cDNA Clone SYT1

• Synonyms: P65, SVP65, SYT
• Plasmid: pcDNA5DfrtTO FLAG SYT1 K314A K328A
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: Flag-SYT1 K314A K328A
• Genbank: NM_001135805.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGTGAGCGAGAGTCACCATGAGGCCCTGGCAGCCCCGCCTGTCACCACTGTCGCGACTGTTCTGCCAAGCAATGCCACAGAGCCAGCCAGTCCTGGAGAAGGAAAGGAAGATGCATTTTCTAAGCTGAAGGAGAAGTTTATGAATGAGTTGCATAAAATTCCATTGCCACCGTGGGCCTTAATTGCAATAGCCATAGTCGCAGTCCTTTTAGTCCTGACCTGCTGCTTTTGTATCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGAAAGGAAAAAGGAGGGAAGAATGCCATTAACATGAAAGATGTAAAAGACTTAGGGAAGACGATGAAAGATCAGGCCCTCAAGGATGATGATGCTGAAACTGGATTGACAGATGGAGAAGAAAAAGAAGAACCCAAAGAAGAGGAGAAACTGGGAAAACTTCAGTATTCACTGGATTATGATTTCCAAAATAACCAGCTGCTGGTAGGGATCATTCAGGCTGCCGAACTGCCCGCCTTGGACATGGGGGGCACATCTGATCCTTACGTGAAAGTGTTTCTGCTACCTGATAAGAAGAAGAAATTTGAGACAAAAGTCCACCGAAAAACCCTTAATCCTGTCTTCAATGAGCAATTTACTTTCAAGGTACCATACTCGGAATTGGGTGGCAAAACCCTAGTGATGGCTGTATATGATTTTGATCGTTTCTCTAAGCATGACATCATTGGAGAATTTAAAGTCCCTATGAACACAGTGGATTTTGGCCATGTAACTGAGGAATGGCGTGACCTGCAAAGTGCTGAGAAGGAAGAGCAAGAGAAATTGGGTGATATCTGCTTCTCCCTTCGCTACGTACCTACTGCTGGTAAGCTGACTGTTGTCATTCTGGAGGCAAAGAACCTGAAGAAGATGGATGTGGGTGGCTTATCCGATCCTTATGTGGCGATTCATCTGATGCAGAATGGTAAGAGGCTGAAGAAGAAAGCGACAACAATTAAAAAGAACACACTTAACCCCTACTACAATGAGTCATTCAGCTTTGAAGTACCTTTTGAACAAATCCAGAAAGTGCAGGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGATGCCATCGGCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCAGGCGACCTATTGCCCAGTGGCACACCCTGCAGGTAGAGGAGGAAGTTGATGCCATGCTGGCCGTCAAGAAGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDA
FSKLKEKFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKN
KKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKE
EEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPD
KKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHD
IIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGK
LTVVILEAKNLKKMDVGGLSDPYVAIHLMQNGKRLKKKATTIKKNTLNPY
YNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELR
HWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48366
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: K314A + K328A, also contains a single silent nucleotide variation C>T 75bp.