cDNA Clone PLK4

• Synonyms: SAK; STK18; MCCRP2
• Plasmid: pGEX6P1 PLK4
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C PLK4
• Genbank: NM_014264.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGACCTGCATCGGGGAGAAGATCGAGGATTTTAAAGTTGG
AAATCTGCTTGGTAAAGGATCATTTGCTGGTGTCTACAGAGCTGAGTCCA
TTCACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGCCATG
TACAAAGCAGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTG
CCAATTGAAACATCCTTCTATCTTGGAGCTTTATAACTATTTTGAAGATA
GCAATTATGTGTATCTGGTATTAGAAATGTGCCATAATGGAGAAATGAAC
AGGTATCTAAAGAATAGAGTGAAACCCTTCTCAGAAAATGAAGCTCGACA
CTTCATGCACCAGATCATCACAGGGATGTTGTATCTTCATTCTCATGGTA
TACTACACCGGGACCTCACACTTTCTAACCTCCTACTGACTCGTAATATG
AACATCAAGATTGCTGATTTTGGGCTGGCAACTCAACTGAAAATGCCACA
TGAAAAGCACTATACATTATGTGGAACTCCTAACTACATTTCACCAGAAA
TTGCCACTCGAAGTGCACATGGCCTTGAATCTGATGTTTGGTCCCTGGGC
TGTATGTTTTATACATTACTTATCGGGAGACCACCCTTCGACACTGACAC
AGTCAAGAACACATTAAATAAAGTAGTATTGGCAGATTATGAAATGCCAT
CTTTTTTGTCAATAGAGGCCAAGGACCTTATTCACCAGTTACTTCGTAGA
AATCCAGCAGATCGTTTAAGTCTGTCTTCAGTATTGGACCATCCTTTTAT
GTCCCGAAATTCTTCAACAAAAAGTAAAGATTTAGGAACTGTGGAAGACT
CAATTGATAGTGGGCATGCCACAATTTCTACTGCAATTACAGCTTCTTCC
AGTACCAGTATAAGTGGTAGTTTATTTGACAAAAGAAGACTTTTGATTGG
TCAGCCACTCCCAAATAAAATGACTGTATTTCCAAAGAATAAAAGTTCAA
CTGATTTTTCTTCTTCAGGAGATGGAAACAGTTTTTATACTCAGTGGGGA
AATCAAGAAACCAGTAATAGTGGAAGGGGAAGAGTAATTCAAGATGCAGA
AGAAAGGCCACATTCTCGATACCTTCGTAGAGCTTATTCCTCTGATAGAT
CTGGCACTTCTAATAGTCAGTCTCAAGCAAAAACATATACAATGGAACGA
TGTCACTCAGCAGAAATGCTTTCAGTGTCCAAAAGATCAGGAGGAGGTGA
AAATGAAGAGAGGTACTCACCCACAGACAACAATGCCAACATTTTTAACT
TCTTTAAAGAAAAGACATCCAGTAGTTCTGGATCTTTTGAAAGACCTGAT
AACAATCAAGCACTCTCCAATCATCTTTGTCCAGGAAAAACTCCTTTTCC
ATTTGCAGACCCGACACCTCAGACTGAAACCGTACAACAGTGGTTTGGGA
ATCTGCAAATAAATGCTCATTTAAGAAAAACTACTGAATATGACAGCATC
AGCCCAAACCGGGACTTCCAGGGCCATCCAGATTTGCAGAAGGACACATC
AAAAAATGCCTGGACTGATACAAAAGTCAAAAAGAACTCTGATGCTTCTG
ATAATGCACATTCTGTAAAACAGCAAAATACCATGAAATATATGACTGCA
CTTCACAGTAAACCTGAGATAATCCAACAAGAATGTGTTTTTGGCTCAGA
TCCTCTTTCTGAACAGAGCAAGACTAGGGGTATGGAGCCACCATGGGGTT
ATCAGAATCGTACATTAAGAAGCATTACATCTCCGTTGGTTGCTCACAGG
TTAAAACCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATACTTGA
TTCAGAGGAGGTGTGTGTGGAGCTTGTAAAGGAGTATGCATCTCAAGAAT
ATGTGAAAGAAGTTCTTCAGATATCTAGTGATGGAAATACGATCACTATT
TATTATCCAAATGGTGGTAGAGGTTTTCCTCTTGCTGATAGACCACCCTC
ACCTACTGACAACATCAGTAGGTACAGCTTTGACAATTTACCAGAAAAAT
ACTGGCGAAAATATCAATATGCTTCCAGGTTTGTACAGCTTGTAAGATCT
AAATCTCCCAAAATCACTTATTTTACAAGATATGCTAAATGCATTTTGAT
GGAGAATTCTCCTGGTGCTGATTTTGAGGTTTGGTTTTATGATGGGGTAA
AAATACACAAAACAGAAGATTTCATTCAGGTGATTGAAAAGACAGGGAAG
TCTTACACTTTAAAAAGTGAAAGTGAAGTTAATAGCTTGAAAGAGGAGAT
AAAAATGTATATGGACCATGCTAATGAGGGTCATCGTATTTGTTTAGCAC
TGGAATCCATAATTTCAGAAGAGGAAAGGAAAACTAGGAGTGCTCCCTTT
TTCCCAATAATCATAGGAAGAAAACCTGGTAGTACTAGTTCACCTAAGGC
CTTATCACCTCCTCCTTCTGTGGATTCAAATTACCCAACGAGAGAGAGAG
CATCTTTCAACAGAATGGTCATGCATAGTGCTGCTTCTCCAACACAGGCA
CCAATCCTTAATCCCTCTATGGTTACAAATGAAGGACTTGGTCTTACAAC
TACAGCTTCTGGAACAGACATCTCTTCTAATAGTCTAAAAGATTGTCTTC
CTAAATCAGCACAACTTTTGAAATCTGTTTTTGTGAAAAATGTTGGTTGG
GCTACACAGTTAACTAGTGGAGCTGTGTGGGTTCAGTTTAATGATGGGTC
CCAGTTGGTTGTGCAGGCAGGAGTGTCTTCTATCAGTTATACCTCACCAA
ATGGTCAAACAACTAGGTATGGAGAAAATGAAAAATTACCAGACTACATC
AAACAGAAATTACAGTGTCTGTCTTCCATCCTTTTGATGTTTTCTAATCC
GACTCCTAATTTTCATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATCIGEKIEDFKVGNLLG
KGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKH
PSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQ
IITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHY
TLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNT
LNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNS
STKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLP
NKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPH
SRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEER
YSPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADP
TPQTETVQQWFGNLQINAHLRKTTEYDSISPNRDFQGHPDLQKDTSKNAW
TDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQECVFGSDPLSE
QSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEV
CVELVKEYASQEYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDN
ISRYSFDNLPEKYWRKYQYASRFVQLVRSKSPKITYFTRYAKCILMENSP
GADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYM
DHANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPP
PSVDSNYPTRERASFNRMVMHSAASPTQAPILNPSMVTNEGLGLTTTASG
TDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVV
QAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNF
H*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 PLK4171.68 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU43937
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No