cDNA Clone SYT1 (rat)

• Synonyms: P65, synaptotagmin 1, synaptotagmin-1
• Plasmid: pcDNA hSYN1p Phluorin rSYT1 K325A
• Parent Plasmid: pcDNA3.1+(neo)
• Expressed: Signal peptide-Phluorin-rSYT1 K325A
• Genbank: NM_001033680.2
• Species: Rat

Sequence of Insert

CCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCTAGACTCGAGCTGCAGAGGGCCCTGCGTATGAGTGCAAGTGGGTTTTAGGACCAGGATGAGGCGGGGTGGGGGTGCCTACCTGACGACCGACCCCGACCCACTGGACAAGCACCCAACCCCCATTCCCCAAATTGCGCATCCCCTATCAGAGAGGGGGAGGGGAAACAGGATGCGGCGAGGCGCGTGCGCACTGCCAGCTTCAGCACCGCGGACAGTGCCTTCGCCCCCGCCTGGCGGCGCGCGCCACCGCCGCCTCAGCACTGAAGGCGCGCTGACGTCACTCGCCGGTCCCCCGCAAACTCCCCTTCCCGGCCACCTTGGTCGCGTCCGCGCCGCCGCCGGCCCAGCCGGACCGCACCACGCGAGGCGCGAGATAGGGGGGCACGGGCGCGACCATCTGCGCTGCGGCGCCGGCGACTCAGCGCTGCCTCAGTCTGCGGTGGGCAGCGGAGGAGTCGTGTCGTGCCTGAGAGCGCAGTCGAATTAGCTTGGTACCGAGCTCGGATCCATGAAAATCCTCGTGGCGGTGGCGGTCTTTTTTCTCGTTTCCACTCAACTGTTTGCAGAGGAAATCGGTGCCAACAAGCTTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCTTGGCCAACACTTGTCACTACTTTAACTTATGGTGTTCAATGCTTTTCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACGATCACCAGGTGTACATCATGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATTAGACACAACATTGAAGATGGAGGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGGCCCGTCCTTTTACCAGACAACCATTACCTGTTTACAACTTCTACTCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAACCGGTGACTACGACATCCCGACTACGCTCGAGAACCTGTACTTCCAGGGTGAGCTCAAGACTGTTGATGCTGACATGGTGAGTGCCAGTCATCCTGAGGCCCTGGCCGCCCCTGTCACCACTGTTGCGACCCTTGTCCCACACAATGCCACTGAGCCAGCCAGTCCTGGGGAAGGGAAGGAAGATGCCTTTTCCAAGCTGAAGCAGAAGTTTATGAATGAGCTGCATAAAATTCCATTGCCACCGTGGGCCTTAATAGCCATAGCCATAGTTGCGGTCCTTTTAGTCGTAACCTGCTGCTTTTGTGTCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGGAAGGAAAAGGGAGGAAAGAACGCCATTAACATGAAAGACGTGAAAGACTTAGGGAAGACCATGAAGGATCAGGCCCTTAAGGATGACGATGCTGAAACCGGACTGACTGATGGAGAAGAAAAGGAAGAGCCCAAGGAAGAGGAGAAACTGGGAAAGCTCCAATATTCACTGGACTATGACTTCCAGAATAACCAGCTGTTGGTGGGAATCATCCAGGCTGCTGAACTGCCCGCCCTGGACATGGGGGGTACATCCGATCCATACGTCAAAGTCTTCCTGCTGCCTGACAAAAAGAAGAAATTTGAGACTAAAGTCCACCGGAAAACCCTCAATCCAGTCTTCAATGAACAATTTACTTTCAAGGTACCCTACTCGGAATTAGGTGGCAAAACCCTGGTGATGGCTGTGTATGACTTTGATCGCTTCTCCAAGCACGACATCATCGGAGAGTTCAAAGTTCCTATGAACACCGTGGATTTTGGCCATGTGACCGAGGAGTGGCGCGATCTCCAGAGCGCTGAGAAAGAAGAGCAAGAGAAACTGGGTGACATCTGCTTCTCCCTCCGCTACGTCCCTACTGCCGGCAAACTGACTGTTGTCATTCTGGAAGCCAAGAACCTGAAGAAGATGGATGTGGGTGGCTTATCTGATCCCTACGTGAAGATTCACCTGATGCAGAACGGTAAGAGGCTGAAGgcGAAAAAGACGACGATTAAGAAGAACACACTCAACCCCTACTACAACGAGTCCTTCAGCTTTGAAGTTCCGTTCGAGCAAATCCAGAAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGACGCGATCGGCAAAGTCTTCGTTGGTTACAACAGCACTGGGGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCCGGCGACCCATCGCACAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTGATGCCATGCTGGCTGTCAAGAAGTAAGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG

Amino Acid Sequence

MKILVAVAVFFLVSTQLFAEEIGANKLSKGEELFTGVVPILVELDGDVNG
HKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRY
PDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
LKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANFKIRHNIEDG
GVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFV
TAAGITHGMDELYKTGDYDIPTTLENLYFQGELKTVDADMVSASHPEALA
APVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALI
AIAIVAVLLVVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGK
TMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVG
IIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFT
FKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRD
LQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPY
VKIHLMQNGKRLKAKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTV
LDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEE
EVDAMLAVKK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU44969
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: DU42404 [K325A]. Complete cassette including hSYN1 promoter and BGH polyA shown as sequence of insert.