cDNA Clone Traf3IP2

• Plasmid: pGEX6P-1 Traf3IP2
• Parent Plasmid: pGEX6P1
• Expressed: GST Traf3IP2
• Genbank: EAW48285.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCTCCTCAGCTTCAAGAAACTAGAATGAACCGAAGCATTCC
TGTGGAGGTTGATGAATCAGAACCATACCCAAGTCAGTTGCTGAAACCAA
TCCCAGAATATTCCCCGGAAGAGGAATCAGAACCACCTGCTCCAAATATA
AGGAACATGGCACCCAACAGCTTGTCTGCACCCACAATGCTTCACAATTC
CTCCGGAGACTTTTCTCAAGCTCACTCAACCCTGAAACTTGCAAATCACC
AGCGGCCTGTATCCCGGCAGGTCACCTGCCTGCGCACTCAAGTTCTGGAG
GACAGTGAAGACAGTTTCTGCAGGAGACACCCAGGCCTGGGCAAAGCTTT
CCCTTCTGGGTGCTCTGCAGTCAGCGAGCCTGCGTCTGAGTCTGTGGTTG
GAGCCCTCCCTGCAGAGCATCAGTTTTCATTTATGGAAAAACGTAATCAA
TGGCTGGTATCTCAGCTTTCAGCGGCTTCTCCTGACACTGGCCATGACTC
AGACAAATCAGACCAAAGTTTACCTAATGCCTCAGCAGACTCCTTGGGCG
GTAGCCAGGAGATGGTGCAACGGCCCCAGCCTCACAGGAACCGAGCAGGC
CTGGATCTGCCAACCATAGACACGGGATATGATTCCCAGCCCCAGGATGT
CCTGGGCATCAGGCAGCTGGAAAGGCCCCTGCCCCTCACCTCCGTGTGTT
ACCCCCAGGACCTCCCCAGACCTCTCAGGTCCAGGGAGTTCCCTCAGTTT
GAACCTCAGAGGTATCCAGCATGTGCACAGATGCTGCCTCCCAATCTTTC
CCCACATGCTCCATGGAACTATCATTACCATTGTCCTGGAAGTCCCGATC
ACCAGGTGCCATATGGCCATGACTACCCTCGAGCAGCCTACCAGCAAGTG
ATCCAGCCGGCTCTGCCTGGGCAGCCCCTGCCTGGAGCCAGTGTGAGAGG
CCTGCACCCTGTGCAGAAGGTTATCCTGAATTATCCCAGCCCCTGGGACC
ACGAAGAGAGGCCCGCACAGAGAGACTGCTCCTTTCCGGGGCTTCCAAGG
CACCAGGACCAGCCACATCACCAGCCACCTAATAGAGCTGGTGCTCCTGG
GGAGTCCTTGGAGTGCCCTGCAGAGCTGAGACCACAGGTTCCCCAGCCTC
CGTCCCCAGCTGCTGTGCCTAGACCCCCTAGCAACCCTCCAGCCAGAGGA
ACTCTAAAAACAAGCAATTTGCCAGAAGAATTGCGGAAAGTCTTTATCAC
TTATTCGATGGACACAGCTATGGAGGTGGTGAAATTCGTGAACTTTTTGT
TGGTAAATGGCTTCCAAACTGCAATTGACATATTTGAGGATAGAATCCGA
GGCATTGATATCATTAAATGGATGGAGCGCTACCTTAGGGATAAGACCGT
GATGATAATCGTAGCAATCAGCCCCAAATACAAACAGGACGTGGAAGGCG
CTGAGTCGCAGCTGGACGAGGATGAGCATGGCTTACATACTAAGTACATT
CATCGAATGATGCAGATTGAGTTCATAAAACAAGGAAGCATGAATTTCAG
ATTCATCCCTGTGCTCTTCCCAAATGCTAAGAAGGAGCATGTGCCCACCT
GGCTTCAGAACACTCATGTCTACAGCTGGCCCAAGAATAAAAAAAACATC
CTGCTGCGGCTGCTGAGAGAGGAAGAGTATGTGGCTCCTCCACGGGGGCC
TCTGCCCACCCTTCAGGTGGTTCCCTTGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPPQLQETRMNRSIPVEVD
ESEPYPSQLLKPIPEYSPEEESEPPAPNIRNMAPNSLSAPTMLHNSSGDF
SQAHSTLKLANHQRPVSRQVTCLRTQVLEDSEDSFCRRHPGLGKAFPSGC
SAVSEPASESVVGALPAEHQFSFMEKRNQWLVSQLSAASPDTGHDSDKSD
QSLPNASADSLGGSQEMVQRPQPHRNRAGLDLPTIDTGYDSQPQDVLGIR
QLERPLPLTSVCYPQDLPRPLRSREFPQFEPQRYPACAQMLPPNLSPHAP
WNYHYHCPGSPDHQVPYGHDYPRAAYQQVIQPALPGQPLPGASVRGLHPV
QKVILNYPSPWDHEERPAQRDCSFPGLPRHQDQPHHQPPNRAGAPGESLE
CPAELRPQVPQPPSPAAVPRPPSNPPARGTLKTSNLPEELRKVFITYSMD
TAMEVVKFVNFLLVNGFQTAIDIFEDRIRGIDIIKWMERYLRDKTVMIIV
AISPKYKQDVEGAESQLDEDEHGLHTKYIHRMMQIEFIKQGSMNFRFIPV
LFPNAKKEHVPTWLQNTHVYSWPKNKKNILLRLLREEEYVAPPRGPLPTL
QVVPL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46209
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Agrees with NCBI reference but lab using DU46199 with H332Q snp as this was isolated from 6 sequences RT PCR amplified from 3 different RNA sources