cDNA Clone SIK3 (Long)

• Plasmid: pFB SIK3 GST
• Parent Plasmid: pFastbac C-GST
• Expressed: GST-SIK3-His6
• Tag: GST
• Species: Human

Sequence of Insert

ggatCCGAAACCATGGccGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGC
TGCCGGGGCCGGGACTGGGGGAGCCGGGCCCGCGGGCCGCCTGCTGCCTC
CGCCCGCGCCGGGGTCCCCAGCCGCCCCCGCTGCCGTGTCCCCTGCGGCC
GGCCAGCCGCGTCCCCCAGCCCCGGCCTCCCGCGGACCCATGCCCGCCCG
TATCGGCTACTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCGG
TGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTATCAAG
ATCATAGATAAGACCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCG
GGAAGTTCAAATTATGAAGATGCTTTGCCACCCCCATATCATCAGGCTCT
ACCAGGTTATGGAGACAGAACGGATGATTTATCTGGTGACAGAATATGCT
AGTGGAGGGGAAATATTTGACCACCTGGTGGCCCATGGTAGAATGGCAGA
AAAGGAGGCACGTCGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTTT
GTCACTGTCGGAACATTGTTCATCGTGATTTAAAAGCTGAAAATTTACTT
CTGGATGCCAATCTGAATATCAAAATAGCAGATTTTGGTTTCAGTAACCT
CTTCACTCCTGGGCAGCTGCTGAAGACCTGGTGTGGCAGCCCTCCCTATG
CTGCACCTGAACTCTTTGAAGGAAAAGAATATGATGGGCCCAAAGTGGAC
ATCTGGAGCCTTGGAGTTGTCCTCTACGTGCTTGTGTGCGGTGCCCTGCC
ATTTGATGGAAGCACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGAA
AGTTCCGCATCCCATTTTTTATGTCCACAGAATGTGAGCATTTGATCCGC
CATATGTTGGTGTTAGATCCCAATAAGCGCCTCTCCATGGAGCAGATCTG
CAAGCACAAGTGGATGAAGCTAGGGGACGCCGATCCCAACTTTGACAGGT
TAATAGCTGAATGCCAACAACTAAAGGAAGAAAGACAGGTGGACCCCCTG
AATGAGGATGTCCTCTTGGCCATGGAGGACATGGGACTGGACAAAGAACA
GACACTGCAGTCATTAAGATCAGATGCCTATGATCACTATAGTGCAATCT
ACAGCCTGCTGTGTGATCGACATAAGAGACATAAAACCCTGCGTCTCGGA
GCACTTCCTAGCATGCCCCGAGCCCTGGCCTTTCAAGCACCAGTCAATAT
CCAGGCGGAGCAGGCAGGTACTGCTATGAACATCAGCGTTCCCCAGGTGC
AGCTGATCAACCCAGAGAACCAAATTGTGGAGCCGGATGGGACACTGAAT
TTGGACAGTGATGAGGGTGAAGAGCCTTCCCCTGAAGCATTGGTGCGCTA
TTTGTCAATGAGGAGGCACACAGTGGGTGTGGCTGACCCACGCACGGAAG
TTATGGAAGATCTGCAGAAGCTCCTACCTGGCTTTCCTGGAGTCAACCCC
CAGGCTCCATTCCTGCAGGTGGCCCCTAATGTGAACTTCATGCACAACCT
GTTGCCTATGCAAAACTTGCAACCAACCGGGCAACTTGAGTACAAGGAGC
AGTCTCTCCTACAGCCGCCCACGCTACAGCTGTTGAATGGAATGGGCCCC
CTTGGCCGGAGGGCATCAGATGGAGGAGCCAACATCCAACTGCATGCCCA
GCAGCTGCTGAAGCGCCCACGGGGACCCTCTCCGCTTGTCACCATGACAC
CAGCAGTGCCAGCAGTTACCCCTGTGGACGAGGAGAGCTCAGACGGGGAG
CCAGACCAGGAAGCTGTGCAGAGGTACTTGGCAAATAGGTCCAAAAGACA
TACACTGGCCATGACCAACCCTACAGCTGAGATCCCACCGGACCTACAAC
GGCAGCTAGGACAGCAGCCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTG
GTACCTGATCAGCATCGCTCTACCTACAAGGACTCCAACACTCTGCACCT
CCCTACGGAGCGTTTCTCCCCTGTGCGCCGGTTCTCAGATGGGGCTGCGA
GCATCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAACAACAGCAGC
ATCAAACAGCTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGG
GCAGATTGATGAAAGAACCCTGGAGAAGACCCAGCAGCAGCATATGTTAT
ACCAGCAGGAGCAGCACCATCAAATTCTCCAGCAACAAATTCAAGACTCT
ATCTGTCCTCCTCAGCCATCTCCACCTCTTCAGGCTGCATGTGAAAATCA
GCCAGCCCTCCTTACCCATCAGCTCCAGAGGTTAAGGATTCAGCCTTCAA
GCCCACCCCCCAACCACCCCAACAACCATCTCTTCAGGCAGCCCAGTAAT
AGTCCTCCCCCCATGAGCAGTGCCATGATCCAGCCTCACGGGGCTGCATC
TTCTTCCCAGTTTCAAGGCTTACCTTCCCGCAGTGCAATCTTTCAGCAGC
AACCTGAGAACTGTTCCTCTCCTCCCAACGTGGCACTAACCTGCTTGGGT
ATGCAGCAGCCTGCTCAGTCACAGCAGGTCACCATCCAAGTCCAAGAGCC
TGTTGACATGCTCAGCAACATGCCAGGCACAGCTGCAGGCTCCAGTGGGC
GCGGCATCTCCATCAGCCCCAGTGCTGGTCAGATGCAGATGCAGCACCGT
ACCAACCTGATGGCCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGCA
GCTGAGTGCTGACAGTGCAGAGGCTCACAGCTTGAACGTGAATCGGTTCT
CCCCTGCTAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGAC
CAGTCCCGGGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTT
CTCTCCAACCCAAGCCCTGAAAGTCCCTCCACTTGACCAATTCCCCACCT
TCCCTCCCAGTGCACATCAGCAGCCGCCACACTATACCACGTCGGCACTA
CAGCAGGCCCTGCTGTCTCCCACGCCGCCAGACTATACAAGACACCAGCA
GGTACCCCACATCCTTCAAGGACTGCTTTCTCCCCGGCATTCGCTCACCG
GCCACTCGGACATCCGGCTGCCCCCAACAGAGTTTGCACAGCTCATTAAA
AGGCAGCAGCAACAACGGCAGCAGCAGCAGCAACAGCAGCAACAGCAAGA
ATACCAGGAACTGTTCAGGCACATGAACCAAGGGGATGCGGGGAGTCTGG
CTCCCAGCCTTGGGGGACAGAGCATGACAGAGCGCCAGGCTTTATCTTAT
CAAAATGCTGACTCTTATCACCATCACACCAGCCCCCAGCATCTGCTACA
AATCAGGGCACAAGAATGTGTCTCACAGGCTTCCTCACCCACCCCGCCCC
ACGGGTATGCTCACCAGCCGGCACTGATGCATTCAGAGAGCATGGAGGAG
GACTGCTCGTGTGAGGGGGCCAAGGATGGCTTCCAAGACAGTAAGAGTTC
AAGTACATTGACCAAAGGTTGCCATGACAGCCCTCTGCTCTTGAGTACCG
GTGGACCTGGGGACCCTGAATCTTTGCTAGGAACTGTGAGTCATGCCCAA
GAATTGGGGATACATCCCTATGGTCATCAGCCAACTGCTGCATTCAGTAA
AAATAAGGTGCCCAGCAGAGAGCCTGTCATAGGGAACTGCATGGATAGAA
GTTCTCCAGGACAAGCAGTGGAGCTGCCGGATCACAATGGGCTCGGGTAC
CCAGCACGCCCCTCCGTCCATGAGCACCACAGGCCCCGGGCCCTCCAGAG
ACACCACACGATCCAGAACAGCGACGATGCTTATGTACAGCTGGATAACT
TGCCAGGAATGAGTCTCGTGGCTGGGAAAGCACTTAGCTCTGCCCGGATG
TCGGATGCAGTTCTCAGTCAGTCTTCGCTCATGGGCAGCCAGCAGTTTCA
GGATGGGGAAAATGAGGAATGTGGGGCAAGCCTGGGAGGTCATGAGCACC
CAGACCTGAGTGATGGCAGCCAGCATTTAAACTCCTCTTGCTATCCATCT
ACGTGTATTACAGACATTCTGCTCAGCTACAAGCACCCCGAAGTCTCCTT
CAGCATGGAGCAGGCAGGCGTGgcggccgc

Amino Acid Sequence

MAAAAAASGAGGAAGAGTGGAGPAGRLLPPPAPGSPAAPAAVSPAAGQPR
PPAPASRGPMPARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDK
TQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGE
IFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDAN
LNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSL
GVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLV
LDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPLNEDV
LLAMEDMGLDKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKTLRLGALPS
MPRALAFQAPVNIQAEQAGTAMNISVPQVQLINPENQIVEPDGTLNLDSD
EGEEPSPEALVRYLSMRRHTVGVADPRTEVMEDLQKLLPGFPGVNPQAPF
LQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRR
ASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQE
AVQRYLANRSKRHTLAMTNPTAEIPPDLQRQLGQQPFRSRVWPPHLVPDQ
HRSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNNSSIKQL
QQECEQLQKMYGGQIDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPP
QPSPPLQAACENQPALLTHQLQRLRIQPSSPPPNHPNNHLFRQPSNSPPP
MSSAMIQPHGAASSSQFQGLPSRSAIFQQQPENCSSPPNVALTCLGMQQP
AQSQQVTIQVQEPVDMLSNMPGTAAGSSGRGISISPSAGQMQMQHRTNLM
ATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQSRG
SPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYTTSALQQAL
LSPTPPDYTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQ
QRQQQQQQQQQQEYQELFRHMNQGDAGSLAPSLGGQSMTERQALSYQNAD
SYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESMEEDCSC
EGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPESLLGTVSHAQELGI
HPYGHQPTAAFSKNKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYPARP
SVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALSSARMSDAV
LSQSSLMGSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCIT
DILLSYKHPEVSFSMEQAGVAAALEVLFQGPMSPILGYWKIKGLVQPTRL
LLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSM
AIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLK
VDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMC
LDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKS
D*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU46256
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: The sequence of this clone refers to an older reference sequence with an additional 59 amino acids at the N terminus.