cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pcDNA5frtTO FLAG TTBK2
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG-TTBK2
• Genbank: NM_173500.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCATGAGTGGGGGAGGAGAGCAGCCGGATATCCTGAGTGTTGGAATCCTAGTGAAAGAAAGATGGAAAGTGTTGAGAAAGATTGGGGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAAAATGTTGCACTGAAGGTGGAATCAGCTCAACAACCAAAACAAGTTCTGAAAATGGAAGTTGCTGTTTTGAAAAAGCTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGGAATGATCGATTCAACTATGTGGTCATGCAGTTGCAGGGTCGGAATCTGGCAGATCTTCGCCGTAGCCAGTCCCGAGGCACATTCACCATTAGTACCACTCTCCGGCTGGGTAGACAGATTTTGGAGTCTATTGAAAGCATTCATTCTGTGGGATTCTTGCATCGAGACATCAAACCGTCGAACTTCGCTATGGGTCGCTTTCCTAGTACATGTAGGAAATGTTACATGCTTGATTTTGGCTTGGCTCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTCGAGCTGTGGCAGGTTTTCGAGGGACAGTTCGTTATGCATCAATCAACGCACATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCCTTATTCTACATGTTGGTGGAGTTTGTGGTTGGTCAGCTGCCCTGGAGAAAAATAAAGGACAAGGAGCAAGTAGGCTCTATTAAGGAGAGATATGACCACAGGCTCATGTTGAAACATCTCCCTCCAGAATTCAGCATCTTTCTAGACCATATCTCTTCTTTGGATTATTTTACAAAACCAGACTACCAGCTTCTTACATCCGTGTTTGACAATAGCATCAAGACTTTTGGAGTAATTGAGAGTGACCCTTTTGACTGGGAGAAGACTGGAAATGATGGCTCCCTAACAACCACCACTACTTCTACCACCCCTCAGTTGCACACTCGCTTGACCCCTGCTGCAATTGGAATTGCCAATGCTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATACAGATGAGGTATTTCCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCCCTGTTGGTGTGTCACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGTCCCCAGGAGAAGGATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGATAAAGCTTGGAATTTGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCCAGGCAAATGGTCTTCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTCCGCTCAGAGATTACTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTTACGTTCCATTCACAGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAAAGCCAGACACTGACAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAAGATACCAGTGCAGGAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCCTTGCGTTCCTGCTGTGTCCCGTACTGACCACATCTGGCACTATGATGAAGAATATCTTCCAGATGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAGGCAGATGGTGGTGGCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTGCAAGCAAGAAATTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGGACCTTCAGGATTTTAGGACAAATGAGGCTGTAGGACATAAAACAACTGGAAGTCCTTCTGATGAGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACCTCAAGATGAAAAGCTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTCAGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAGAACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTTATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCTTACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTTCAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCCATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTTGGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGGGGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAAAGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAGACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAAGCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAGCATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAAGGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTTTTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTGACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGAAGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGGATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCTACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGGAGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGAAGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTTTTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGCAAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGCTTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGTGCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTTTTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTCACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGTCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCAGGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAAAGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTCCTAGGGAGTAGTAACTCCGACTCAGACCTTTTCTCCCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAACCCCGGAGCACTACTCAGTGCAAGAGTCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTCCCTCGCAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGCACGTCTAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTTCGTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCTTCCTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATGGCCTGAAAGGCTCCGGCAGCCTCCACCACCACTCAGCCAGCACTAAAACCCCCCAAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGATAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSAMSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDA
LDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDR
FNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFL
HRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRG
TVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSI
KERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFG
VIESDPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDL
LRENTDEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDA
NKNKIKLGICKAATEEENSHGQANGLLNAPSLGSPIRVRSEITQPDRDIP
LVRKLRSIHSFELEKRLTLEPKPDTDKFLETCLEKMQKDTSAGKESILPA
LLHKPCVPAVSRTDHIWHYDEEYLPDASKPASANTPEQADGGGSNGFIAV
NLSSCKQEIDSKEWVIVDKEQDLQDFRTNEAVGHKTTGSPSDEEPEVLQV
LEASPQDEKLQLGPWAENDHLKKETSGVVLALSAEGPPTAASEQYTDRLE
LQPGAASQFIAATPTSLMEAQAEGPLTAITIPRPSVASTQSTSGSFHCGQ
QPEKKDLQPMEPTVELYSPRENFSGLVVTEGEPPSGGSRTDLGLQIDHIG
HDMLPNIRESNKSQDLGPKELPDHNRLVVREFENLPGETEEKSILLESDN
EDEKLSRGQHCIEISSLPGDLVIVEKDHSATTEPLDVTKTQTFSVVPNQD
KNNEIMKLLTVGTSEISSRDIDPHVEGQIGQVAEMQKNKISKDDDIMSED
LPGHQGDLSTFLHQEGKREKITPRNGELFHCVSENEHGAPTRKDMVRSSF
VTRHSRIPVLAQEIDSTLESSSPVSAKEKLLQKKAYQPDLVKLLVEKRQF
KSFLGDLSSASDKLLEEKLATVPAPFCEEEVLTPFSRLTVDSHLSRSAED
SFLSPIISQSRKSKIPRPVSWVNTDQVNSSTSSQFFPRPPPGKPPTRPGV
EARLRRYKVLGSSNSDSDLFSRLAQILQNGSQKPRSTTQCKSPGSPHNPK
TPPKSPVVPRRSPSASPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRS
KSPPSHSGSSSSRRSCQQEHCKPSKNGLKGSGSLHHHSASTKTPQGKSKP
ASKLSR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44862
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Full length, internal BamHI knocked out thus this clone may be used as a source of full-length fragment for shuttling.