cDNA Clone SYT1

• Synonyms: P65, SVP65, SYT
• Plasmid: pcDNA5frtTO HA mSTAR SUMO SYT1 272-end
• Parent Plasmid: pFastbacD 6His Sumo Star
• Expressed: HA-mSTAR SUMO-SYT1 272-end
• Genbank: NM_001135805.1
• Tag: HA
• Tag: mSTAR
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCCATGGAGAAATTGGGTGATATCTGCTTCTCCCTTCGCTACGTACCTACTGCTGGTAAGCTGACTGTTGTCATTCTGGAGGCAAAGAACCTGAAGAAGATGGATGTGGGTGGCTTATCCGATCCTTATGTGAAGATTCATCTGATGCAGAATGGTAAGAGGCTGAAGAAGAAAAAGACAACAATTAAAAAGAACACACTTAACCCCTACTACAATGAGTCATTCAGCTTTGAAGTACCTTTTGAACAAATCCAGAAAGTGCAGGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGATGCCATCGGCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCAGGCGACCTATTGCCCAGTGGCACACCCTGCAGGTAGAGGAGGAAGTTGATGCCATGCTGGCCGTCAAGAAGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSATMSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHF
KVKMTTHLKKLKESYCQRQGVPMNSLTFLFEGQEIADNHAPKELGMEEED
VIEVYQEQTGGAGSMEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVG
GLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKV
QVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWH
TLQVEEEVDAMLAVKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44824
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 272-end. Contains a modified SUMO which should be resistant to cleavage in mammalian cells.