAGAAGTTATAGAAAGAATGTGATGGTTTCTTGCCAAAAATCTAGTGGCGGTCCTGAAGCCGCAGCCTTCGTCAAAGTATCTATGGACGGCGCTCCTTATTTGCGTAAGATAGACTTGCGTATGTATAAAGGTGCCGGAGCTGGCGCAGGTGCTAAGGAAAAATCTGCGTGTCCAAAGGACCCTGCAAAACCACCAGCCAAGGCACAAGTTGTAGGTTGGCCCCCTGTAAGATCCTATAGAAAGAATGTTATGGTTTCTTGCCAAAAATCTTCTGGAGGCCCTGAAGCAGCTGCATTTGTTAAAGTTAGTATGGACGGTGCTCCTTACTTGAGAAAAATAGACTTGAGAATGTATAAATGAgggccc

Amino Acid Sequence

GSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLVD

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU22993
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: ADH Promoter cloned Sac Bam, GFP cloned Bam Sal. 3x minidegron cloned Sal Apa. All shown in Cassette field; Parental plasmid DU22964