cDNA Clone OTUD6A

• Synonyms: DUBA2; HSHIN6
• Plasmid: pcDNA5 FRT/TO 3XFLAG OTUD6A
• Parent Plasmid: pcDNA5 FRT/TO 3FLAG1
• Expressed: 3XFLAG-OTUD6A
• Genbank: NM_207320.1 GI:46409281
• Tag: 3FLAG
• Species: Human

Sequence of Insert

GGATCCATGGATGATCCGAAGAGTGAACAGCAGCGCATACTGCGCCGCCA
CCAACGCGAGAGGCAGGAGCTGCAGGCCCAGATCCGGAGCTTAAAAAACT
CGGTCCCCAAGACCGACAAGACGAAAAGAAAGCAGTTGCTCCAAGACGTG
GCCCGCATGGAGGCCGAGATGGCTCAGAAGCACCGGCAGGAGCTGGAGAA
GTTCCAAGACGACAGTAGCATTGAATCTGTCGTCGAAGACCTGGCCAAGA
TGAATCTGGAAAACCGGCCTCCCCGCTCCTCCAAAGCCCACAGAAAGAGA
GAAAGAATGGAGTCCGAGGAGAGGGAGCGCCAGGAGAGCATCTTCCAGGC
TGAGATGTCGGAGCACCTGGCCGGCTTCAAGCGCGAGGAGGAGGAGAAGC
TCGCCGCCATCCTGGGAGCCAGGGGTCTGGAGATGAAAGCGATCCCGGCC
GACGGCCACTGCATGTACCGCGCCATCCAAGACCAGCTGGTGTTCAGCGT
GTCTGTGGAGATGCTGCGCTGCCGCACCGCCAGCTACATGAAGAAGCACG
TCGACGAGTTCCTGCCCTTCTTCAGCAACCCCGAGACCAGCGACTCCTTC
GGCTACGACGACTTCATGATCTACTGCGACAACATCGTGCGCACCACGGC
ATGGGGAGGCCAGCTGGAGCTGAGGGCCCTGTCGCACGTCCTGAAGACCC
CCATCGAGGTGATCCAGGCCGACTCGCCCACCTTGATCATCGGGGAGGAG
TACGTCAAGAAGCCGATCATCCTGGTCTACCTGCGCTATGCCTACAGCCT
CGGCGAGCACTACAACTCCGTGACACCGCTCGAGGCCGGCGCCGCCGGGG
GCGTGCTCCCGCGTCTCCTGTAGGCGGCGCGCAAGGGCGAATTCCACATT
GGTCGCTGCAGCCCGGGGGATCCCCGGAATTCCCGGGTCGACTCGAGCGG
CCGC

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSMDDPKSEQQRILRRHQRERQE
LQAQIRSLKNSVPKTDKTKRKQLLQDVARMEAEMAQKHRQELEKFQDDSS
IESVVEDLAKMNLENRPPRSSKAHRKRERMESEERERQESIFQAEMSEHL
AGFKREEEEKLAAILGARGLEMKAIPADGHCMYRAIQDQLVFSVSVEMLR
CRTASYMKKHVDEFLPFFSNPETSDSFGYDDFMIYCDNIVRTTAWGGQLE
LRALSHVLKTPIEVIQADSPTLIIGEEYVKKPIILVYLRYAYSLGEHYNS
VTPLEAGAAGGVLPRLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45983
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Intentional NotI immediately after stop in 20335 not intact. ; Warning: BamHI between stop and 3' NotI; If subcloning from this clone use BamHI-BamHI