cDNA Clone SLX4

• Plasmid: pcDNA5D FRT/TO mCherry SLX4 D692A H706A Y756A
• Parent Plasmid: pcDNA5D FRT/TO mCherry
• Expressed: mCherry SLX4 D692A H706A Y756A
• Genbank: NM_032444.2 GI:63252862
• Tag: mCherry
• Species: Human

Sequence of Insert

GGATCCATGAAACTGAGTGTGAATGAGGCTCAGCTAGGCTTCTACTTGGG
TTCACTTTCTCATCTGTCTGCCTGTCCTGGGATTGACCCTCGCTCCTCTG
AAGACCAGCCTGAAAGCCTTAAAACTGGTCAGATGATGGATGAGTCTGAT
GAGGACTTTAAAGAACTCTGCGCTAGCTTTTTCCAAAGGGTGAAAAAACA
TGGAATCAAGGAAGTGTCAGGAGAAAGGAAGACACAAAAGGCTGCCTCAA
ACGGCACTCAGATAAGAAGCAAATTGAAAAGGACCAAACAAACTGCTACC
AAGACCAAAACCCTTCAAGGCCCTGCAGAGAAGAAACCTCCGTCTGGCAG
CCAGGCCCCTAGGACTAAAAAGCAAAGGGTAACCAAATGGCAAGCAAGTG
AACCGGCCCACTCTGTGAATGGGGAGGGGGGTGTGCTTGCCTCTGCTCCA
GATCCACCTGTGCTCCGGGAAACAGCACAAAACACCCAGACGGGTAACCA
GCAAGAACCATCGCCAAACCTTTCCAGAGAGAAAACCAGAGAGAATGTGC
CCAACAGCGACTCCCAGCCTCCTCCTTCCTGTTTGACAACAGCAGTGCCA
AGTCCCTCCAAACCCCGCACAGCACAATTGGTCCTACAGCGAATGCAGCA
GTTCAAGAGAGCAGACCCCGAGCGTTTGAGACACGCTTCAGAAGAGTGCT
CCCTCGAGGCTGCGCGGGAAGAAAATGTCCCAAAGGACCCTCAAGAGGAG
ATGATGGCGGGGAATGTGTATGGGCTTGGGCCCCCTGCCCCAGAGAGCGA
CGCTGCGGTGGCCTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCAT
CGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCTGCCAGATT
TGTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGT
GAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTC
AGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGC
AGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCA
GCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGCA
GCAGCCCACCCATGTTCAGCTTCAGTGATCACAGTAGAGGTCTGAAACGG
AGAGGACCCACCAGCAAGAAGGAGCCACGGAAGAGGCGGAAGGTGGACGA
GGCACCGTCCGAGGACCTGCTGGTGGCCATGGCTCTGTCCCGGTCGGAGA
TGGAGCCGGGTGCGGCTGTACCAGCGCTCAGGCTGGAAAGTGCCTTTTCT
GAGAGGATAAGACCAGAAGCAGAGAATAAAAGTCGCAAGAAGAAACCCCC
GGTATCCCCCCCATTGTTGTTAGTCCAGGACTCTGAAACCACAGGCCGAC
AGATAGAGGACCGTGTGGCCCTGCTCCTCTCTGAGGAAGTGGAATTGTCT
AGCACGCCACCACTTCCTGCCAGCAGGATTTTAAAGGAAGGGTGGGAAAG
AGCGGGCCAGTGTCCTCCTCCACCTGAACGCAAGCAGAGCTTTCTGTGGG
AGGGCAGCGCACTGACTGGGGCCTGGGCCATGGAGGACTTCTACACGGCC
AGGCTGGTCCCTCCTCTCGTGCCCCAGCGGCCTGCCCAGGGCCTTATGCA
GGAGCCCGTGCCGCCTCTGGTGCCACCTGAGCACTCAGAGCTGAGCGAGC
GAAGGTCACCCGCTCTCCACGGCACCCCCACTGCAGGCTGTGGCTCCAGG
GGCCCGTCGCCTTCGGCCAGCCAGAGGGAGCACCAGGCCCTGCAGGACCT
CGTGGACCTGGCGAGGGAGGGACTGAGCGCCAGCCCGTGGCCCGGCAGTG
GGGGCCTGGCTGGCTCGGAAGGGACTGCAGGGTTGGACGTGGTGCCCGGC
GGCCTTCCTCTGACTGGGTTTGTGGTGCCATCGCAGGACAAGCACCCGGA
CAGGGGCGGCCGAACCTTGCTCTCCCTCGGGCTGCTGGTTGCTGACTTTG
GCGCCATGGTCAATAACCCACACCTGAGTGCTGTCCAGTTTCAGACGGAC
AGCGGGGAGGTGCTTTACGCCGCCAAGTTCGTGCTTTATGCCCGATGCCC
GCTCCTCATCCAGTATGTGAACAATGAAGGCTTCTCCGCTGTAGAGGACG
GGGTTCTGACCCAGCGTGTCCTGCTGGGTGACGTGAGCACCGAGGCCGCC
CGCACGTTCCTGCACTATCTCGCCACTGCGGACACTGGCCTTCCTCCTGG
CCTTAGCTCTGAGCTGAGCTCCCTGGCCCACAGGTTTGGCGTGAGTGAGC
TCGTTCACCTGTGCGAACAGGTGCCTATTGCCACTGACTCAGAGGGCAAA
CCATGGGAGGAGAAGGAAGCAGAGAATTGCGAAAGCAGGGCCGAGAATTT
CCAGGAACTCTTGAGGTCAATGTGGGCAGATGAAGAGGAGGAAGCGGAGA
CTTTGTTGAAATCCAAGGACCACGAAGAAGATCAAGAAAACGTGAATGAA
GCAGAAATGGAAGAAATTTATGAATTTGCAGCTACTCAGCGAAAGCTTCT
CCAGGAAGAAAGGGCAGCGGGTGCCGGCGAGGACGCTGACTGGCTGGAGG
GTGGCAGTCCGGTTTCTGGGCAACTCCTAGCAGGTGTCCAGGTGCAGAAA
CAGTGGGACAAGGTGGAGGAGATGGAGCCGTTGGAGCCAGGAAGAGATGA
GGCCGCCACCACCTGGGAGAAGATGGGACAGTGCGCTCTCCCGCCACCCC
AGGGCCAGCACTCAGGGGCACGGGGAGCAGAGGCCCCTGAGCAGGAGGCG
CCAGAGGAGGCGCTTGGCCATTCCAGCTGCTCCAGCCCTTCCAGGGACTG
CCAGGCAGAGAGAAAAGAAGGCTCTCTTCCGCACTCAGATGATGCCGGGG
ATTACGAACAGCTCTTCTCATCAACTCAGGGAGAGATCTCAGAGCCGTCC
CAAATAACAAGTGAGCCCGAGGAACAAAGTGGCGCTGTCAGGGAAAGGGG
GCTGGAGGTTTCTCATCGCCTGGCTCCCTGGCAGGCATCTCCACCGCACC
CGTGCCGCTTCCTATTGGGGCCTCCCCAGGGCGGGAGTCCCCGCGGGTCT
CATCACACAAGTGGGTCGTCCCTGTCAACACCCCGGTCCCGTGGCGGAAC
TTCCCAGGTGGGCTCCCCAACCTTGCTGTCTCCAGCTGTGCCATCAAAGC
AGAAAAGGGACAGGAGCATCCTCACGCTGTCTAAAGAGCCAGGGCACCAG
AAAGGCAAAGAGCGTCGGTCCGTGCTGGAGTGCAGAAATAAGGGGGTCCT
GATGTTCCCAGAAAAATCTCTGTCTATTGACCTAACCCAGTCAAATCCTG
ACCATTCGAGCTCCAGATCTCAGAAATCTTCATCCAAACTGAACGAAGAA
GATGAGGTCATCCTCTTACTGGACTCGGATGAGGAGCTGGAGCTAGAACA
AACCAAAATGAAGTCCATTTCTAGTGATCCTCTGGAAGAAAAGAAAGCTC
TAGAAATTAGCCCTAGGTCCTGTGAGCTGTTTTCCATCATTGATGTTGAT
GCAGATCAGGAACCTTCCCAGAGCCCACCAAGAAGCGAAGCTGTGCTGCA
GCAGGAGGATGAGGGGGCGCTGCCGGAGAATCGGGGCTCTTTGGGCAGGA
GAGGGGCTCCCTGGCTGTTCTGTGACCGTGAGAGCAGCCCCAGCGAGGCC
AGCACCACAGACACCTCGTGGCTGGTGCCCGCCACCCCGCTGGCCAGCAG
AAGCCGTGACTGTTCTTCCCAGACCCAAATCAGCAGCCTCAGGAGCGGGC
TGGCCGTGCAGGCGGTGACTCAGCACACGCCCAGGGCCTCAGTAGGAAAC
AGGGAAGGGAACGAAGTCGCACAGAAGTTTTCTGTCATCAGGCCCCAGAC
ACCACCGCCCCAGACACCGTCCTCATGCCTCACTCCCGTCTCTCCAGGAA
CTTCTGACGGCAGAAGGCAAGGCCACAGAAGCCCTTCCCGTCCCCACCCC
GGGGGCCACCCGCACTCCTCTCCGCTGGCTCCACATCCCATCTCAGGGGA
CCGCGCCCACTTCAGCAGGCGGTTCCTGAAACACTCGCCGCCTGGGCCAA
GCTTCCTGAACCAGACCCCAGCGGGTGAAGTGGTGGAAGTCGGAGACAGT
GACGATGAGCAGGAGGTGGCCTCCCATCAGGCCAACAGAAGCCCCCCACT
GGACAGTGACCCCCCAATTCCAATTGACGACTGCTGCTGGCACATGGAGC
CCCTCTCGCCAATTCCCATTGACCACTGGAACCTGGAGCGGACCGGCCCC
CTGAGCACCAGCAGCCCCAGCCGCAGGATGAACGAGGCCGCCGACAGCCG
TGACTGTCGCTCCCCGGGACTCCTGGACACCACCCCCATCCGAGGAAGCT
GCACTACCCAGAGGAAATTGCAAGAGAAGTCCTCGGGCGCGGGCTCCCTG
GGGAACAGCAGGCCGAGCTTTCTGAATTCGGCTCTGTGGGACGTTTGGGA
CGGGGAAGAGCAGAGGCCTCCAGAGACCCCTCCTCCGGCCCAGATGCCAA
GCGCTGGTGGAGCTCAGAAGCCCGAAGGGTTAGAGACACCCAAAGGTGCT
AATCGGAAGAAGAACTTGCCCCCCAAAGTGCCCATAACGCCGATGCCACA
GTATTCCATTATGGAGACGCCGGTGCTGAAGAAGGAACTGGATAGGTTTG
GAGTCCGCCCTCTGCCTAAACGCCAGATGGTTCTGAAGCTGAAGGAGATA
TTCCAGTACACTCACCAGACCCTGGACTCAGACTCCGAGGACGAGAGCCA
GTCCTCACAGCCGCTGTTGCAGGCGCCTCACTGCCAGACCCTCGCCTCCC
AGACCTACAAGCCTTCAAGGGCAGGGGTCCATGCCCAGCAGGAGGCCACC
ACAGGACCTGGGGCCCATAGGCCCAAGGGACCTGCTAAGACCAAGGGCCC
CCGACATCAAAGGAAGCATCATGAAAGCATCACACCCCCAAGCAGGTCGC
CCACCAAGGAGGCACCTCCAGGCCTCAATGATGACGCCCAGATCCCAGCC
TCTCAAGAATCCGTGGCCACCTCTGTGGATGGCAGTGACAGCTCCTTGAG
CTCACAGAGTTCTTCCTCCTGTGAGTTTGGAGCGGCATTTGAGTCTGCAG
GTGAAGAGGAGGGCGAGGGGGAGGTCAGTGCCTCGCAGGCAGCCGTGCAG
GCGGCGGACACAGACGAGGCGCTGAGGTGCTACATCCGCTCCAAGCCGGC
CCTGTACCAGAAGGTGCTGCTGTACCAGCCCTTTGAGCTGCGGGAGCTGC
AGGCAGAGCTGAGGCAGAACGGCCTCCGTGTGTCCTCGCGCAGGCTGTTG
GACTTCCTGGACACCCACTGTATCACCTTCACCACTGCCGCCACCCGCAG
GGAGAAGCTCCAGGGCAGGAGGCGGCAGCCTCGGGGCAAGAAGAAGGTGG
AGCGGAACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMKLSVNEAQ
LGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELCASFF
QRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQGPAEK
KPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRETAQN
TQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRTAQLV
LQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVYGLGP
PAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSAMNVT
RREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQCAVK
MEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKKEPRK
RRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEAENKS
RKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPASRIL
KEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLVPQRP
AQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSASQREH
QALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGFVVPS
QDKHPDRGGRTLLSLGLLVADFGAMVNNPHLSAVQFQTDSGEVLYAAKFV
LYARCPLLIQYVNNEGFSAVEDGVLTQRVLLGDVSTEAARTFLHYLATAD
TGLPPGLSSELSSLAHRFGVSELVHLCEQVPIATDSEGKPWEEKEAENCE
SRAENFQELLRSMWADEEEEAETLLKSKDHEEDQENVNEAEMEEIYEFAA
TQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQWDKVEEMEPL
EPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPEEALGHSSCS
SPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEISEPSQITSEPEEQSG
AVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSPRGSHHTSGSSLSTP
RSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEPGHQKGKERRSVLEC
RNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKLNEEDEVILLLDSDE
ELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDADQEPSQSPPR
SEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEASTTDTSWLVPA
TPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNREGNEVAQKFS
VIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSRPHPGGHPHSSPLAP
HPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEVGDSDDEQEVASHQA
NRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLERTGPLSTSSPSRRMN
EAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGAGSLGNSRPSFLNSA
LWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANRKKNLPPKVP
ITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQTLDSD
SEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHRPKGP
AKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVATSVDG
SDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDEALRCY
IRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTHCITFT
TAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25612
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All SLX4 plasmids MUST be grown at 30 degrees C or less to prevent recombination NM_032444.2 GI:63252862 with changes: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, c2454t, t4500c.