cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pBABE H GFP TTBK2 KD
• Parent Plasmid: pBabeD hygro GFP
• Expressed: GFP-TTBK2 KD
• Genbank: NM_173500.3
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGTGGGGGAGGAGAGCAGCCGGATATCCTGAGTGTTGGAATCCTAGTGAAAGAAAGATGGAAAGTGTTGAGAAAGATTGGGGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAAAATGTTGCACTGAAGGTGGAATCAGCTCAACAACCAAAACAAGTTCTGAAAATGGAAGTTGCTGTTTTGAAAAAGCTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGGAATGATCGATTCAACTATGTGGTCATGCAGTTGCAGGGTCGGAATCTGGCAGATCTTCGCCGTAGCCAGTCCCGAGGCACATTCACCATTAGTACCACTCTCCGGCTGGGTAGACAGATTTTGGAGTCTATTGAAAGCATTCATTCTGTGGGATTCTTGCATCGAGACATCAAACCGTCGAACTTCGCTATGGGTCGCTTTCCTAGTACATGTAGGAAATGTTACATGCTTGCTTTTGGCTTGGCTCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTCGAGCTGTGGCAGGTTTTCGAGGGACAGTTCGTTATGCATCAATCAACGCACATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCCTTATTCTACATGTTGGTGGAGTTTGTGGTTGGTCAGCTGCCCTGGAGAAAAATAAAGGACAAGGAGCAAGTAGGCTCTATTAAGGAGAGATATGACCACAGGCTCATGTTGAAACATCTCCCTCCAGAATTCAGCATCTTTCTAGACCATATCTCTTCTTTGGATTATTTTACAAAACCAGACTACCAGCTTCTTACATCCGTGTTTGACAATAGCATCAAGACTTTTGGAGTAATTGAGAGTGACCCTTTTGACTGGGAGAAGACTGGAAATGATGGCTCCCTAACAACCACCACTACTTCTACCACCCCTCAGTTGCACACTCGCTTGACCCCTGCTGCAATTGGAATTGCCAATGCTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATACAGATGAGGTATTTCCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCCCTGTTGGTGTGTCACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGTCCCCAGGAGAAGGATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGATAAAGCTTGGAATTTGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCCAGGCAAATGGTCTTCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTCCGCTCAGAGATTACTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTTACGTTCCATTCACAGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAAAGCCAGACACTGACAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAAGATACCAGTGCAGGAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCCTTGCGTTCCTGCTGTGTCCCGTACTGACCACATCTGGCACTATGATGAAGAATATCTTCCAGATGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAGGCAGATGGTGGTGGCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTGCAAGCAAGAAATTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGGACCTTCAGGATTTTAGGACAAATGAGGCTGTAGGACATAAAACAACTGGAAGTCCTTCTGATGAGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACCTCAAGATGAAAAGCTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTCAGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAGAACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTTATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCTTACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTTCAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCCATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTTGGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGGGGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAAAGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAGACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAAGCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAGCATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAAGGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTTTTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTGACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGAAGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGGATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCTACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGGAGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGAAGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTTTTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGCAAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGCTTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGTGCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTTTTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTCACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGTCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCAGGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAAAGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTCCTAGGGAGTAGTAACTCCGACTCAGACCTTTTCTCCCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAACCCCGGAGCACTACTCAGTGCAAGAGTCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTCCCTCGCAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGCACGTCTAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTTCGTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCTTCCTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATGGCCTGAAAGGATCCGGCAGCCTCCACCACCACTCAGCCAGCACTAAAACCCCCCAAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGATAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSEFEVD
SLESAAAMSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLT
RENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVV
MQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIK
PSNFAMGRFPSTCRKCYMLAFGLARQFTNSCGDVRPPRAVAGFRGTVRYA
SINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYD
HRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESD
PFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENT
DEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDANKNKI
KLGICKAATEEENSHGQANGLLNAPSLGSPIRVRSEITQPDRDIPLVRKL
RSIHSFELEKRLTLEPKPDTDKFLETCLEKMQKDTSAGKESILPALLHKP
CVPAVSRTDHIWHYDEEYLPDASKPASANTPEQADGGGSNGFIAVNLSSC
KQEIDSKEWVIVDKEQDLQDFRTNEAVGHKTTGSPSDEEPEVLQVLEASP
QDEKLQLGPWAENDHLKKETSGVVLALSAEGPPTAASEQYTDRLELQPGA
ASQFIAATPTSLMEAQAEGPLTAITIPRPSVASTQSTSGSFHCGQQPEKK
DLQPMEPTVELYSPRENFSGLVVTEGEPPSGGSRTDLGLQIDHIGHDMLP
NIRESNKSQDLGPKELPDHNRLVVREFENLPGETEEKSILLESDNEDEKL
SRGQHCIEISSLPGDLVIVEKDHSATTEPLDVTKTQTFSVVPNQDKNNEI
MKLLTVGTSEISSRDIDPHVEGQIGQVAEMQKNKISKDDDIMSEDLPGHQ
GDLSTFLHQEGKREKITPRNGELFHCVSENEHGAPTRKDMVRSSFVTRHS
RIPVLAQEIDSTLESSSPVSAKEKLLQKKAYQPDLVKLLVEKRQFKSFLG
DLSSASDKLLEEKLATVPAPFCEEEVLTPFSRLTVDSHLSRSAEDSFLSP
IISQSRKSKIPRPVSWVNTDQVNSSTSSQFFPRPPPGKPPTRPGVEARLR
RYKVLGSSNSDSDLFSRLAQILQNGSQKPRSTTQCKSPGSPHNPKTPPKS
PVVPRRSPSASPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPPS
HSGSSSSRRSCQQEHCKPSKNGLKGSGSLHHHSASTKTPQGKSKPASKLS
R*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44924
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kinase Dead