cDNA Clone Uba4

• Synonyms: YHR1
• Plasmid: pRS413-ADH Pr-3HA-Uba4 C225S
• Parent Plasmid: pRS413 pADH 3HA
• Expressed: 3HA-Uba4 C225S
• Genbank: NM_001179241.1
• Tag: 3HA
• Species: Saccharomyces cerevisiae

Sequence of Insert

gaattcATGAATGACTACCATCTCGAGGATACCACGTCTGAACTTGAAGCATTAAG
ATTGGAGAATGCACAATTAAGAGAGCAGCTTGCCAAGAGAGAAGACAGCA
GCCGCGACTACCCATTATCTTTGGAAGAATACCAACGTTACGGAAGACAA
ATGATTGTTGAAGAAACAGGTGGTGTAGCAGGTCAAGTCAAGTTGAAAAA
TACAAAAGTTTTGGTAGTTGGTGCTGGAGGTTTGGGATGTCCCGCCTTGC
CCTACTTAGCGGGCGCTGGCGTGGGCCAAATAGGCATAGTAGATAACGAT
GTGGTGGAGACTTCCAATTTGCATAGGCAGGTTCTTCATGATTCTAGCAG
AGTTGGAATGTTGAAATGTGAGTCGGCCAGGCAATATATCACGAAACTGA
ACCCACACATTAACGTCGTTACCTATCCCGTTAGATTGAACTCCAGTAAT
GCTTTTGACATTTTCAAAGGTTACAATTATATATTAGACTGCACAGATTC
TCCATTAACGAGATACCTGGTGTCTGATGTTGCAGTTAATCTGGGAATAA
CAGTAGTGTCTGCATCCGGTTTGGGAACAGAGGGCCAGCTAACTATATTG
AATTTTAACAATATAGGGCCATGCTACAGATGCTTTTATCCAACACCTCC
GCCACCAAATGCCGTGACCTCTTcCCAAGAAGGCGGTGTGATAGGACCTT
GCATTGGACTAGTTGGAACAATGATGGCTGTAGAAACTTTGAAGCTTATC
CTAGGAATCTACACCAATGAAAATTTTAGTCCCTTTTTGATGTTATATTC
AGGTTTCCCACAGCAGAGCCTGCGCACTTTCAAAATGAGAGGCAGACAAG
AAAAGTGTCTATGCTGTGGTAAGAATCGAACGATAACAAAAGAAGCCATC
GAAAAAGGTGAGATCAATTACGAACTGTTTTGTGGCGCACGAAACTATAA
TGTATGCGAGCCTGATGAGAGAATCAGTGTGGACGCATTTCAGCGTATCT
ACAAGGATGATGAATTTCTAGCAAAACATATATTTCTTGATGTTAGGCCA
TCCCATCATTACGAGATATCTCATTTCCCTGAAGCAGTTAATATTCCAAT
CAAAAATCTAAGAGATATGAACGGTGATCTTAAGAAACTGCAAGAAAAAC
TTCCCAGTGTAGAAAAAGACAGTAATATAGTGATTCTTTGCCGCTACGGT
AACGACTCTCAGCTAGCTACAAGATTATTGAAGGATAAATTTGGGTTTTC
TAATGTACGAGACGTGAGAGGAGGATACTTCAAATACATAGACGATATTG
ATCAAACCATTCCTAAATATTAGgtcgac

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFMNDYHLEDTTSELEALRL
ENAQLREQLAKREDSSRDYPLSLEEYQRYGRQMIVEETGGVAGQVKLKNT
KVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRV
GMLKCESARQYITKLNPHINVVTYPVRLNSSNAFDIFKGYNYILDCTDSP
LTRYLVSDVAVNLGITVVSASGLGTEGQLTILNFNNIGPCYRCFYPTPPP
PNAVTSSQEGGVIGPCIGLVGTMMAVETLKLILGIYTNENFSPFLMLYSG
FPQQSLRTFKMRGRQEKCLCCGKNRTITKEAIEKGEINYELFCGARNYNV
CEPDERISVDAFQRIYKDDEFLAKHIFLDVRPSHHYEISHFPEAVNIPIK
NLRDMNGDLKKLQEKLPSVEKDSNIVILCRYGNDSQLATRLLKDKFGFSN
VRDVRGGYFKYIDDIDQTIPKY*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU24122
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: chromosome VIII coordinates 333072 to 334394