cDNA Clone WNK3

• Synonyms: PRKWNK3
• Plasmid: pMEX WNK3 451-end
• Parent Plasmid: pMEX3C
• Expressed: MBP-3C-WNK3 451-end
• Genbank: NM_020922.4
• Tag: MBP
• Species: Human

Sequence of Insert

GAATTCAGTTTCAACTTAGAAACAGATACACCTGAGGAAGTAGCATATGAAATGGTCAAGTCTGGGTTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAATCCATTAGAGACCGGGTGACGCCAATAAAGAAGACAAGAGAGAAGAAGCCTGCTGGCTGTTTGGAAGAACGCAGGGATTCTCAGTGCAAGTCTATGGGGAATGTATTCCCTCAGCCCCAGAATACAACTTTACCCCTTGCTCCCGCTCAGCAAACTGGGGCTGAATGTGAAGAAACTGAAGTTGATCAACATGTTAGACAACAGCTTCTACAAAGAAAACCACAGCAGCACTGCTCCTCTGTTACAGGTGACAATTTGTCTGAGGCAGGAGCTGCATCAGTTATACATTCAGATACTTCAAGTCAGCCCAGTGTAGCCTATTCCTCAAATCAAACGATGGGCTCTCAAATGGTTTCTAATATCCCGCAGGCTGAAGTAAATGTTCCAGGGCAAATTTATTCATCTCAGCAACTAGTAGGACATTACCAGCAAGTTTCAGGGTTACAGAAGCATTCAAAGCTGACTCAGCCGCAGATTTTGCCTTTGGTTCAAGGTCAGTCCACTGTTTTACCTGTACATGTCCTTGGACCGACAGTTGTTTCACAACCCCAGGTTTCCCCATTAACTGTTCAGAAGGTCCCACAGATAAAGCCTGTATCCCAACCAGTTGGAGCTGAACAACAAGCAGCTCTTCTAAAACCAGATTTAGTTCGAAGCTTGAATCAAGATGTGGCAACTACGAAGGAAAACGTCAGTAGCCCTGATAACCCAAGTGGAAATGGCAAACAGGATCGGATCAAACAGAGAAGAGCTTCCTGTCCCCGACCAGAGAAGGGGACTAAATTTCAGCTTACCGTCCTTCAGGTATCAACCTCTGGAGATAACATGGTAGAGTGTCAGCTGGAGACACACAACAACAAGATGGTCACCTTCAAGTTTGATGTTGATGGTGATGCGCCAGAGGATATTGCAGACTATATGGTTGAAGATAACTTTGTGCTGGAAAGTGAGAAAGAAAAATTTGTAGAAGAATTGAGAGCTATTGTAGGTCAAGCCCAGGAGATCCTTCATGTCCACTTTGCCACAGAAAGAGCCACTGGAGTTGATTCTATTACTGTGGACTCCAACAGTAGCCAGACAGGGTCATCTGAACAAGTACAGATAAATTCTACATCTACTCAAACCAGCAATGAATCTGCTCCTCAGTCATCCCCAGTTGGTCGGTGGCGATTCTGTATCAATCAGACGATAAGAAACCGTGAGACTCAGTCTCCTCCTTCTCTTCAGCATTCCATGTCTGCGGTTCCTGGCCGACATCCACTTCCTAGTCCAAAAAACACAAGTAATAAGGAAATATCACGGGACACATTGATCACTATAGAAAATAATCCATGCCACCGTGCACTTTTCACCTCCAAATCAGAACACAAGGATGTGGTTGATGGTAAGATTTCTGAATGTGCTAGTGTAGAAACCAAGCAGCCAGCTATACTTTATCAAGTGGAAGATAACAGGCAGATAATGGCACCAGTTACTAATAGTTCCAGTTACTCTACTACTTCAGTTCGTGCAGTTCCAGCTGAATGTGAGGGACTCACCAAACAAGCAAGCATATTCATACCTGTGTATCCATGTCACCAAACTGCCAGTCAGGCTGATGCACTTATGTCCCATCCTGGCGAATCAACTCAGACTTCTGGTAACTCTCTTACAACTCTGGCATTTGATCAAAAGCCTCAAACCTTATCAGTACAGCAGCCAGCTATGGATGCAGAGTTTATTTCTCAAGAAGGAGAAACTACAGTGAACACTGAAGCAAGTTCTCCTAAGACAGTCATTCCCACTCAGACCCCTGGCCTTGAACCAACTACCCTTCAACCCACTACTGTCCTGGAATCAGATGGAGAAAGACCTCCAAAACTGGAGTTTGCAGACAACCGAATTAAAACTCTGGATGAAAAATTAAGAAACTTGCTCTATCAGGAGCACAGCATCTCTAGCATCTATCCCGAGAGTCAGAAGGATACCCAAAGCATAGACTCTCCATTTTCTTCCTCTGCTGAAGATACCCTCTCCTGTCCAGTGACAGAAGTCATAGCCATCAGTCACTGTGGAATTAAAGATAGCCCTGTACAATCCCCTAATTTCCAACAGACAGGCTCTAAGCTTCTGTCCAATGTGGCTGCAAGTCAGCCTGCTAATATATCAGTGTTCAAAAGGGACCTGAATGTGATAACTTCTGTACCCAGCGAATTGTGTTTACATGAGATGTCCTCAGATGCTTCACTTCCAGGGGATCCAGAGGCCTATCCTGCTGCTGTGTCAAGCGGTGGAGCCATTCATCTGCAGACAGGAGTGGAAACAGAAGAGATGAGATCAGCAATTGCTCCTGATCCCATTCCTCTGACACGGGAGTCCACAGCTGATACTAGGGCTTTGAATAGATGTAAAGCGATGAGTGGATCATTTCAGCGGGGTCGGTTCCAGGTGATTACAATTCCTCAGCAGCAGTCAGCAAAAATGACATCTTTTGGAATAGAACACATATCAGTGTTCAGTGAGACAAACCATTCTAGTGAAGAAGCCTTTATTAAAACAGCAAAGTCTCAGTTGGTAGAAATAGAACCTGCCACACAAAATCCAAAAACTTCGTTTTCTTATGAGAAGTTACAAGCTCTTCAGGAAACCTGTAAAGAAAATAAAGGAGTTCCCAAACAAGGTGACAACTTCTTATCTTTCAGCGCAGCTTGTGAGACTGATGTATCTTCAGTGACCCCAGAAAAGGAATTTGAAGAAACTTCAGCCACAGGAAGTAGCATGCAGTCTGGATCTGAACTGTTGCTTAAAGAGAGAGAGATATTGACTGCTGGGAAACAGCCTAGCTCTGATAGTGAATTTTCAGCCAGTCTTGCTGGCAGTGGAAAGTCAGTGGCAAAGACTGGTCCAGAGAGTAATCAGTGCTTACCACACCACGAAGAACAAGCTTATGCTCAAACACAGAGTTCACTCTTCTATTCGCCATCTTCCCCAATGAGCAGTGATGATGAATCAGAAATAGAGGATGAGGACTTGAAGGTGGAGCTTCAAAGATTACGAGAAAAACACATTCAGGAGGTGGTAAATCTTCAAACCCAGCAGAATAAGGAGCTGCAGGAGCTCTATGAACGCCTTCGGTCAATTAAAGATAGCAAAACCCAATCTACTGAGATTCCTTTGCCACCTGCATCACCACGTCGACCAAGATCTTTCAAAAGCAAACTTCGAAGCCGCCCCCAGTCCTTGACACATGTGGACAATGGCATAGTTGCTACAGATCCACTGTGTGTGGAGAGTAATGCAGCATCATGCCAACAGTCTCCAGCCAGTAAAAAAGGGATGTTCACAGATGACTTACACAAGCTGGTGGATGACTGGACAAAGGAAGCAGTAGGAAATTCTCTTATTAAGCCAAGTTTAAACCAACTTAAACAAAGTCAACACAAACTAGAGACAGAAAACTGGAATAAAGTATCTGAAAATACTCCGTCTACTATGGGCTACACATCAACATGGATTTCTTCTCTGTCCCAAATCCGTGGAGCTGTCCCAACTTCCTTGCCACAAGGACTCTCACTCCCTTCATTTCCTGGGCCATTATCATCATATGGAATGCCTCACGTTTGTCAGTATAATGCTGTGGCGGGGGCGGGGTATCCAGTACAGTGGGTAGGAATTTCAGGAACAACACAACAATCTGTAGTAATTCCCGCCCAATCTGGGGGACCATTCCAGCCAGGGATGAATATGCAGGCATTTCCAACTTCATCAGTGCAGAATCCTGCCACAATCCCTCCTGGTCCTAAATGAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFP
QVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALM
FNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLP
TFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVI
NAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSPGEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIK
KTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETE
VDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSN
QTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQIL
PLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAA
LLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGT
KFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVED
NFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQT
GSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQH
SMSAVPGRHPLPSPKNTSNKEISRDTLITIENNPCHRALFTSKSEHKDVV
DGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECE
GLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQ
TLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTV
LESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSID
SPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAAS
QPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAI
HLQTGVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQV
ITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQN
PKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEF
EETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKT
GPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQR
LREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRP
RSFKSKLRSRPQSLTHVDNGIVATDPLCVESNAASCQQSPASKKGMFTDD
LHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGY
TSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAG
YPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGP
K*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48423
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 451-end, del. amino acids 1248-1294 and 1614-1623 as compared to reference. Also contains an L470I residue change (equivalent to L920I in full-length).