cDNA Clone WNK1

• Synonyms: HSAN2, HSN2, KDP, PRKWNK1, PSK, p65
• Plasmid: pSCB WNK1 tv3 P3 Pvu Sal
• Parent Plasmid: pSC-B
• Genbank: NM_018979.3
• Species: Human

Sequence of Insert

CGATCGACTCAGGGAGTCTCTCAGGTTGCTCCTGCAGAGCCAGTTGCAGTAGCACAGCCCCAAGCTACCCAGCCGACCACTTTGGCTTCCTCTGTAGACAGTGCACATTCAGATGTTGCTTCAGGTATGAGTGATGGCAATGAGAACGTCCCATCTTCCAGTGGAAGGCATGAAGGAAGAACTACAAAACGGCATTACCGAAAATCTGTAAGGAGTCGCTCTCGACATGAAAAAACTTCACGCCCAAAATTAAGAATTTTGAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACTCATAATAGGAAAATGGTTACATTCAAATTTGACCTAGATGGTGACAACCCCGAGGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCGTTTGTGGATCAAGTGCGAGAAATTATTGAAAAAGCTGATGAAATGCTCAGTGAGGATGTCAGTGTGGAACCAGAGGGTGATCAGGGATTGGAGAGTCTACAAGGAAAGGATGACTATGGCTTTTCAGGTTCTCAGAAGCTTGAAGGAGAGTTCAAACAACCAATTCCTGCGTCTTCCATGCCACAGCAAATAGGCATTCCTACCAGTTCTTTAACTCAAGTTGTTCATTCTGCGGGAAGGCGGTTTATAGTGAGTCCTGTGCCAGAAAGCCGATTACGAGAATCAAAAGTTTTCCCCAGTGAAATAACAGATACAGTTGCTGCCTCTACAGCTCAGAGCCCTGGAATGAACTTGTCTCACTCTGCATCATCCCTTAGTCTACAACAGGCCTTTTCTGAACTTAGACGTGCCCAAATGACAGAAGGACCCAACACAGCACCTCCAAACTTTAGTCATACAGGACCAACATTTCCAGTAGTACCTCCTTTCTTAAGTAGCATTGCTGGAGTCCCAACCACAGCAGCAGCCACAGCACCAGTCCCTGCAACAAGCAGCCCTCCTAATGACATTTCCACATCAGTAATTCAGTCTGAGGTTACAGTGCCCACTGAAGAGGGGATTGCTGGAGTTGCCACCAGCACAGGTGTGGTAACTTCGGGTGGTCTCCCCATACCACCTGTGTCTGAATCACCAGTACTTTCCAGCGTAGTTTCAAGTATCACAATACCTGCAGTTGTCTCAATATCTACTACATCCCCGTCACTTCAAGTCCCCACATCCACATCTGAGATCGTTGTTTCTAGTACAGCACTGTATCCTTCAGTAACAGTTTCAGCAACTTCAGCCTCTGCAGGGGGCAGTACTGCTACCCCAGGTCCTAAGCCTCCAGCTGTAGTATCTCAGCAGGCAGCAGGCAGCACTACTGTGGGAGCCACATTAACATCAGTTTCTACCACCACTTCATTCCCAAGCACAGCTTCACAGCTGTCCATTCAACTTAGCAGCAGTACTTCTACTCCTACTTTAGCTGAAACCGTGGTAGTTAGCGCACACTCACTAGATAAGACATCTCATAGCAGTACAACTGGATTGGCTTTCTCCCTCTCTGCACCATCTTCCTCTTCCTCTCCTGGAGCAGGAGTGTCTAGTTATATTTCTCAGCCTGGTGGGCTGCATCCTTTGGTCATTCCATCAGTGATAGCTTCTACTCCTATTCTTCCCCAAGCAGCAGGACCTACTTCTACACCTTTATTACCCCAAGTACCTAGTATCCCACCCTTGGTACAGCCTGTTGCCAATGTGCCTGCTGTACAGCAGACACTAATTCATAGTCAGCCTCAACCAGCTTTGCTTCCCAACCAGCCCCATACTCATTGTCCTGAAGTAGATTCTGATACACAACCCAAAGCTCCTGGAATTGATGACATAAAGACTCTAGAAGAAAAGCTGCGGTCTCTGTTCAGTGAACACAGCTCATCTGGAGCTCAGCATGCCTCTGTCTCACTGGAGACCTCACTAGTCATAGAGAGCACTGTCACACCAGGCATCCCAACTACTGCTGTTGCACCAAGCAAACTCCTGACTTCTACCACAAGTACTTGCTTACCACCAACCAATTTACCACTAGGAACAGTTGCTTTGCCAGTTACACCAGTGGTCACACCTGGGCAAGTTTCTACCCCAGTCAGCACTACTACATCAGGAGTGAAACCTGGAACTGCTCCCTCCAAGCCACCTCTAACTAAGGCTCCGGTGCTGCCAGTGGGTACTGAACTTCCAGCAGGTACTCTACCCAGCGAGCAGCTGCCACCTTTTCCAGGACCTTCTCTAACCCAGTCCCAGCAACCTCTAGAGGATCTTGATGCTCAATTGAGAAGAACACTTAGTCCAGAGATTATCACAGTGACTTCTGCGGTTGGTCCTGTGTCCATGGCGGCTCCAACAGCAATCACAGAAGCAGGAACACAGCCTCAGAAGGGTGTTTCTCAAGTCAAAGAAGGCCCTGTCCTAGCGACTAGTTCAGGAGCTGGTGTTTTTAAGATGGGACGATTTCAGGTTTCTGTTGCAGCAGACGGTGCCCAGAAAGAGGGTAAAAATAAGTCAGAAGATGCAAAGTCTGTTCATTTTGAATCCAGCACCTCAGAGTCCTCAGTGCTATCAAGTAGTAGTCCAGAGAGTACCTTGGTGAAACCAGAGCCGAATGGCATAACCATCCCTGGTATCTCTTCAGATGTGCCAGAGAGTGCCCACAAAACTACTGCCTCAGAGGCAAAGTCAGACACTGGGCAGCCTACCAAGGTTGGACGTTTTCAGGTGACAACTACAGCAAACAAAGTGGGTCGTTTCTCTGTATCAAAAACTGAGGACAAGATCACTGACACAAAGAAAGAAGGACCAGTGGCATCTCCTCCTTTTATGGATTTGGAACAAGCTGTTCTTCCTGCTGTGATACCAAAGAAAGAGAAGCCTGAACTGTCAGAGCCTTCACATCTAAATGGGCCGTCTTCTGACCCGGAGGCCGCTTTTTTAAGTAGGGATGTGGATGATGGTTCCGGTAGTCCACACTCGCCCCATCAGCTGAGCTCAAAGAGCCTTCCTAGCCAGAATCTAAGTCAAAGCCTTAGTAATTCATTTAACTCCTCTTACATGAGTAGCGACAATGAGTCAGATATCGAAGATGAAGACTTAAAGTTAGAGCTGCGACGACTACGAGATAAACATCTCAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACCAAACTGGGCAAGGTGCCCCCTGCTGTTATTATTCCCCCAGCTGCTCCCCTTTCAGGGAGAAGACGACGACCCACTAAAAGCAAAGGCAGCAAATCTAGTCGAAGCAGTTCCTTGGGGAATAAAAGCCCCCAGCTTTCAGGTAACCTGTCTGGTCAGAGTGCAGCTTCAGTCTTGCACCCCCAGCAGACCCTCCACCCTCCTGGCAACATCCCAGAGTCCGGGCAGAATCAGCTGTTACAGCCCCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTCACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGAACCAGCAGCACAAACACTGTTGGGGCAACAGTGAACAGCCAAGCCGCCCAAGCTCAGCCTCCTGCCATGACGTCCAGCAGGAAGGGCACATTCACAGATGACTTGCACAAGTTGGTAGACAATTGGGCCCGAGATGCCATGAATCTCTCAGGCAGGAGAGGAAGCAAAGGGCACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCACCTGGGCAACTGTGCATCTCCATGACCTCGAACCTGGGTGGCTCTGCCCCCATCTCTGCAGCATCAGCTACCTCTCTAGGTCACTTCACCAAGTCTATGTGCCCCCCACAGCAGTATGGCTTTCCAGCTACCCCATTTGGCGCTCAATGGAGTGGGACGGGTGGCCCAGCACCACAGCCACTTGGCCAGTTCCAACCTGTGGGAACTGCCTCCTTGCAGAATTTCAACATCAGCAATTTGCAGAAATCCATCAGCAACCCCCCAGGCTCCAACCTGCGGACCACTTAGGTCGAC

Amino Acid Sequence

TQGVSQVAPAEPVAVAQPQATQPTTLASSVDSAHSDVASGMSDGNENVPS
SSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLE
THNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKA
DEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQ
QIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQ
SPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPP
FLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVAT
STGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTS
EIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGAT
LTSVSTTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTT
GLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGP
TSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEV
DSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTV
TPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPV
STTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQ
SQQPLEDLDAQLRRTLSPEIITVTSAVGPVSMAAPTAITEAGTQPQKGVS
QVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESS
TSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTG
QPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAV
LPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSS
KSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQ
DLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRS
SSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPS
PSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAM
TSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAP
GQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWS
GTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48060
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: WNK1 tv1 1039-end for construction of WNK1 variant 3, contains several variations as compared to the wild-type and will require repair. Grow at 30oC.