cDNA Clone Traf3IP2

• Plasmid: pRETROX Tight HA Traf3IP2 V319R H332Q
• Parent Plasmid: pRetroX tight puro
• Expressed: HA Traf3IP2 V319R H332Q
• Genbank: EAW48285.1
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCCTCCTCAGCTTCAAGAAACTAGAATGAACCGAAGCATTCC
TGTGGAGGTTGATGAATCAGAACCATACCCAAGTCAGTTGCTGAAACCAA
TCCCAGAATATTCCCCGGAAGAGGAATCAGAACCACCTGCTCCAAATATA
AGGAACATGGCACCCAACAGCTTGTCTGCACCCACAATGCTTCACAATTC
CTCCGGAGACTTTTCTCAAGCTCACTCAACCCTGAAACTTGCAAATCACC
AGCGGCCTGTATCCCGGCAGGTCACCTGCCTGCGCACTCAAGTTCTGGAG
GACAGTGAAGACAGTTTCTGCAGGAGACACCCAGGCCTGGGCAAAGCTTT
CCCTTCTGGGTGCTCTGCAGTCAGCGAGCCTGCGTCTGAGTCTGTGGTTG
GAGCCCTCCCTGCAGAGCATCAGTTTTCATTTATGGAAAAACGTAATCAA
TGGCTGGTATCTCAGCTTTCAGCGGCTTCTCCTGACACTGGCCATGACTC
AGACAAATCAGACCAAAGTTTACCTAATGCCTCAGCAGACTCCTTGGGCG
GTAGCCAGGAGATGGTGCAACGGCCCCAGCCTCACAGGAACCGAGCAGGC
CTGGATCTGCCAACCATAGACACGGGATATGATTCCCAGCCCCAGGATGT
CCTGGGCATCAGGCAGCTGGAAAGGCCCCTGCCCCTCACCTCCGTGTGTT
ACCCCCAGGACCTCCCCAGACCTCTCAGGTCCAGGGAGTTCCCTCAGTTT
GAACCTCAGAGGTATCCAGCATGTGCACAGATGCTGCCTCCCAATCTTTC
CCCACATGCTCCATGGAACTATCATTACCATTGTCCTGGAAGTCCCGATC
ACCAGGTGCCATATGGCCATGACTACCCTCGAGCAGCCTACCAGCAAGTG
ATCCAGCCGGCTCTGCCTGGGCAGCCCCTGCCTGGAGCCAGTGTGAGAGG
CCTGCACCCTAGGCAGAAGGTTATCCTGAATTATCCCAGCCCCTGGGACC
AAGAAGAGAGGCCCGCACAGAGAGACTGCTCCTTTCCGGGGCTTCCAAGG
CACCAGGACCAGCCACATCACCAGCCACCTAATAGAGCTGGTGCTCCTGG
GGAGTCCTTGGAGTGCCCTGCAGAGCTGAGACCACAGGTTCCCCAGCCTC
CGTCCCCAGCTGCTGTGCCTAGACCCCCTAGCAACCCTCCAGCCAGAGGA
ACTCTAAAAACAAGCAATTTGCCAGAAGAATTGCGGAAAGTCTTTATCAC
TTATTCGATGGACACAGCTATGGAGGTGGTGAAATTCGTGAACTTTTTGT
TGGTAAATGGCTTCCAAACTGCAATTGACATATTTGAGGATAGAATCCGA
GGCATTGATATCATTAAATGGATGGAGCGCTACCTTAGGGATAAGACCGT
GATGATAATCGTAGCAATCAGCCCCAAATACAAACAGGACGTGGAAGGCG
CTGAGTCGCAGCTGGACGAGGATGAGCATGGCTTACATACTAAGTACATT
CATCGAATGATGCAGATTGAGTTCATAAAACAAGGAAGCATGAATTTCAG
ATTCATCCCTGTGCTCTTCCCAAATGCTAAGAAGGAGCATGTGCCCACCT
GGCTTCAGAACACTCATGTCTACAGCTGGCCCAAGAATAAAAAAAACATC
CTGCTGCGGCTGCTGAGAGAGGAAGAGTATGTGGCTCCTCCACGGGGGCC
TCTGCCCACCCTTCAGGTGGTTCCCTTGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMPPQLQETRMNRSIPVEVDESEPYPSQLLKPIPEYSPE
EESEPPAPNIRNMAPNSLSAPTMLHNSSGDFSQAHSTLKLANHQRPVSRQ
VTCLRTQVLEDSEDSFCRRHPGLGKAFPSGCSAVSEPASESVVGALPAEH
QFSFMEKRNQWLVSQLSAASPDTGHDSDKSDQSLPNASADSLGGSQEMVQ
RPQPHRNRAGLDLPTIDTGYDSQPQDVLGIRQLERPLPLTSVCYPQDLPR
PLRSREFPQFEPQRYPACAQMLPPNLSPHAPWNYHYHCPGSPDHQVPYGH
DYPRAAYQQVIQPALPGQPLPGASVRGLHPrQKVILNYPSPWDQEERPAQ
RDCSFPGLPRHQDQPHHQPPNRAGAPGESLECPAELRPQVPQPPSPAAVP
RPPSNPPARGTLKTSNLPEELRKVFITYSMDTAMEVVKFVNFLLVNGFQT
AIDIFEDRIRGIDIIKWMERYLRDKTVMIIVAISPKYKQDVEGAESQLDE
DEHGLHTKYIHRMMQIEFIKQGSMNFRFIPVLFPNAKKEHVPTWLQNTHV
YSWPKNKKNILLRLLREEEYVAPPRGPLPTLQVVPL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46246
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has H332Q which is a naturally occuring varient (http://www.uniprot.org/uniprot/O43734) and is benign according to ENSMBL (http://www.ensembl.org/Homo_sapiens/Gene/Variation_Gene/Table?db=core;g=ENSG00000056972;r=6:111877657-111927481#missense_variant_tablePanel)