cDNA Clone SGK3

• Synonyms: CISK, SGKL
• Plasmid: pMK-RQ SGK3 C-ter GFP donor
• Parent Plasmid: pMK-RQ
• Genbank: ENSG00000104205
• Tag: GFP
• Species: Human

Sequence of Insert

TAAAGGACTAAATTGGGCCAGGCGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGAATCACAAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGATGAAACCCTGTCTGTACTAAAAATACAAAAAGTTAGCTGGGCGTGGTAGGTGGGCGCCTGTAATCCCAGCTACTCGGGAGGGTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCCAAGATCGCGCCACTGGCATGCCAGCCCAGGTGGCAGTGTGAGACTCTGTCTCAAATAAATAAATAAATAAAGGATTAAATTAATGAAAGAAAAGCTAAGGTGAATAATACTGTGCTTTTGATGTTTTAGGCTGGACCAGATGATATCAGAAACTTTGACACAGCATTTACAGAAGAAACAGTTCCATATTCTGTGTGTGTATCTTCTGACTATTCTATAGTGAATGCCAGTGTATTGGAaGCtGAcGAcGCgTTCGTTGGTTTCTCTTATGCACCTCCTTCAGAAGACTTATTTTTGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGAGCAGTTTGCCATTCAGAAACCATTGAGCAAAATAAGTCTATAGATGGGACTGAAACTTCTATTTGTGTGAATATATTCAAATATGTATAACTAGTGCCTCATTTTTATATGTAATGATGAAAACTATGAAAAAATGTATTTTCTTCTATGTGCAAGAAAAATAGGGCATTTCAAAGAGCTGTTTTGATTAAAATTTATATTCTTGTTTAATAAGCTTATTTTTAAACAATTTAAAAGCTATTATTCTTAGCATTAACCTATTTTTAAAGAAACCTTTTTTGCTATTGACTGTTTTTTCCCTCTAAGTTTACACTAACATCTACCCAAGATAGACTGTTTTTTAACAGTCAATTTCAGTTCAGCTAACATATATTAATACCTTTGTAACTCTTTGCTATGGCTTTTGTTATCACACCAAAACTATGCAATTGGTACATGGTTGTTTAAGAAGAAACCGTATTTTTCCATGATAAATCACTGTTTGAAATATTTGGTTC

Amino Acid Sequence

MQRDHTMDYKESCPSVSIPSSDEHREKKKRFTVYKVLVSVGRSEWFVFRR
YAEFDKLYNTLKKQFPAMALKIPAKRIFGDNFDPDFIKQRRAGLNEFIQN
LVRYPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSSQKLHSTSQNINLG
PSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLN
RKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFF
HLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLKPENILLDSVGHV
VLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAV
LYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSILEELLEKDR
QNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFD
TAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFLVSKG
EELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLP
VPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA
DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ
SALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP upstream of the SGK3 stop codon. Targets last coding exon (exon 17). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Sequence of cassette depicts the expressed SGK3-GFP fusion.