cDNA Clone Parkin

• Synonyms: park2
• Plasmid: pET6HIS SUMO Parkin S65TAG C431F
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO Parkin S65TAG C431F
• Genbank: AB009973.1
• Tag: 6His
• Tag: SUMO
• Species: Human

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFA
GKELRNDWTVQNCDLDQQ*IVHIVQRPWRKGQEMNATGGDDPRNAAGGCE
REPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSF
YVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGEC
QSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRS
PVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNS
LIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPD
QRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTTTQAYRVDER
AAEQARWEAASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCRLEWC
WNCGCEWNRVCMGDHWFDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46259
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Full-length Parkin, C431F mutant. Please make with amber codon at position serine 65, and with His-SUMO tag in pET6His-SUMO vector. Please also check His-SUMO for any additional TAG codons. Thanks, Helen