cDNA Clone SMAD4

• Plasmid: pCMV5-FLAG-1 SMAD4 K159R
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-SMAD4 K159R
• Genbank: NM_005359
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGACAATATGTCTATTACGAATACACCAACAAGTAATGATGC
CTGTCTGAGCATTGTGCATAGTTTGATGTGCCATAGACAAGGTGGAGAGA
GTGAAACATTTGCAAAAAGAGCAATTGAAAGTTTGGTAAAGAAGCTGAAG
GAGAAAAAAGATGAATTGGATTCTTTAATAACAGCTATAACTACAAATGG
AGCTCATCCTAGTAAATGTGTTACCATACAGAGAACATTGGATGGGAGGC
TTCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGATCTATGCCCGTCTC
TGGAGGTGGCCTGATCTTCACAAAAATGAACTAAAACATGTTAAATATTG
TCAGTATGCGTTTGACTTAAAATGTGATAGTGTCTGTGTGAATCCATATC
ACTACGAACGAGTTGTATCACCTGGAATTGATCTCTCAGGATTAACACTG
CAGAGTAATGCTCCATCAAGTATGATGGTGAgGGATGAATATGTGCATGA
CTTTGAGGGACAGCCATCGTTGTCCACTGAAGGACATTCAATTCAAACCA
TCCAGCATCCACCAAGTAATCGTGCATCGACAGAGACATACAGCACCCCA
GCTCTGTTAGCCCCATCTGAGTCTAATGCTACCAGCACTGCCAACTTTCC
CAACATTCCTGTGGCTTCCACAAGTCAGCCTGCCAGTATACTGGGGGGCA
GCCATAGTGAAGGACTGTTGCAGATAGCATCAGGGCCTCAGCCAGGACAG
CAGCAGAATGGATTTACTGGTCAGCCAGCTACTTACCATCATAACAGCAC
TACCACCTGGACTGGAAGTAGGACTGCACCATACACACCTAATTTGCCTC
ACCACCAAAACGGCCATCTTCAGCACCACCCGCCTATGCCGCCCCATCCC
GGACATTACTGGCCTGTTCACAATGAGCTTGCATTCCAGCCTCCCATTTC
CAATCATCCTGCTCCTGAGTATTGGTGTTCCATTGCTTACTTTGAAATGG
ATGTTCAGGTAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTT
ACTGTTGATGGATACGTGGACCCTTCTGGAGGAGATCGCTTTTGTTTGGG
TCAACTCTCCAATGTCCACAGGACAGAAGCCATTGAGAGAGCAAGGTTGC
ACATAGGCAAAGGTGTGCAGTTGGAATGTAAAGGTGAAGGTGATGTTTGG
GTCAGGTGCCTTAGTGACCACGCGGTCTTTGTACAGAGTTACTACTTAGA
CAGAGAAGCTGGGCGTGCACCTGGAGATGCTGTTCATAAGATCTACCCAA
GTGCATATATAAAGGTCTTTGATTTGCGTCAGTGTCATCGACAGATGCAG
CAGCAGGCGGCTACTGCACAAGCTGCAGCAGCTGCCCAGGCAGCAGCCGT
GGCAGGAAACATCCCTGGCCCAGGATCAGTAGGTGGAATAGCTCCAGCTA
TCAGTCTGTCAGCTGCTGCTGGAATTGGTGTTGATGACCTTCGTCGCTTA
TGCATACTCAGGATGAGTTTTGTGAAAGGCTGGGGACCGGATTACCCAAG
ACAGAGCATCAAAGAAACACCTTGCTGGATTGAAATTCACTTACACCGGG
CCCTCCAGCTCCTAGACGAAGTACTTCATACCATGCCGATTGCAGACCCA
CAACCTTTAGACTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRA
IESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKG
FPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSP
GIDLSGLTLQSNAPSSMMVRDEYVHDFEGQPSLSTEGHSIQTIQHPPSNR
ASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQ
IASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQ
HHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETF
KVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQL
ECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFD
LRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAG
IGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEV
LHTMPIADPQPLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24422
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee