cDNA Clone PLK1

• Synonyms: PLK; STPK13
• Plasmid: pcDNA5 FRT/TO HA2 PLK1 H538A K540M
• Parent Plasmid: pcDNA5 FRT/TO HA2
• Expressed: HA-PLK1 H538A K540M
• Genbank: NM_005030.3 GI:34147632
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGTGCTGCAGTGACTGCAGGGAAGCTGGCACGGGCACC
GGCCGACCCTGGGAAAGCCGGGGTCCCCGGAGTTGCAGCTCCCGGAGCTC
CGGCGGCGGCTCCACCGGCGAAAGAGATCCCGGAGGTCCTAGTGGACCCA
CGCAGCCGGCGGCGCTATGTGCGGGGCCGCTTTTTGGGCAAGGGCGGCTT
TGCCAAGTGCTTCGAGATCTCGGACGCGGACACCAAGGAGGTGTTCGCGG
GCAAGATTGTGCCTAAGTCTCTGCTGCTCAAGCCGCACCAGAGGGAGAAG
ATGTCCATGGAAATATCCATTCACCGCAGCCTCGCCCACCAGCACGTCGT
AGGATTCCACGGCTTTTTCGAGGACAACGACTTCGTGTTCGTGGTGTTGG
AGCTCTGCCGCCGGAGGTCTCTCCTGGAGCTGCACAAGAGGAGGAAAGCC
CTGACTGAGCCTGAGGCCCGATACTACCTACGGCAAATTGTGCTTGGCTG
CCAGTACCTGCACCGAAACCGAGTTATTCATCGAGACCTCAAGCTGGGCA
ACCTTTTCCTGAATGAAGATCTGGAGGTGAAAATAGGGGATTTTGGACTG
GCAACCAAAGTCGAATATGACGGGGAGAGGAAGAAGACCCTGTGTGGGAC
TCCTAATTACATAGCTCCCGAGGTGCTGAGCAAGAAAGGGCACAGTTTCG
AGGTGGATGTGTGGTCCATTGGGTGTATCATGTATACCTTGTTAGTGGGC
AAACCACCTTTTGAGACTTCTTGCCTAAAAGAGACCTACCTCCGGATCAA
GAAGAATGAATACAGTATTCCCAAGCACATCAACCCCGTGGCCGCCTCCC
TCATCCAGAAGATGCTTCAGACAGATCCCACTGCCCGCCCAACCATTAAC
GAGCTGCTTAATGACGAGTTCTTTACTTCTGGCTATATCCCTGCCCGTCT
CCCCATCACCTGCCTGACCATTCCACCAAGGTTTTCGATTGCTCCCAGCA
GCCTGGACCCCAGCAACCGGAAGCCCCTCACAGTCCTCAATAAAGGCTTG
GAGAACCCCCTGCCTGAGCGTCCCCGGGAAAAAGAAGAACCAGTGGTTCG
AGAGACAGGTGAGGTGGTCGACTGCCACCTCAGTGACATGCTGCAGCAGC
TGCACAGTGTCAATGCCTCCAAGCCCTCGGAGCGTGGGCTGGTCAGGCAA
GAGGAGGCTGAGGATCCTGCCTGCATCCCCATCTTCTGGGTCAGCAAGTG
GGTGGACTATTCGGACAAGTACGGCCTTGGGTATCAGCTCTGTGATAACA
GCGTGGGGGTGCTCTTCAATGACTCAACACGCCTCATCCTCTACAATGAT
GGTGACAGCCTGCAGTACATAGAGCGTGACGGCACTGAGTCCTACCTCAC
CGTGAGTTCCCATCCCAACTCCTTGATGAAGAAGATCACCCTCCTTAAAT
ATTTCCGCAATTACATGAGCGAGCACTTGCTGAAGGCAGGTGCCAACATC
ACGCCGCGCGAAGGTGATGAGCTCGCCCGGCTGCCCTACCTACGGACCTG
GTTCCGCACCCGCAGCGCCATCATCCTGCACCTCAGCAACGGCAGCGTGC
AGATCAACTTCTTCCAGGATGCCACCATGCTCATCTTGTGCCCACTGATG
GCAGCCGTGACCTACATCGACGAGAAGCGGGACTTCCGCACATACCGCCT
GAGTCTCCTGGAGGAGTACGGCTGCTGCAAGGAGCTGGCCAGCCGGCTCC
GCTACGCCCGCACTATGGTGGACAAGCTGCTGAGCTCACGCTCGGCCAGC
AACCGTCTCAAGGCCTCCTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMSAAVTAGKLARAPADPGKAGVPGVAA
PGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKE
VFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVF
VVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDL
KLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKG
HSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPV
AASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSI
APSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDM
LQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQL
CDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKIT
LLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSN
GSVQINFFQDATMLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELA
SRLRYARTMVDKLLSSRSASNRLKAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49064
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee