cDNA Clone TTBK1

• Plasmid: pBABE Hygro FLAG-TTBK1 KD
• Parent Plasmid: pBabeD hygro FLAG
• Expressed: FLAG-TTBK1 KD
• Tag: FLAG

Sequence of Insert

ATGCAGTGCCTAGCGGCAGCTCTTAAGGACGAAACCAACATGAGTGGGGGAGGGGAGCAGGCCGACATCCTGCCGGCCAACTACGTGGTCAAAGATCGCTGGAAGGTGCTGAAAAAGATCGGGGGCGGGGGCTTCGGTGAGATCTATGAGGCCATGGACCTGCTGACCAGGGAGAACGTGGCTCTCAAGGTGGAGTCAGCCCAACAGCCTAAGCAGGTCCTCAAGATGGAGGTGGCTGTGCTCAAAAAGCTTCAAGGCAAGGACCACGTGTGCAGGTTCATTGGCTGTGGCCGTAACGAGAAGTTTAACTATGTGGTGATGCAGCTCCAGGGCCGGAACCTGGCTGACCTGCGCCGCAGCCAGCCAAGGGGCACTTTCACGTTGAGTACCACACTGCGCCTGGGCAAGCAGATCCTGGAGTCCATTGAAGCCATTCACTCCGTGGGCTTCCTGCACCGTGACATCAAGCCGTCCAACTTTGCCATGGGCCGGCTGCCCTCGACCTACAGGAAGTGCTACATGTTGGCCTTTGGGCTAGCCAGGCAGTACACTAACACCACTGGGGACGTCCGGCCTCCTCGGAATGTGGCCGGGTTCCGGGGGACTGTCCGCTACGCCTCGGTCAATGCTCACAAGAACCGGGAGATGGGCCGCCATGATGACCTGTGGTCCCTCTTCTACATGCTGGTGGAGTTTGCTGTGGGCCAGCTGCCCTGGAGGAAGATCAAAGACAAGGAGCAGGTGGGGATGATCAAAGAGAAATACGAGCACCGGATGCTACTGAAGCACATGCCATCCGAGTTTCATCTCTTCCTGGACCACATTGCCAGCCTCGACTACTTCACCAAGCCCGATTACCAGTTGATCATGTCAGTGTTTGAGAACAGCATGAAGGAGCGGGGCATTGCCGAGAACGAGGCCTTTGACTGGGAGAAGGCAGGCACTGATGCCCTCCTGTCCACGAGCACCTCCACCCCACCCCAGCAGAACACGCGACAGACCGCAGCCATGTTTGGAGTGGTCAATGTGACACCAGTGCCAGGGGATCTGCTGAGGGAAAATACCGAAGACGTGCTCCAGGGGGAACATCTGTCCGACCAGGAAAACGCTCCACCAATCCTGCCTGGAAGACCATCTGAGGGCCTCGGACCTAGTCCACACCTGGTGCCACATCCTGGAGGACCAGAAGCAGAAGTGTGGGAGGAAACCGACGTGAACCGAAATAAGCTGAGGATCAACATTGGCAAAAGCCCTTGCGTGGAGGAAGAGCAGTCCAGGGGAATGGGTGTGCCAAGCTCCCCTGTCAGAGCTCCCCCTGATAGCCCAACCACACCCGTGCGCTCCCTGCGATACAGGAGAGTCAATTCTCCTGAAAGTGAGCGACTCTCTACAGCAGACGGAAGGGTGGAACTGCCAGAGCGGCGCAGCCGAATGGATCTCCCTGGCAGTCCATCACGACAGGCCTGTTCTAGTCAGCCTGCTCAGATGCTGTCCGTGGACACTGGTCACGCCGATAGGCAGGCTTCTGGCAGAATGGACGTGAGCGCCTCCGTCGAACAGGAGGCACTGTCAAACGCCTTCAGAAGCGTGCCACTCGCCGAAGAGGAAGACTTTGATAGCAAGGAGTGGGTGATCATTGACAAGGAAACCGAGCTGAAAGATTTCCCACCAGGAGCAGAGCCATCTACAAGTGGAACTACCGATGAGGAACCTGAGGAACTGCGGCCACTCCCAGAGGAAGGAGAGGAACGAAGGAGACTGGGTGCAGAGCCTACAGTGCGCCCACGAGGCAGGAGCATGCAGGCACTCGCCGAGGAAGACCTGCAGCACCTCCCTCCACAGCCACTCCCACCTCAGCTGTCCCAGGGAGATGGACGCTCAGAGACCAGCCAGCCACCCACACCCGGTTCACCTAGCCACTCCCCACTGCATTCAGGACCTCGGCCACGGCGCCGAGAGTCCGACCCAACCGGACCACAGCGACAGGTGTTCTCCGTCGCTCCTCCATTTGAGGTCAATGGACTGCCAAGGGCAGTCCCTCTCAGCCTGCCATACCAGGATTTCAAGAGAGACCTGTCCGATTATAGAGAGCGAGCACGACTGCTCAACCGGGTGAGGAGAGTCGGATTTTCTCATATGCTGCTCACAACTCCTCAGGTGCCACTGGCACCCGTCCAGCCTCAGGCCAATGGGAAAGAGGAGGAAGAAGAGGAAGAGGAAGACGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGCCGCTGCAGCCGTGGCCCTCGGAGAGGTCCTGGGGCCTAGAAGCGGTTCAAGCTCCGAAGGCAGCGAGAGATCTACTGACAGGAGCCAGGAGGGAGCTCCAAGCACCCTGCTCGCAGACGATCAGAAGGAGTCCCGAGGCAGGGCCTCTATGGCTGACGGAGATCTGGAGCCTGAAGAGGGGTCTAAAACTCTCGTGCTGGTCAGTCCAGGCGACATGAAGAAATCACCCGTGACCGCAGAGCTCGCACCCGACCCTGATCTGGGAACTCTCGCTGCACTGACCCCACAGCACGAGAGGCCACAGCCAACAGGTAGTCAGCTGGACGTGAGCGAACCTGGCACTCTCTCTAGTGTCCTGAAGTCCGAGCCAAAACCACCTGGACCTGGTGCTGGACTGGGAGCAGGGACTGTGACCACAGGAGTCGGGGGTGTGGCTGTCACATCAAGCCCTTTCACTAAGGTGGAACGCACCTTTGTCCACATCGCCGAGAAAACCCATCTGAACGTGATGTCCTCTGGCGGACAGGCTCTCAGGAGCGAAGAGTTTTCCGCAGGAGGTGAACTCGGACTGGAGCTCGCTTCCGACGGAGGAGCAGTGGAAGAGGGAGCTAGAGCACCCCTGGAGAACGGGCTGGCCCTCTCTGGACTCAATGGGGCTGAAATTGAGGGCAGTGCCCTGTCAGGAGCTCCACGAGAAACACCTTCCGAGATGGCTACTAACTCTCTCCCAAATGGACCCGCCCTGGCTGATGGACCTGCCCCAGTGAGCCCACTGGAACCATCCCCTGAGAAGGTCGCAACCATCAGCCCTCGGCGCCATGCTATGCCAGGCTCTAGACCCCGGAGTCGCATTCCTGTGCTGCTCAGTGAAGAGGACACAGGGTCAGAACCATCTGGTAGTCTGTCAGCCAAGGAGCGGTGGTCTAAACGAGCAAGGCCTCAGCAGGATCTCGCCCGCCTGGTGATGGAGAAGAGGCAGGGAAGACTGCTCCTGAGGCTGGCTAGCGGGGCAAGTTCAAGCTCCTCTGAAGAGCAGCGAAGGGCTTCAGAGACACTGAGCGGTACTGGCTCCGAAGAGGACACACCTGCATCCGAGCCAGCCGCTGCACTGCCACGAAAATCTGGCCGAGCCGCTGCAACTAGAAGTAGAATCCCCCGGCCCATTGGACTGCGCATGCCTATGCCAGTGGCCGCTCAGCAGCCTGCTAGCAGATCCCACGGAGCAGCACCAGCACTGGACACTGCCATCACCTCTCGACTGCAGCTCCAGACCCCACCAGGCAGTGCCACAGCTGCAGATCTGAGGCCAAAGCAGCCTCCAGGACGAGGACTCGGTCCAGGAAGGGCACAGGCAGGAGCTAGACCCCCTGCACCTCGGTCCCCACGACTGCCAGCATCTACAAGTGCCGCTAGAAACGCATCAGCCAGCCCACGGTCCCAGTCTCTGAGTAGACGGGAGTCACCCAGCCCTTCCCATCAGGCACGACCAGGAGTGCCACCACCTCGAGGTGTCCCACCCGCTCGAGCACAGCCAGACGGAACCCCATCTCCAGGAGGTAGTAAGAAAGGTCCCAGGGGCAAGCTGCAGGCCCAGAGGGCAACTACTAAAGGTCGGGCAGGAGGTGCTGAAGGAAGGGCAGGGGCAAGGTGA

Amino Acid Sequence

MDYKDDDDKGSMQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLAFGLARQYTNTTGDVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIASLDYFTKPDYQLIMSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPILPGRPSEGLGPSPHLVPHPGGPEAEVWEETDVNRNKLRINIGKSPCVEEEQSRGMGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRVELPERRSRMDLPGSPSRQACSSQPAQMLSVDTGHADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFPPGAEPSTSGTTDEEPEELRPLPEEGEERRRLGAEPTVRPRGRSMQALAEEDLQHLPPQPLPPQLSQGDGRSETSQPPTPGSPSHSPLHSGPRPRRRESDPTGPQRQVFSVAPPFEVNGLPRAVPLSLPYQDFKRDLSDYRERARLLNRVRRVGFSHMLLTTPQVPLAPVQPQANGKEEEEEEEEDEEEEEEDEEEEEEEEEEEEEEEEEEEEEEEAAAAVALGEVLGPRSGSSSEGSERSTDRSQEGAPSTLLADDQKESRGRASMADGDLEPEEGSKTLVLVSPGDMKKSPVTAELAPDPDLGTLAALTPQHERPQPTGSQLDVSEPGTLSSVLKSEPKPPGPGAGLGAGTVTTGVGGVAVTSSPFTKVERTFVHIAEKTHLNVMSSGGQALRSEEFSAGGELGLELASDGGAVEEGARAPLENGLALSGLNGAEIEGSALSGAPRETPSEMATNSLPNGPALADGPAPVSPLEPSPEKVATISPRRHAMPGSRPRSRIPVLLSEEDTGSEPSGSLSAKERWSKRARPQQDLARLVMEKRQGRLLLRLASGASSSSSEEQRRASETLSGTGSEEDTPASEPAAALPRKSGRAAATRSRIPRPIGLRMPMPVAAQQPASRSHGAAPALDTAITSRLQLQTPPGSATAADLRPKQPPGRGLGPGRAQAGARPPAPRSPRLPASTSAARNASASPRSQSLSRRESPSPSHQARPGVPPPRGVPPARAQPDGTPSPGGSKKGPRGKLQAQRATTKGRAGGAEGRAGAR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42876
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Backbone: pBABE hygro FLAG ; Template: pcDNA5-FRT/TO-FLAG TTBK1 KD D176A (DU34542)