cDNA Clone TRPM6

• Synonyms: CHAK2, HMGX, HOMG, HOMG1, HSH
• Plasmid: pCMV5 HA TRPM6 L1721A K1804R IRES GFP
• Parent Plasmid: pCMV5D HA
• Expressed: HA-TRPM6 (L1721A + K1804R)-IRES-GFP
• Genbank: NM_017662.4
• Tag: HA
• Tag: GFP
• Species: Human

Sequence of Insert

ATGTACCCATACGACGTGCCAGACTACGCAAAAGAACAACCTGTCTTGGAGCGCTTGCAGTCCCAGAAATCCTGGATTAAAGGAGTATTTGACAAGAGAGAATGTAGCACAATCATACCCAGCTCAAAAAATCCTCACAGATGTACTCCAGTATGCCAAGTCTGCCAGAATTTAATCAGGTGTTACTGTGGCCGACTGATTGGAGACCATGCTGGGATAGATTATTCCTGGACCATCTCAGCTGCCAAGGGTAAAGAAAGTGAACAATGGTCTGTTGAAAAGCACACAACGAAAAGCCCAACAGATACTTTTGGCACGATTAATTTCCAAGATGGAGAGCACACCCATCATGCCAAGTATATTAGAACTTCTTATGATACAAAACTGGATCATCTGTTACATTTAATGTTGAAAGAGTGGAAAATGGAACTGCCCAAGCTTGTGATCTCAGTCCATGGGGGCATCCAGAACTTTACTATGCCCTCTAAATTTAAAGAGATTTTCAGCCAAGGTTTGGTTAAAGCTGCAGAGACAACCGGTGCGTGGATAATAACTGAAGGCATCAATACAGGAGTGTCCAAGCATGTTGGGGATGCCTTGAAATCCCATTCCTCTCATTCCTTGAGAAAAATCTGGACAGTTGGAATCCCTCCTTGGGGTGTCATTGAGAACCAGAGAGACCTTATTGGAAAAGATGTGGTGTGCCTGTACCAGACTCTGGATAACCCCCTCAGCAAGCTCACAACACTCAACAGCATGCACTCGCACTTCATCCTGTCTGATGATGGGACCGTGGGCAAGTATGGAAATGAAATGAAGCTCAGAAGGAACCTGGAGAAGTACCTCTCTCTGCAGAAAATACACTGCCGCTCAAGACAAGGCGTGCCGGTCGTGGGGCTGGTGGTGGAAGGCGGTCCCAACGTCATCCTGTCAGTGTGGGAGACTGTCAAGGACAAGGACCCAGTGGTGGTGTGTGAGGGTACAGGTAGGGCGGCTGACCTCCTGGCCTTCACACACAAACACCTGGCAGATGAAGGGATGCTGCGACCTCAGGTGAAAGAGGAGATCATCTGCATGATTCAGAACACTTTCAACTTTAGTCTTAAACAGTCCAAGCACCTTTTCCAAATTCTAATGGAGTGTATGGTTCACAGGGATTGTATTACCATATTTGATGCTGACTCTGAAGAGCAGCAAGACCTGGACTTAGCAATCCTAACAGCTTTGCTGAAGGGCACAAATTTATCAGCGTCAGAGCAATTAAATCTGGCAATGGCTTGGGACAGGGTGGACATTGCCAAGAAACATATCCTAATTTATGAACAACACTGGAAGCCTGATGCCCTGGAACAAGCAATGTCAGATGCTTTAGTGATGGATCGGGTGGATTTTGTGAAGCTCTTAATAGAATATGGAGTGAACCTCCATCGCTTTCTTACCATCCCTCGACTGGAAGAGCTCTACAATACAAAACAAGGACCTACTAATACACTCTTGCATCATCTCGTCCAAGATGTGAAACAGCATACCCTTCTTTCAGGCTACCGAATAACCTTGATTGACATTGGATTAGTAGTAGAATACCTCATTGGTAGAGCATATCGCAGCAACTACACTAGAAAACATTTCAGAGCCCTCTACAACAACCTCTACAGAAAATACAAGCACCAGAGACACTCCTCAGGAAATAGAAATGAGTCTGCAGAAAGTACGCTGCACTCCCAGTTCATTAGAACTGCACAGCCATACAAATTCAAGGAAAAGTCTATAGTCCTTCATAAATCAAGGAAGAAGTCAAAAGAACAAAATGTATCAGATGACCCTGAGTCTACTGGCTTTCTTTACCCTTACAATGACCTGCTGGTTTGGGCTGTGCTGATGAAAAGGCAGAAGATGGCTATGTTCTTCTGGCAGCATGGAGAGGAGGCCACGGTTAAAGCCGTGATTGCGTGTATCCTCTACCGGGCAATGGCCCATGAAGCTAAGGAGAGTCACATGGTGGATGATGCCTCAGAAGAGTTGAAGAATTACTCAAAACAGTTTGGCCAGCTGGCTCTGGACTTGTTGGAGAAGGCATTCAAGCAGAATGAGCGCATGGCCATGACGCTGTTGACGTATGAACTCAGGAACTGGAGCAATTCGACCTGCCTTAAACTGGCCGTGTCGGGAGGATTACGACCCTTTGTTTCACATACTTGTACCCAGATGCTACTGACAGACATGTGGATGGGGAGGCTGAAAATGAGGAAAAACTCTTGGTTAAAGATTATTATAAGCATTATTTTACCACCCACCATTTTGACACTGGAATTTAAAAGCAAAGCTGAGATGTCACATGTTCCCCAGTCCCAGGACTTCCAATTTATGTGGTATTACAGTGACCAGAACGCCAGCAGTTCCAAAGAAAGTGCTTCTGTGAAAGAGTATGATTTGGAAAGGGGCCATGATGAGAAACTGGATGAAAATCAGCATTTTGGTTTGGAAAGTGGGCACCAACACCTTCCGTGGACCAGGAAAGTCTATGAGTTCTACAGTGCTCCAATTGTCAAGTTTTGGTTTTATACGATGGCGTATTTGGCATTCCTCATGCTGTTCACTTACACCGTGTTGGTGGAGATGCAGCCCCAGCCCAGCGTGCAGGAGTGGCTTGTTAGCATTTACATCTTCACCAATGCTATTGAGGTGGTCAGGGAGATCTGTATTTCAGAACCTGGGAAGTTTACCCAAAAGGTGAAGGTATGGATTAGTGAGTACTGGAACTTAACAGAAACTGTGGCCATTGGCCTGTTTTCAGCTGGCTTCGTCCTTCGATGGGGTGACCCTCCTTTTCACACAGCGGGAAGACTGATCTACTGCATAGACATCATATTCTGGTTCTCACGGCTCCTGGACTTCTTTGCTGTGAATCAACATGCAGGTCCATATGTGACCATGATTGCAAAAATGACAGCAAACATGTTCTATATTGTGATCATCATGGCCATAGTCCTGCTGAGCTTTGGAGTGGCACGCAAGGCCATCCTTTCGCCAAAAGAGCCACCATCTTGGAGTCTAGCTCGAGATATTGTATTTGAGCCATACTGGATGATATACGGAGAAGTCTATGCTGGAGAAATAGATGTTTGTTCAAGCCAGCCATCCTGCCCTCCTGGTTCTTTTCTTACTCCATTCTTGCAAGCTGTCTACCTCTTCGTGCAATATATCATCATGGTGAACCTGTTGATTGCTTTCTTCAACAACGTTTACTTAGATATGGAATCCATTTCAAATAACCTGTGGAAATACAACCGCTATCGCTACATCATGACCTACCACGAGAAGCCCTGGCTGCCCCCACCTCTCATCCTGCTGAGCCACGTGGGCCTTCTCCTCCGCCGCCTGTGCTGTCATCGAGCTCCTCACGACCAAGAAGAGGGTGACGTTGGATTAAAACTCTACCTCAGTAAGGAGGATCTGAAAAAACTTCATGATTTTGAGGAGCAGTGCGTGGAAAAATACTTCCATGAGAAGATGGAAGATGTGAATTGTAGTTGTGAGGAACGAATCCGAGTGACATCAGAAAGGGTTACAGAGATGTACTTCCAGCTGAAAGAAATGAATGAAAAGGTGTCTTTTATAAAGGACTCCTTACTGTCTTTGGACAGCCAGGTGGGACACCTGCAGGATCTCTCTGCCCTGACTGTGGATACCCTGAAAGTCCTTTCTGCTGTTGACACTTTGCAAGAGGATGAGGCTCTCCTGGCCAAGAGAAAGCATTCTACTTGCAAAAAACTTCCCCACAGCTGGAGCAATGTCATCTGTGCAGAGGTTCTAGGCAGCATGGAGATCGCTGGAGAGAAGAAATACCAGTATTATAGCATGCCCTCTTCTTTGCTGAGGAGCCTGGCTGGAGGCCGGCATCCCCCAAGAGTGCAGAGGGGGGCACTTCTTGAGATTACAAACAGTAAAAGAGAGGCTACAAATGTAAGAAATGACCAGGAAAGGCAAGAAACACAAAGTAGTATAGTGGTTTCTGGGGTGTCTCCTAACAGGCAAGCACACTCAAAGTATGGCCAGTTCCTTCTGGTCCCCTCTAATCTAAAGCGAGTTCCTTTTTCAGCAGAAACTGTCTTGCCTCTGTCCAGACCCTCTGTGCCAGATGTGCTGGCAACTGAACAGGACATCCAGACTGAGGTTCTTGTTCATCTGACTGGGCAGACCCCAGTTGTCTCTGACTGGGCATCAGTGGATGAACCCAAGGAAAAGCACGAGCCTATTGCTCACTTACTGGATGGACAAGACAAGGCAGAGCAAGTGCTACCCACTTTGAGTTGCACACCTGAACCCATGACAATGAGCTCCCCTCTTTCCCAAGCCAAGATCATGCAAACTGGAGGTGGATATGTAAACTGGGCATTTTCAGAAGGTGATGAAACTGGTGTGTTTAGCATCAAGAAAAAGTGGCAAACCTGCTTGCCCTCCACTTGTGACAGTGATTCCTCTCGGAGTGAACAGCACCAGAAGCAGGCCCAGGACAGCTCCCTATCTGATAACTCAACAAGATCGGCCCAGAGTAGTGAATGCTCAGAGGTGGGACCATGGCTTCAGCCAAACACATCCTTTTGGATCAATCCTCTCCGCAGATACAGGCCCTTCGCTAGGAGTCATAGTTTTAGATTCCATAAGGAGGAGAAATTGATGAAGATCTGTAAGATTAAAAATCTTTCAGGCTCTTCAGAAATAGGGCAGGGAGCATGGGTCAAAGCGAAAATGCTAACCAAAGACAGGAGACTGTCAAAGAAAAAGAAGAATACTCAAGGACTCCAGGTGCCAATCATAACAGTCAATGCCTGCTCTCAGAGTGACCAGTTGAATCCAGAGCCAGGAGAAAACAGCATCTCTGAAGAGGAGTACAGCAAGAACTGGTTCACAGTGTCCAAATTTAGTCACACAGGTGTAGAACCTTACATACATCAGAAAATGAAAACTAAAGAAATTGGACAATGTGCTATACAAATCAGTGATTACCTAAAGCAGTCTCAAGAGGATCTCAGCAAAAACTCTTTGTGGAATTCCAGGAGCACCAACCTCAATAGGAACTCCCTGCTGAAAAGTTCAATTGGAGTTGACAAGATCTCAGCCTCCTTAAAAAGCCCTCAAGAGCCTCACCATCATTATTCAGCCATTGAAAGGAATAATTTAATGAGGGCTTCTCAGACCATACCATTTACACCAGTCCAACTGTTTGCAGGAGAAGAAATAACCGTCTACAGGTTGGAGGAGAGTTCCCCTTTAAACCTTGATAAAAGCATGTCCTCTTGGTCTCAGCGTGGGAGAGCGGCAATGATCCAGGTATTGTCCCGAGAGGAGATGGATGGGGGCCTCCGTAAAGCTATGAGAGTCGTCAGCACTTGGTCTGAGGATGACATTCTCAAGCCGGGACAAGTTTTCATTGTCAGGTCCTTTCTTCCTGAGGTTGTGCGGACATGGCATAAAATCTTCCAGGAGAGCACTGTGCTTCATCTTTGCCTCAGGGAAATTCAACAACAAAGGGCTGCTCAAAAATTGATCTATACCTTCAACCAAGTGAAACCACAAACCATACCCTACACACCAAGGTTCCTGGAAGTTTTCTTAATCTACTGCCATTCAGCCAACCAGTGGTTGACCATTGAGAAGTATATGACAGGGGAGTTCCGGAAGTATAACAACAACAATGGTGATGAAATCACCCCCACCAACACCCTGGAGGAGCTGATGTTGGCTTTCTCTCACTGGACCTATGAGTACACTCGGGGAGAGCTGCTGGTTTTAGATTTGCAAGGTGTTGGAGAAAATTTGACAGATCCATCTGTTATAAAACCTGAAGTCAAACAATCAAGAGGAATGGTGTTTGGACCGGCCAATTTGGGGGAAGATGCAATTAGAAACTTCATTGCAAAACATCATTGTAACTCCTGCTGCCGGAAGCTCAAACTCCCGGATTTAAAAAGAAATGACTATTCCCCTGAAAGGATAAATTCCACCTTTGGACTTGAGATAAAAATAGAATCAGCTGAGGAGCCTCCAGCAAGGGAGACGGGTAGAAATTCCCCAGAAGATGATATGCAACTATAA

Amino Acid Sequence

MYPYDVPDYAKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTP
VCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSP
TDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVIS
VHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDAL
KSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTL
NSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGL
VVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRP
QVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQD
LDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALE
QAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLH
HLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNL
YRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSK
EQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIA
CILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMA
MTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRK
NSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSS
KESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVK
FWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREIC
ISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLI
YCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSF
GVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPP
GSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRY
IMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKE
DLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMN
EKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKR
KHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRH
PPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKY
GQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQT
PVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLS
QAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQ
KQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHS
FRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQG
LQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYI
HQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSI
GVDKISASLKSPQEPHHHYSAIERNNLMRASQTIPFTPVQLFAGEEITVY
RLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSE
DDILKPGQVFIVRSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLI
YTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNG
DEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEV
KQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERIN
STFGLEIKIESAEEPPARETGRNSPEDDMQL*.......IRES........MATTMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44770
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: L1721A + K1804R (KD); ; All TRPM6 plasmids MUST be grown at 30 degrees C or less to prevent recombination.