cDNA Clone SERPINE1

• Synonyms: PAI, PAI-1, PAI1, PLANH1
• Plasmid: pCMV5 SERPINE1 LUC IRES dsRED reporter donor
• Parent Plasmid: pCMV5D FLAG
• Genbank: ENSG00000106366
• Species: Human

Sequence of Insert

gcggccgcCCCTGTCTCTAAAAATGAATGAAAGGAAGGAAGAAAGAGAGAGAAAGAGAGAGAGGAAAGAAGGAAGGAAGTAAAGAAGAAAGAAAGAAAGAAAGAGGAAAGAGGAAGAAAGAAAGAAAAGAAAGAAAAGAAAAGAAAGCAAATTTAAAGCTTATGCAAATCAAAGATGTTGTGATAATTGATAATTGAGTCTGGGCTAAATTCCCCCTGGGCTGCAAAGGCAGAGAGTGGTAATGACTTCTCACCTGCTTTTCTTCTAAGGCTTTTTTACGGGACACAGAGGGAAGGGAGATGGACTGGATTCCAAGATTCCCACAGGGCAAGATGGGCGAAGACTCCCTGCCACTGCCCGGGGATAAGTCAGTCTGAGTGAGACGGAGTGGGATGGGCTTAGAACCTGAACATGTCATGGTCTCTTCCTGCACCTTGCCCTAGTGTTCACTTACCACCTGCTTGCAGGAAACAAGAAGAGCAGGGCCCACAGCTGGCCAGCTCCCCTCCCCTCCCGCCTGTCTTCCAGAACGATTCCTTCACCAGCCCTCTTTCCATTGCTCTAGGATGGAAGATGCCAAAAACATTAAGAAGGGCCCAGCGCCATTCTACCCACTCGAAGACGGGACCGCCGGCGAGCAGCTGCACAAAGCCATGAAGCGCTACGCCCTGGTGCCCGGCACCATCGCCTTTACCGACGCACATATCGAGGTGGACATTACCTACGCCGAGTACTTCGAGATGAGCGTTCGGCTGGCAGAAGCTATGAAGCGCTATGGGCTGAATACAAACCATCGGATCGTGGTGTGCAGCGAGAATAGCTTGCAGTTCTTCATGCCCGTGTTGGGTGCCCTGTTCATCGGTGTGGCTGTGGCCCCAGCTAACGACATCTACAACGAGCGCGAGCTGCTGAACAGCATGGGCATCAGCCAGCCCACCGTCGTATTCGTGAGCAAGAAAGGGCTGCAAAAGATCCTCAACGTGCAAAAGAAGCTACCGATCATACAAAAGATCATCATCATGGATAGCAAGACCGACTACCAGGGCTTCCAAAGCATGTACACCTTCGTGACTTCCCATTTGCCACCCGGCTTCAACGAGTACGACTTCGTGCCCGAGAGCTTCGACCGGGACAAAACCATCGCCCTGATCATGAACAGTAGTGGCAGTACCGGATTGCCCAAGGGCGTAGCCCTACCGCACCGCACCGCTTGTGTCCGATTCAGTCATGCCCGCGACCCCATCTTCGGCAACCAGATCATCCCCGACACCGCTATCCTCAGCGTGGTGCCATTTCACCACGGCTTCGGCATGTTCACCACGCTGGGCTACTTGATCTGCGGCTTTCGGGTCGTGCTCATGTACCGCTTCGAGGAGGAGCTATTCTTGCGCAGCTTGCAAGACTATAAGATTCAATCTGCCCTGCTGGTGCCCACACTATTTAGCTTCTTCGCTAAGAGCACTCTCATCGACAAGTACGACCTAAGCAACTTGCACGAGATCGCCAGCGGCGGGGCGCCGCTCAGCAAGGAGGTAGGTGAGGCCGTGGCCAAACGCTTCCACCTACCAGGCATCCGCCAGGGCTACGGCCTGACAGAAACAACCAGCGCCATTCTGATCACCCCCGAAGGGGACGACAAGCCTGGCGCAGTAGGCAAGGTGGTGCCCTTCTTCGAGGCTAAGGTGGTGGACTTGGACACCGGTAAGACACTGGGTGTGAACCAGCGCGGCGAGCTGTGCGTCCGTGGCCCCATGATCATGAGCGGCTACGTTAACAACCCCGAGGCTACAAACGCTCTCATCGACAAGGACGGCTGGCTGCACAGCGGCGACATCGCCTACTGGGACGAGGACGAGCACTTCTTCATCGTGGACCGGCTGAAGAGCCTGATCAAATACAAGGGCTACCAGGTAGCCCCAGCCGAACTGGAGAGCATCCTGCTGCAACACCCCAACATCTTCGACGCCGGGGTCGCCGGCCTGCCCGACGACGATGCCGGCGAGCTGCCCGCCGCAGTCGTCGTGCTGGAACACGGTAAAACCATGACCGAGAAGGAGATCGTGGACTATGTGGCCAGCCAGGTTACAACCGCCAAGAAGCTGCGCGGTGGTGTTGTGTTCGTGGACGAGGTGCCTAAAGGACTGACCGGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGCAAGATCGCCGTGACTAGTTCTCACGGCTTCCCTCCCGAGGTGGAGGAGCAGGCCGCCGGCACCCTGCCCATGAGCTGCGCCCAGGAGAGCGGCATGGATAGACACCCTGCTGCTTGCGCCAGCGCCAGGATCAACGTCTAAGGTACCGCGGGCCCGGGATCCGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGGACAACACCGAGGACGTCATCAAGGAGTTCATGCAGTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACTACTTCGAGATCGAGGGCGAGGGCGAGGGCAAGCCCTACGAGGGCACCCAGACCGCCAAGCTGCAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACATGAAGCTGTCCTTCCCCGAGGGCTTCACCTGGGAGCGCTCCATGAACTTCGAGGACGGCGGCGTGGTGGAGGTGCAGCAGGACTCCTCCCTGCAGGACGGCACCTTCATCTACAAGGTGAAGTTCAAGGGCGTGAACTTCCCCGCCGACGGCCCCGTAATGCAGAAGAAGACTGCCGGCTGGGAGCCCTCCACCGAGAAGCTGTACCCCCAGGACGGCGTGCTGAAGGGCGAGATCTCCCACGCCCTGAAGCTGAAGGACGGCGGCCACTACACCTGCGACTTCAAGACCGTGTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCAACCACTACGTGGACTCCAAGCTGGACATCACCAACCACAACGAGGACTACACCGTGGTGGAGCAGTACGAGCACGCCGAGGCCCGCCACTCCGGCTCCCAGtgagctagcCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCAGATGTCTCCAGCCCTCACCTGCCTAGTCCTGGGCCTGGCCCTTGTCTTTGGTGAAGGGTCTGCTGTaCAtCAcCCtCCgagtTAtGTaGCCCACCTGGCCTCAGACTTCGGGGTGAGGGTGTTTCAGCAGGTGGCGCAGGCCTCCAAGGACCGCAACGTGGTTTTCTCACCCTATGGGGTGGCCTCGGTGTTGGCCATGCTCCAGCTGACAACAGGAGGAGAAACCCAGCAGCAGATTCAAGCAGCTATGGGATTCAAGATTGATGGTGAGCCACGGGACACCAGGGGAGGTGGGTGGCATGCAGAACAGACCTACCAGAAGCCAAGGAAAGGCTGGCTCTGGCTTAGCCGAGCCAAGCCCCATACAGCTGTGCTGCAGGGGCCACCCCATCTTCTTCCCACTACACTCCAAGTCACTGGACCCTTGAATCTCCAAGGGTGTCTGACCAGTAGATTTACCGCTTATTCACCACCGTGTGATCTTAACCTCGTTAAGTTgcggccgcgtcgac

Amino Acid Sequence

MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVD
ITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGV
AVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKII
IMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSG
STGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMF
TTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTL
IDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAIL
ITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVD
YVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV
TSSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV*........IRES.......MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAKLQVTK
GGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFE
DGGVVEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKL
YPQDGVLKGEISHALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLD
ITNHNEDYTVVEQYEHAEARHSGSQ*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48154
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: LUC-hPEST-IRES-dsRED monomer SERPINE1 reporter (N terminal knockout). Loss of gene expression is lethal thus this donor is to be modified to become a knock-in by inserting an in-frame c-terminal 2A sequence following the dsRED.