cDNA Clone TIR1 (oryza sativa)

• Plasmid: pRS413-Gal1,10 Pr -O.s.TIR1 delta aa1-260 -9Myc
• Parent Plasmid: pRS413 pGal1 10
• Expressed: TIR1 delta aa1-260-9Myc
• Genbank: Os04g0395600
• Tag: 9Myc
• Species: Oryza sativa

Sequence of Insert

gaattcATGTTGAGGAGTTTGTCTGGATTCTGGGATGCTTCTCCTGTTTG
CCTGTCATTTATCTACCCCCTGTGTGCTCAACTGACAGGATTGAACTTGA
GCTATGCACCCACACTTGATGCTTCTGACCTTACAAAAATGATTAGCCGC
TGTGTGAAGCTCCAACGCCTTTGGGTACTGGATTGTATCTCGGACAAAGG
CTTGCAAGTGGTGGCCTCCAGTTGCAAAGACTTGCAAGAACTCAGGGTAT
TTCCATCAGATTTCTACGTAGCTGGTTATTCTGCAGTGACAGAGGAGGGA
CTTGTTGCAGTATCCTTGGGCTGTCCAAAACTGAACTCACTACTGTACTT
CTGTCACCAAATGACTAATGCTGCACTAGTTACTGTCGCCAAGAACTGTC
CAAATTTCACACGATTCAGACTTTGTATTCTTGAGCCAGGGAAGCCTGAT
GTTGTGACAAGCCAACCATTAGATGAAGGCTTTGGAGCTATTGTTCGTGA
GTGCAAGGGATTACAACGTTTGTCAATATCTGGTCTTCTCACAGACAAAG
TTTTCATGTATATTGGGAAATATGCAAAACAACTTGAGATGCTTTCTATA
GCATTTGCTGGTGACAGTGATAAGGGTATGATGCATGTTATGAATGGATG
CAAGAATTTAAGGAAACTGGAGATAAGAGATAGCCCGTTTGGTGATGCTG
CACTCTTGGGGAATTTTGCTAGGTACGAGACAATGCGATCCCTTTGGATG
TCATCTTGCAATGTCACGTTAAAGGGGTGCCAAGTCCTTGCGTCAAAGAT
GCCGATGCTCAATGTTGAGGTCATAAATGAGCGGGATGGTAGCAATGAAA
TGGAGGAAAACCATGGAGATCTGCCTAAAGTGGAGAAATTATATGTGTAC
CGCACAACTGCTGGGGCGAGGGATGATGCACCAAATTTTGTTAAAATCCT
AGTCGACTCCGGTTCTGCTGCTAGTGGTGAACAAAAGTTGATTTCTGAAG
AAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGT
GAACAAAAATTAATCTCAGAAGAAGACTTGAACGGATCCTCTAGAGGTGA
ACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCT
CCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTG
AACGGATCCTCTAGAGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAA
CGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAAT
TAATCTCAGAAGAAGACTTGAACGGATCCACTAGCTAA

Amino Acid Sequence

MLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLTKMISRCV
KLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYSAVTEEGLV
AVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVV
TSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAF
AGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSS
CNVTLKGCQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRT
TAGARDDAPNFVKILVDSGSAASGEQKLISEEDLNGEQKLISEEDLNGEQ
KLISEEDLNGSSRGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNG
SSRGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSTS*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU24096
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Oryza sativa. cassette field shows Gal1,10 Pr through the ORF. 3' Apa1 site located 40bp approx downstream of ORF; ; parental DU25017